Search results

The purpose of this paper is to investigate the ability of four microorganisms to reduce disease infection of root lesion nematode (Pratylenchus spp.) and Fusarium spp. the causal agent of wilt/root-rot disease complex in chickpea.

A pot experiment was conducted for three consecutive winter seasons. A completely randomized design with five replicates was adopted. Two Bacillus isolates and Trichoderma harzianum and T. viride and their combinations were applied in infected soil. The effect on plant growth parameters, disease incidence and severity, root necrosis, weight of shoot and root, nematode population density and reproductive index were assessed.

The applications significantly (p=0.05) reduced the wilt/root-rot diseases complex. The reduction was attributed to the decline of the population density of nematodes in soil and root, and the suppression of the disease complex compared to controls, represented by reproductive index and the disease intensity index. Combinations were better than individual inoculation. The use of Bacillus isolate B3 and T. harzianum increased the number of flowers by 88.34 per cent. Reduction in the severity of root necrosis was in the range of 2.22-5.55 within a scale of 1-10. These findings indicate the significance of utilizing local bioagents for control of wilt/root-rot disease complex in chickpea plants.

The microorganisms used in this study are indigenous.

The purpose of the paper is to emphasize on contamination sources of freshly cultivated vegetables commonly consumed by the Bangladeshi people. Several local studies have been conducted to detect the microbial contamination within fresh vegetables, plantation lands and the irrigation waters separately; however, the correlation of microbial contamination between the fresh produces and the surrounding environment has not been clarified.

Bottle gourd (Lagenaria siceraria), pumpkin (Cucurbita pepo), radish (Raphanus sativus) and eggplant (Solanum melongena); their plantations soils and the fertilizers applied across the agricultural lands; and, finally, the irrigation waters used were analyzed from nine districts of Bangladesh using conventional microbiological and biochemical methods.

Almost all vegetable samples studied were found to be immensely contaminated with bacteria and fungi. Among the pathogens, Klebsiella spp., Staphylococcus spp. and Pseudomonas spp. were found to be dominant. Besides, massive microbial growth was also observed in the plantation soils and fertilizers, including Klebsiella spp., Pseudomonas spp., Bacillus spp., Listeria spp., Escherichia coli and Vibrio spp. Existence of the fecal coliforms, E. coli, Klebsiella spp., Salmonella spp. and Listeria spp., was noticed in the irrigation waters.

Although the present study revealed the combined results connecting the vegetable contamination aspect with the knowledge on microbiology ultimately in the food chain, implementation of molecular studies detecting the virulence genes both in the fresh produces and the plantation soils, fertilizers and the irrigation waters would further clarify the microbial dissemination mechanism.

Earlier studies demonstrated the ability of water bodies to disseminate numerous microorganisms into the plantation soils, and to some extent unraveled the ability of organic fertilizers to propagate pathogenic bacteria into the vegetation objects. These microorganisms may pose as a threat to vegetables, particularly by limiting crop production as well as the shelf life of the fresh produces.

The scenario of microbial divergence not only in the vegetables but also within the surroundings is gradually being heightened in Bangladesh principally due to the malpractice of sanitation, dumping the agricultural lands with feces, improperly controlled septic systems, waste water runoff across the agricultural lands, etc. Therefore, the preliminary and replicable experimental approach described in the current study would be feasible for all other developing countries to maintain the public health safety.

Growth and proliferation of microorganisms both in the vegetable samples and the environmental samples nearly to a similar extent indeed projected for the first time in Bangladesh, the agricultural perspective of the contamination sources of vegetables. Such knowledge would aid in the existing knowledge on the hygienic processing during crop production and harvesting for the sake of better consumer safety management.

The purpose of this paper is to identify and prioritize the key food safety practices among street food handlers that lead to microbial contamination in selected street foods of Hyderabad, India. These key food safety practices will help develop and design tailor-made training material for street food vendors in future.

It is a cross-sectional study conducted in south Indian city of Hyderabad. Stratified random sampling method was employed. A total of 463 samples of street foods were collected from five zones of Hyderabad. They included 163 salad toppings, 150 fresh fruit juices and 150 panipuri samples. Identification and enumeration of foodborne pathogens and indicator organisms (S. aureus, E. coli, Salmonella spp., Shigella spp., Bacillus cereus, Yersinia spp.) were performed as described by USFDA-BAM. Information on food safety knowledge and handling practices from street vendors were collected using a structured questionnaire. Associations between hygiene practices and bacterial pathogens were done using ANOVA. Risk estimation of food safety practices was assessed by calculating odds ratio.

Microbiological analysis indicated that a large number of carrot (98.1 percent) and onion (75.5 percent) samples were contaminated with E. coli. Peeled and cut fruits left uncovered have 13.4 times risk (OR: 2.40-74.8) of E. coli contamination compared to the covered ones. Panipuri samples picked from the vendors who did not have soap at the vending unit had significantly (p<0.001) higher contamination of fecal coliforms than those who had.

This study is the first of its kind in the study area.

Despite the publication of two comprehensive reports (Richmond I and II) on the microbiological safety of food, together with new legislation, there has been little direct action concerning food safety in the home. Of particular relevance has been new research data which have shown that smaller numbers of bacteria are able to cause food poisoning, which stresses the importance of cross‐contamination. Doubts also have been raised about the safety of cooking raw meat in microwave ovens. Of perhaps greater impact have been the increased demands of food manufacturers and consumers on refrigeration. This has resulted, for example, from the increasing popularity of convenience foods and a reduction in the use of preservatives. Furthermore, many bacteria implicated in food poisoning are able to grow at relatively low temperatures. Recommendations include new standards by manufacturers of refrigerators and microwave ovens, together with a greater awareness and education of food safety issues.

The purpose of this paper is to evaluate the frequency and antimicrobial susceptibility pattern of pathogens present in ready-to-eat salads available at a local market.

A 100 salad samples were collected aseptically. Each sample (25 g) was homogenized in 225 ml of sterile peptone water and was serially diluted up to 1×106. Dilutions were inoculated on nutrient agar by surface spread plate technique. Aerobic colony count (ACC) was estimated by counting the colonies. Bacterial isolates were cultured on blood and MacConkey agar and identified on the basis of their morphology, culture characteristics and confirmed by API 20E and 20NE. Antimicrobial susceptibility was determined as per CLSI 2014.

ACC range was 1.1×103 cfu/g to 5.8×109 cfu/g. Among these the highest ACC was found in channa chat (4.9×104 to 5.8×109 cfu/g). A total of 127 microorganisms were identified; 73 were gram negative rods (GNRs) and 24 were gram positive cocci (GPC). Among GNRs; Klebsiella spp. (n=18) was the predominant whereas among GPC, Staphylococcus aureus (n=6) were the chief pathogen. Klebsiella spp. showed 100 percent resistance to ampicillin, 89-78 percent to amoxicillin/clavulanic acid and 33 percent to imipenem, however, Enterobacter spp. were resistant to ampicillin (100 percent) amoxicillin/clavulanic acid (77 percent) and imipenem (23 percent). Staphylococcus aureus showed resistance to co-amoxiclav (83 percent) and penicillin (75 percent).

This study revealed that effective control measures must been implemented and respected by throughout the entire food chain and better surveillance studies should be performed at national level to reduce the spread of bacteria by fresh salads.

This paper explore the high prevalence of multidrug-resistant pathogens in different salads and most of the salads were found to be unhygienic for consumption.

The purpose of this review is to narrate the microbiological quality of variety of street foods which are largely consumed by the Bangladeshi people of all ages. However, these foods are prone to microbial contamination. Most of the vendors lack the awareness on hygiene during preparing, processing or handling the foods. The insufficiency in regular microbiological analysis further casts the possibility of disease onset. The need of microbial analyses of these foods also remains unclear to the consumers, which, in turn, results in microbial infections and intoxications remaining unnoticed.

The present review focused on the microbiological quality of the street foods projected from the locally conducted researches on street foods, and pondered on the possible management from a microbiological perspective for ensuring consumer safety.

This paper provides comprehensive information on the microbiological quality of street foods, requirement of maintenance of hygiene by the vendors and consumers and the necessity of adopting proper management during food preparation.

Demonstration of microbial prevalence in the street foods may bring imperative information on food safety and security. The conclusive message of this review is about the general consciousness on the microbiological aspects of street food contamination.

The purpose of this study was to investigate the effect of a dietary probiotic on the growth performance and survival rate of Litopenaeus vannamei shrimp. Furthermore, the microbial quality of shrimp was evaluated.

Shrimp were divided into treatment and control groups (each group containing 45 shrimp). They were fed for four weeks with a control diet alone or supplemented with a commercial probiotic (Protexin®). At the end of the trial, they were assessed for survival rate, weight gain, average daily gain and specific growth rate. Samples of tail meat were also provided aseptically from peeled shrimp for bacteriological analysis including the count of Staphylococcus aureus, enterococci, Clostridium perfringens, fecal coliform, Salmonella, Escherichia coli, Listeria monocytogenes and total bacterial count.

The growth performance of the probiotic-treated group significantly (p < 0.05) increased at the end of the experimental period. However, no significant differences were observed for the survival rate between the groups (p > 0.05). The count of C. perfringens and the total bacterial count in shrimp supplemented with the probiotic were significantly lower than those of controls (p < 0.05). The count of coliforms and S. aureus was not significantly different between the groups (p > 0.05).

It could be concluded that the probiotic bacteria have the potential to stimulate the growth performance of L. vannamei. They can also be used for biological control of food-borne pathogens and improve the microbial quality and safety of shrimp at the farm level.

The aim of this paper is to evaluate the storage stability of improved dambu, a steamed granulated dumpling product generally made from millet, was produced from maize (Zea mays), millet (Pennisetum glaucum), sorghum (Sorghum bicolor) and acha (Digitaria exilis). It is a popular mid‐day meal of the Fulanis of Nigeria normally sprinkled into fermented skimmed milk or whole milk and sugar may be added to taste.

Improved dambu was prepared in the laboratory using decorticated clean cereal grains which were pulverished into coarse particles, mixed with spices, preservative (sorbic acid) and water and steamed for 20 min. The improved dambu products were packaged in low‐density polyethylene (LDPE), LDPE with plastic and LDPE with paperboard and stored at room temperature (25 ^○C) for six days. Analysis was carried out on the products following documented and established procedures.

Data obtained indicated that during storage, pH decrease was observed while titratable acidity increased for improved dambu products. The microbial load (cfu/g) increased with storage time for all the products packaged in LDPE, LDPE with plastic and LDPE with paperboard. Dambu has a limited storage life of one day at room temperature (25 ^○C) and four days with 0.2 per cent (w/w) sorbic acid as preservative. Micro‐organisms of significance in the products are Aspergillus sp, Penicillum sp, Candida sp, Staphylococcus aureus and Enterobacter aerogenes. The nature of the microflora suggested that dambu is a good substrate for fungal growth. In the comparative study of dambu products with and without sorbic acid, there was no significant difference in the mean scores for all the assessed parameters.

The consumption of dambu from different cereal grains is encouraged especially where a particular cereal is off season. The shelf‐life of dambu was extended to four days by using 0.2 per cent (w/w) sorbic acid as preservative and packaged in LDPE with plastic and LDPE with paperboard. Further research should be carried out to extend the shelf‐life more.

The findings have suggested that dambu is necessary to cereal industry and baby foods (weaning foods).

The results of this research contributes to the knowledge of cereal meals, especially those that are indigenous to Nigeria and West Africa.

Three events of significance to this country took place in 1899 – the British Food Journal was launched, Australia retained the Ashes, and the Boer War hostilities commenced. If challenged on the order of their importance, cricketers and Empire‐builders may be excused their preference. However, looking at it purely from the standpoint of pro bono publico, the dispassionate observer must surely opt for the birth of a certain publication as being ultimately the most beneficial of the three.

The presence of Bacillus sporothermodurans in retail UHT milk along with milk from different points of a processing line was determined. This paper aims to investigate the effect of chilling, pre‐heating, UHT, reprocessing and H₂O₂ individually and in combination on the survival of B. sporothermodurans in broth.

Standard plate counts were conducted for all milk samples and isolates from UHT milk were characterised using PCR. BS vegetative cells and spores in broth were subjected to various stresses encountered, during UHT processing of milk. Survival counts were conducted after all treatments.

B. sporothermodurans was detected in retail UHT milk packs from only one processor. UHT treatment at 140°C for 4s eliminated B. sporothermodurans in broth. The combination of chilling and UHT was more effective in eliminating B. sporothermodurans spores than UHT treatment alone. H₂O₂ was also effective in eliminating B. sporothermodurans spores after 15 min of exposure. The adopted real time (RT) PCR with SYBR Green method was effective for the confirmation of B. sporothermodurans.

This research is the first to be conducted with regards to the detection of B. sporothermodurans in UHT milk in South Africa and determining the effect of UHT processing stresses on their survival. These results can be used to design processing parameters so as to effectively eliminate B. sporothermodurans spores during UHT processing. This research is the first in which RT PCR with SYBR Green has been used to characterise B. sporothermodurans.