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Probiotic coadministration with soy beverages has been shown to have favorable results in human health. This study aims to conduct a systematic review of randomized clinical trials to examine the effect of probiotic soy beverages (PSB) on human health aspects such as lipid profile, oxidative stress and kidney function.

The study was conducted based on Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines. Relevant articles were extracted from PubMed, SCOPUS, Web of Science, Science Direct and Google Scholar up to April 2024. This paper searched all databases using the following keywords: (“Soy milk” OR “Soy beverage” OR “Fermented soy milk” OR “Soy Fermented Beverages”) AND (“Probiotic” OR “Probiotics”). The literature search did not consider language, age and publication date restrictions.

A total of 2,183 subjects were included in this review. PSB beneficially decreased low-density lipoprotein cholesterol and total cholesterol levels and increased high-density lipoprotein cholesterol. PSB beneficially affected superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione. PSB decreased serum creatinine and increased estimated glomerular filtration rate. Cystatin-C and progranulin decreased significantly in the PSB group. PSB had no significant effect on anthropometric indices, while decreased systolic and diastolic blood pressure.

This study provides a brief overview of the effect of PSB on lipid profile, oxidative stress, inflammation and kidney function for the first time.

The purpose of this paper is to estimate the influence of long‐term, whole‐body exposure of rats to strong, static electric field with physical parameters generated nearby high voltage direct current (HVDC) transmission lines on the intensity of reactive oxygen species generation analyzed indirectly, basing on the measurement of malone dialdehyde level, as well as on the activity of enzymatic antioxidant defence system.

In rats exposed to static electric field with intensity of 16, 25 and 35 kV/m, respectively, or sham‐exposed eight hours daily for 56 days, in the obtained plasma, erythrocytes lysates and liver homogenates the activity of some antioxidant enzymes as well as the concentration of malone dialdehyde were determined with use of spectrophotometric and kinetic methods.

It was observed that long‐term exposure of rats to static electric field causes only temporary compensatory changes in the concentration of malone dialdehyde and transient changes in the activity of enzymatic antioxidant system, both in blood and in liver tissue in the form of temporary inhibition of activity of most antioxidant enzymes during exposure cycle, with subsequent compensatory increase in this activity after the end of exposure cycle, enabling maintenance of prooxidant‐antioxidant balance in the organism of experimental animals and inhibition of peroxidation process.

Presented data indicate that construction of air HVDC transmission lines, according to actual compulsory regulations, enables serious health hazards related to persistent disturbances of prooxidant‐antioxidant balance to be avoided.

In the experiment it was confirmed, for the first time, that long‐term exposure to strong static electric field causes transient compensatory changes of prooxidant‐antioxidant balance in living organisms.

The purpose of this paper is to investigate the restoration effect of camel's milk against methoxychlor induced liver toxicity.

The present study was carried out to investigate the restoration effect of camel's milk against methoxychlor induced liver toxicity.

Methoxychlor (MXC) caused a significant increase in serum transaminases (aspartate transaminase and alanine transaminase) and alkaline phosphatase, while MXC induced a significant reduction in total protein and albumin levels. MXC significantly inhibited lipid peroxidation and markedly enhanced glutathione in liver homogenate. Pathological damages as degeneration and coagulative necrosis of hepatocytes were established in liver. Newly formed bile ducteules denotes neoplastic changes in the portal tract with abnormal mitotic pattern were associated with the long-term exposure.

The present study concluded that camel milk treatment may play a protective role against methoxychlor-induced liver damage in rats.

The purpose of this study is to determine the effect of olive cake (OC) on lipid peroxidation as well as antioxidant enzymes activities of serum, red blood cells (RBCs) and liver, in streptozotocin (STZ)-induced-diabetic rat fed cholesterol-enriched diet.

Hypercholesterolemic male rats were rendered diabetic (HC-D) by a single intraperitoneal injection dose of STZ (35 mg/kg BW). HC-D rats were divided into two groups fed for 28d a diet supplemented with OC at 7.5 percent (HC-D-OC) or not (HC-D). A control group (C) was submitted to standard diet containing 20 per cent casein for the same experimental period.

RBCs, serum and liver thiobarbituric acid reactive substances (TBARS) contents were significantly increased in HC-D, compared to C group (p = 0.04, p = 0.02 and 0.03). These values were significantly decreased (48 per cent and 64 per cent; p = 0.02 and p = 0.0007) in serum and liver of HC-D-OC vs HC-D group. In RBCs, superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activities were, respectively, 1.5, 2- and 1.7-fold higher (p = 0.03, p = 0.008 and p = 0.03) in HC-D group compared to HC group. In serum and liver, SOD, CAT and GST activities were, respectively, 1.3-, 2.6- and 1.6-fold increased (p = 0.03, p = 0.007 and p = 0.02). In HC-D-OC compared to HC-D group, RBCs glutathione peroxidase (GSH-Px), CAT and GST activities were, respectively, 2.1-, 3.3- and 2.1-fold higher (p = 0.04, p = 0.0009 and p = 0.03). In serum, SOD and CAT activities were, respectively, 1.5- and 1.9-fold increased (p = 0.02, p = 0.02). In liver, SOD, GSH-PX, CAT and GST activities were significantly increased (p = 0.005, p = 0.03, p = 0.02 and p = 0.04).

In diabetic rats-fed cholesterol-enriched diet, OC was able to reduce oxidative stress by decreasing lipid peroxidation and increasing antioxidant enzymes activities in serum, RBCs and liver.

In this study, a novel glutathione (GSH) surface molecular imprinting polymer (SMIP) was successfully prepared by using macroporous adsorption resins (MAR) as substrate, which could separate and purify GSH efficiently.

SMIP was synthesized by chloromethylated modified MAR (LX1180-Cl) as the substrate, N, N’-methylenebisacrylamide (NMBA) as a crosslinker, GSH as a template, acrylamide (AM) and N-vinylpyrrolidone (NVP) as functional monomers. The morphology and structure of the polymer were characterized by scanning electron microscope and Fourier transforms infrared spectroscopy.

The maximum adsorption capacity toward GSH was 39.0 mg/g and the separation decree had relation to L-cysteine (L-cys) was 4.2. The optimal operation conditions were studied in detail and the got as follows: the molar ratios of NMBA, AM, GSH and NVP, were 7.0, 0.8 and 0.5. The optimal time and temperature were 14 h and 40°C, respectively. The Langmuir and pseudo-first-order model were fitting these adsorption characteristics well.

GSH has a diversity of medicinal and bioactive functions, so the purpose of this study representing a method in separate and purify technology of GSH, which provided a way for the development of medicine.

This contribution provided a novel way to separate GSH from L-cys. Under the optimal conditions, the maximum adsorption capacity toward GSH was 39.0 mg/g and the separation decree had relation to L-cys was 4.2.

The objective of this study was to evaluate the influence of Saccharomyces boulardii on the kinetics of fermentation for organic and conventional apple pulp and to verify the effect of the antioxidant quercetin on the response to cellular oxidative stress.

The kinetic parameters, the content of phenolic compounds, the quantity of quercetin and the antioxidant activity were determined during the fermentation process. The effect of quercetin on cellular oxidative stress was also investigated.

The content of phenolic compounds, the antioxidant activity and the quercetin concentration were higher in the organic fermented apple pulp (ORG) than in the conventional fermented apple pulp (CON). However, both apple pulps were considered ideal substrates for the growth of S. boulardii, suggesting that they are potentially probiotic. After fermentation, the quercetin concentration in the ORG treatment and YPDQ treatment (YPD broth with 0.1 mg quercetin rhamnoside/mL) increased viability by 9%, while in the CON treatment generated there was an increase of 6% in viability, compared to the YPD control treatment (YPD broth).

The high concentration of quercetin in the organic apple pulp supports the proposal that quercetin reduces the oxidative stress mediated by reactive oxygen species through its antioxidant action on S. boulardii that have similarities to mammalian eukaryotic cells. These findings suggest that fermented organic apple pulp could be consumed as a potential non-dairy probiotic product.

The purpose of this study was to determine the effects of chickpea (Cicer arietinum) protein hydrolysates prepared at two degrees of hydrolysis (DH) on lipoprotein profile and on oxidant status in cholesterol-fed rats.

Eighteen male Wistar rats (220 ± 10 g) were divided into three groups and fed for 30 days a diet containing 20 per cent casein supplemented with 1 per cent cholesterol and 0.5 per cent cholic acid. During the experimentation, the first and the second groups received daily by gavage 250 mg of chickpea protein hydrolysates/rat at DH = 8 per cent (CPH8) and DH = 17 per cent (CPH17), respectively. The third group, named control group (CG), received water under the same conditions.

Serum total cholesterol concentrations were reduced in CPH8 (p < 0.0073) and CPH17 (p < 0.0004) groups versus CG. This reduction corresponded to a lower very-low-density lipoprotein (VLDL)-cholesterol (p < 0,0019). CPH17 reduced low-density lipoprotein- and high-density lipoprotein (HDL)-cholesterol (p < 0.0001) but increased apolipoprotein A4 (p < 0.002) concentrations and lecithin-cholesterol acyltransferase activity (p < 0.0001). APOA1 remained unchanged in the treated groups. Liver total and esterified cholesterol contents were twofold lower in both treated groups versus CG. CPH8 increased triacylglycerols and phospholipids (p < 0.0001) contents, while CPH17 decreased those of unesterified cholesterol (p < 0.0016). Compared with CG, CPH8 and CPH17 reduced serum (p < 0.0001) and lipoprotein hydroperoxides by stimulating paraoxonase activity (p < 0.0001). However, only CPH17 treatment reduced serum, VLDL- and HDL-malondialdehyde contents and improved glutathione peroxidase activity (p < 0.061).

Thus, chickpea protein hydrolysates and especially hydrolysed at DH = 17 per cent may have a great potential for use as a nutraceutical to reduce hypercholesterolaemia and, by consequence, oxidative stress. Therefore, the degree of enzymatic hydrolysis has a significant influence on the production of potent bioactive peptides.

This paper aims to study the effects of Zygophyllum album (Za) (Z. album) in hypercholesterolemic-diabetic rats.

Male Wistar rats (n = 36) weighing 200 ± 10 g, consumed an experimental diet containing 20 per cent casein were divided into three groups (n = 12). The first group consumed the diet enriched with 1 per cent cholesterol (CH), the second group is rendered diabetic by intraperitoneal injection of streptozotocin (STZ) (35 mg/kg body weight) (D) (DM). The third group is hypercholesterolemic and STZ-induced diabetic (CH-DM); each group was subdivided into two groups (n = 6), non-treated groups (CH, DM and CH-DM) and treated groups supplemented with 1 per cent Z. album lyophilized aqueous extract (CH-Z, DM-Z and CH-DM-Z).

In CH-DM group, Z. album decreases glycemia (−15 per cent) and inversely increases insulinemia (+28 per cent) and Homeostasis Model Assessment of Insulin Resistance (+19 per cent). In liver, total cholesterol (TC) and triacyglycerols (TAG) levels were reduced by −57 per cent and −29 per cent, respectively. In plasma, TC concentration was increased by +20 per cent, whereas those of TAG level were lowered by −56 per cent. Lecithin cholesterol acyl transferase and paraoxonase 1 activities were raised by +45 and +59 per cent, respectively. Inversely, thiobarbituric acid reactive substance levels were lowered significantly in liver, heart, kidney and adipose tissue (p < 0.05). Superoxide dismutase activity was enhanced in liver (+54 per cent), heart (+36 per cent), kidney (+45 per cent) and adipose tissue (59 per cent). Liver glutathione peroxidase (GSH-Px) activity was enhanced by +38 per cent and heart activities of GSH-Px, and glutathione reductase (GSSH-Red) were increased by +25 and +18 per cent. In kidney, GSH-Px activity was reduced by −26 per cent; in reverse, GSSH-Red activity was increased by +14 per cent. In adipose tissue, GSH-Px and GSSH-Red activities were augmented by +35 and +15 per cent.

These results suggest that Z. album aqueous extract has antihyperglycemic and antihyperlipemic actions. Also, Z. album protects against tissue oxidative damage; therefore, it can help to prevent cardiovascular complications of diabetes combined with hypercholesterolemia.

The purpose of this study was to evaluate the antioxidant and hypocholesterolemic activities of sardine and bogue protein hydrolysates in cholesterol-fed rats.

In total, 18 male Wistar rats (220 ± 10 g) fed 20 per cent casein, 1 per cent cholesterol and 0.5 per cent cholic acid were divided into three groups and received a daily gavage of 250 mg of sardine (SPH) or bogue (BPH) protein hydrolysates for 30 days. The third group, named control group (CG), received in the same conditions water. Lipoproteins were fractionated by size-exclusion fast protein liquid chromatography, and serum lipids, apolipoproteins and lipoproteins were assayed.

In SPH and BPH groups, serum total cholesterol concentrations were −66 per cent lower than in CG. This corresponded to the decreased very low-density lipoprotein-C in the former groups. Moreover, BPH treatment reduced low-density lipoprotein-C compared with CG and SPH groups. Compared with CG, serum phospholipids were reduced by SPH and BPH. Furthermore, BPH increased significantly APOA4 and sphingomyelin but lowered phosphatidylcholine. In the latter group, serum lecithin cholesterol acyltransferase activity was +23 per cent higher, but with SPH, this activity was −35 per cent reduced compared with CG. Apolipoprotein A-I contents were similar in the three groups. Compared with CG, hydroperoxide and lipid peroxidation contents in serum and lipoprotein fractions were reduced by SPH and BPH. Compared with CG, serum superoxide dismutase and glutathione peroxidase activities were increased in the treated groups, particularly in the BPH group.

These results suggest that sardine protein hydrolysates and particularly those of bogue could be a very useful natural compound to prevent hypercholesterolemia by both improving the lipid profile and modulating oxidative stress in cholesterol-fed rats.

This study aims to examine the effects of various physical and chemical pre-treatments on antioxidant properties of tree bean (Parkia roxburghii G. Don) in combination with storage conditions.

The whole pods and seeds of tree bean were treated with gamma rays (γ-rays), sodium hypochlorite (NaOCl), chlorine dioxide (ClO₂) and hydrogen peroxide (H₂O₂) and stored at room temperature (RT, 25°C) for 30 days. The physical and chemical pre-treated seeds and pods were compared with the same stored at RT and refrigerated storage at 4°C and −20°C. During storage, physical (moisture content) and antioxidants like total phenolics, ascorbate content, reduced glutathione, total flavonoids, along with free radical scavenging activities (FRSA) were measured.

Chemical pre-treatments with NaOCl, ClO₂ and H₂O₂ significantly accelerated (p = 0.05) the total phenolics (1.9 mg/g FW in seeds and 2.4 mg/g FW in pods), flavonoids content (0.3 mg/g FW each in seeds and pods) and ABTS activities (73.3 per cent in seeds and 92.3 per cent in pods) at 30 days of storage. A significant decrease (p = 0.05) in ascorbate content (6.1 mg/g FW in seeds and 3.5 mg/g FW in pods), reduced glutathione (5.1 mg/g FW in seeds and 3.7 mg/g FW in pods), FRAP (0.3 mg equi Fe/g FW in seeds and 0.4 mg equi Fe/g FW in pods) and reducing power (1.8 mg/g FW in seeds and 3.7 mg/g FW in pods) was observed under all the treatments at 30 days of storage. However, DPPH increased under γ-irradiation and decreased under chemical pre-treatments, storage at RT and refrigerated storage. The overall result showed that pre-treatment of H₂O₂ at 10-20 mM maintains antioxidants and radical scavenging activities in tree bean during storage.

The application of H₂O₂ at 10-20 mM prior to storage of tree bean maintains the physical, antioxidant properties and FRSA in tree bean seeds and pods as compared to natural ambient conditions. Hence, this technique will help in improving the keeping quality of this legume and avoid spoilage after harvest for an extended period.