Study on synthesis and adsorption properties of glutathione surface molecular imprinting polymer

Abstract

Purpose

In this study, a novel glutathione (GSH) surface molecular imprinting polymer (SMIP) was successfully prepared by using macroporous adsorption resins (MAR) as substrate, which could separate and purify GSH efficiently.

Design/methodology/approach

SMIP was synthesized by chloromethylated modified MAR (LX1180-Cl) as the substrate, N, N’-methylenebisacrylamide (NMBA) as a crosslinker, GSH as a template, acrylamide (AM) and N-vinylpyrrolidone (NVP) as functional monomers. The morphology and structure of the polymer were characterized by scanning electron microscope and Fourier transforms infrared spectroscopy.

Findings

The maximum adsorption capacity toward GSH was 39.0 mg/g and the separation decree had relation to L-cysteine (L-cys) was 4.2. The optimal operation conditions were studied in detail and the got as follows: the molar ratios of NMBA, AM, GSH and NVP, were 7.0, 0.8 and 0.5. The optimal time and temperature were 14 h and 40°C, respectively. The Langmuir and pseudo-first-order model were fitting these adsorption characteristics well.

Practical implications

GSH has a diversity of medicinal and bioactive functions, so the purpose of this study representing a method in separate and purify technology of GSH, which provided a way for the development of medicine.

Originality/value

This contribution provided a novel way to separate GSH from L-cys. Under the optimal conditions, the maximum adsorption capacity toward GSH was 39.0 mg/g and the separation decree had relation to L-cys was 4.2.

Citation

Zhang, J., Wang, M., Liu, P., Zhang, X., Huo, T., Chen, Z., Liu, C., Zhang, B., Zhao, S. and Chen, Z. (2021), "Study on synthesis and adsorption properties of glutathione surface molecular imprinting polymer", Pigment & Resin Technology, Vol. 50 No. 6, pp. 585-594. https://doi.org/10.1108/PRT-08-2020-0091