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The purpose of this paper is to optimize production of cellulase enzyme from agricultural waste by using Aspergillus fumigatus JCF. The study also aims at the production of bioethanol using cellulase and yeast.

Cellulase production was carried out using modified Mandel’s medium. The optimization of the cellulase production was carried out using Plackett-Burman and Response surface methodology. Bioethanol production was carried out using simultaneous saccharification and fermentation.

Maximum cellulase production at optimized conditions was found to be 2.08 IU/ml. Cellulase was used for the saccharification of three different feed stocks, i.e. sugar cane leaves, corn cob and water hyacinth. Highest amount of reducing sugar was released was 29.1 gm/l from sugarcane leaves. Sugarcane leaves produced maximum bioethanol concentration of 9.43 g/l out of the three substrates studied for bioethanol production.

The present study reveals that by using the agricultural wastes, cellulase production can be economically increased thereby bioethanol production.

Cellulases and swelling agents are known to be effective in improving the colour strength of cotton. Nowadays, handloom fabrics, such as khadi cotton, are much more preferred due to the development of innovative designs with their use and their comfort in wearing. Also, due to increased environmental awareness, the use of natural dyes are much more preferred in the dyeing of handloom fabrics. However, khadi cotton has some major shortcomings, such as less dyeability. The present study is carried out by keeping in mind that the pretreatment of khadi cotton with cellulases, swelling agents and a combination of cellulases and swelling agents before dyeing improves the colour strength properties. Khadi cotton samples are treated with optimized conditions of the enzymes and swelling agents. The optimum pH, concentration, treatment time and temperature selected for treatment of the samples with acid cellulase enzymes are 5.5, 1.5% (owf), 45 minutes and 50°C, respectively, whereas in the case of neutral cellulase enzymes, 7.5, 2.0% (owf), 70 minutes and 70°C, respectively. The optimum concentration, treatment time and temperature selected for the treatment of the samples with sodium hydroxide, ethylenediamine and zinc chloride are 20% w/v, 60 minutes and 60°C; 80% w/v, 60 minutes and 70°C, and 80% w/v, 60 minutes and 70°C respectively. Butea frondosa dye (5 g) extracted for 75 minutes provides the best results on khadi cotton when dyeing is carried out for 90 minutes. It is observed that out of the various concentrations of mordants used with the Butea frondosa dye, the best shades of colour are obtained by using 0.04 g of alum, 0.01 g of copper sulphate, and 5 g of Babool bark. In terms of optimizing the mordanting, the best results are obtained with Butea frondosa dye when the samples are simultaneously mordanted and dyed with alum, Babool bark and alum. Pre-mordanting is selected for the copper sulphate. It is found that for all the enzyme treated (acid and neutral cellulase) as well as swelling agent treated (sodium hydroxide, ethylenediamine and zinc chloride) samples, the colour strength and colourfastness increase in comparison to the untreated samples.

Enzyme treatment technologies are frequently applied in textile processing for the modification of fabric handle appearance and other surface characteristics in regard to cotton and cotton blended fabrics. The purpose of this paper is to understand the impact of enzyme treatments on fabric preparation, dyeing, and finishing processes of woven fabrics. In particular, certain mechanical and surface properties of 100 percent cotton interlock knitted fabrics after treatment with a cellulase enzyme.

Interlock knitted fabrics were used for this research. These cotton fabrics were treated with experimental Trichoderma reesei cellulases containing different cellulase profiles and treatment was carried out under laboratory conditions. The effects of cellulase treatment on weft knitted fabric regarding mechanical and surface properties were evaluated using the KES‐FB Kawabata evaluation system. The influence of enzyme treatments, friction, and geometrical roughness on the face and reverse side of interlock knitted fabrics were discussed in comparison with untreated interlock knitted fabric.

After each of the enzyme treatments, the interlock knitted fabrics lost part of their weight and, therefore, they became thinner. Furthermore, the extension properties become higher in both directions with regard to the untreated knitted fabric for all used enzymes and carried out treatments.

The paper usefully analyzes changes in the extension and surface properties of enzyme‐treated interlock knitted fabrics by investigating the influence of whole or enriched endoglucanases celullases of Trihoderma reesei under different treatment conditions.

Incorporation of either EDTA or β-cyclodextrin in the bioscouring treatments and its onset on the bioscoured fabrics performance was intensively studied. Biotreatments involved single use of alkaline pectinase enzyme or in combination with cellulase enzyme in a subsequent treatment. EDTA and β-cyclodextrin were entailed independently in the bioscouring by using two strategies: 1) they were applied to the fabrics as a pretreatment and; 2) they were added to the bioscouring treating solution. Fabrics used were enzymatically desized. Desized fabrics under investigation comprised cotton fabric, mercerized cotton fabric, cotton/polyester (50/50) blend fabric and cotton/polyester (35/65) blend fabric. Results showed that pretreatment of fabrics with EDTA followed by subsequent bioscouring by alkaline pectinase enzyme in single use or in combination with cellulase enzyme in a separate step decreases the performance of bioscoured fabrics. On the other hand, incorporation of EDTA in the bioscouring solution containing alkaline pectinase enzyme alone or together with an extra treatment in the bioscouring solution containing cellulase improves the performance of the bioscoured fabrics. It was also found that addition of β-cyclodextrin to the bioscouring solutions containing alkaline pectinase enzyme alone or supported by cellulase enzyme in a separate step acts in favour of the technical properties and performance of the bioscoured fabrics.

The bioavailability of iron from faba bean is low because it is present as an insoluble complex with food components such as phytate, fiber and tannin. The purpose of this paper is to try to identify the nature of the complexes between anti‐nutritional factors and iron in faba bean and legume fractions by using simulations of gastro‐intestinal digestion.

To this aim, the authors evaluated the effect of the action of fiber‐ and/or phytate‐degrading enzymes on solubilization of iron from insoluble residues obtained after gastro‐intestinal digestion of faba bean flour and fractions.

In insoluble residues of raw faba bean flour, simultaneous action of cellulase and phytases made it possible to release about 28 percent units more iron than that released with the treatment without enzymes. About 49.8 percent of iron in raw faba bean flour was solubilized after in vitro digestion and simultaneous action of cellulase and phytase. In the residues of the hull fraction, a significant increase in iron solubility has not been seen (p>0.05) after action of cellulase or phytases. Simultaneous action of cellulase and phytase led to the release of more than 60 and 18 percent units of additional iron for residues of dehulled faba bean and hull fractions, respectively.

In dehulled faba bean, iron was chelated by phytates and fibers. In the hull of faba bean, a high proportion of iron was chelated by iron‐tannins, while the rest of iron was chelated in complexes between phytates and fibers.

Cotton-based fabrics, namely loomstate cotton fabric, grey mercerized cotton fabric, loomstate cotton/polyester (50/50 and 35/65) blended fabric were bio-desized by α amylase enzymes and bio-scoured by alkaline pectinase enzymes. The obtained bio-scoured substrates were subject to bleaching with peracetic acid and proceeded for bio-polishing under a variety of conditions. Results show that the extent of bio-polishing depends on the conditions of the treatment. The loss in fabric weight exhibits values which are comparable at 50° and 60°C and substantially higher than those obtained at 40°C. Temperatures of bio-polishing, specifically 40° and 50°, decrease the whiteness index; higher temperature, i.e. 60 °C, does not cause further decrease in the whiteness index whereas the tensile strength decreases. The temperature has a positive effect on surface roughness particularly when bio-polishing is performed at 50°and 60 °C. It was also found that cellulase is more active in mercerized cotton than in either 100 % cotton or cotton/polyester blend. The scanning electron micrograph of fibers after the enzymatic treatment reveals smoothened faces. The ridges that are present in the untreated fiber samples are not found in the case of cellulase-treated fibers. Bio-polishing of cotton fabrics can offer unmatched results by optimizing the process conditions, which can be otherwise achieved with chemical finishes. Reasonably good results were obtained from all the cotton and cotton blend fabrics and show high flexibility and versatility of the treatment in the manufacturing process.

At present, the enzyme washing process is widely used in hemp garment in the industry. However, it has many disadvantages. First, it is inefficient and labor-intensive, which can only be produced in small quantities and will take much time for workers to check. Second, its cost is high. The strength loss of the garment is up to 20 percent, with a bad damage. Presently, the enzyme washing process of the gray fabric is not industrialized. The purpose of this paper is to obtain a stable and efficient process for the enzyme washing of the gray fabric.

The whiteness, weight loss rate, strength, dyeing property and fabric style of enzyme washed gray fabrics were studied.

The results showed that the enzyme treated fabric has soft handle, smooth surface, good elasticity and high levelness, as well as low strength reduction rate under the optimized condition of bath ratio of 1:12, cellulase dosage of 0.3 percent, pectinase dosage of 1 g/L, treatment temperature of 55°C, treatment time of 80 min and pH of 6.

First, the process is environment friendly, green and sustainable and, second, it will make the industrialization of the enzyme washing process of gray fabrics, with a high commercial value.

Enzymatic treatment of jute fabrics with neutral cellulase enzymes is investigated systematically. The factors affecting enzymatic treatment including, time, liquor ratio, temperature and enzyme concentration are also examined. The hydrolyzed pretreated surfaces of jute fabrics were characterized by scanning electron microscope (SEM), weight loss (WL) and wettability (Wet) tests. WL% and Wet were found to increase as the time, temperature and cellulase concentration increased. SEM images showed that the surface of the treated fabrics was smoother and more polished than that of the untreated fabrics after being subject to optimal treatment conditions.

In contrast, rough surface, fibrillation, surface pilling and multicellular were observed in the images of the untreated fabrics. The dyeing behavior of both untreated and enzymatically treated jute fabrics with reactive dyes was also evaluated under different dyebath conditions. The measured exhaustion and fixation percent showed a significant enhancement of the dyeability of biopolished pretreated fabrics compared to untreated fabrics. The results of fastness properties also indicate good washing, perspiration and rubbing fastness.

This study examines the effects of enzymatic treatment by acid and neutral cellulases after desizing and washing denim fabric. Tensile strength, elongation, stiffness, crease recovery angles and fastness properties of the samples were measured by standard methods. The chemical changes to the fabric samples were observed using the FTIR (Fourier Transformer Infra Red). Also, the surfaces of the treated samples were observed by the SEM (scanning electro microscope). The results show that all these treatments which gave the samples an age-worn appearance decreased the mechanical properties. Acid cellulase is the most aggressive enzyme for specially treated cloths, which proves that washing followed by applying neutral cellulase enzyme is the most suitable treatment for denim fabric.

Examines the tenth published year of the ITCRR. Runs the whole gamut of textile innovation, research and testing, some of which investigates hitherto untouched aspects. Subjects discussed include cotton fabric processing, asbestos substitutes, textile adjuncts to cardiovascular surgery, wet textile processes, hand evaluation, nanotechnology, thermoplastic composites, robotic ironing, protective clothing (agricultural and industrial), ecological aspects of fibre properties – to name but a few! There would appear to be no limit to the future potential for textile applications.