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The purpose of this paper is to investigate the ability of four microorganisms to reduce disease infection of root lesion nematode (Pratylenchus spp.) and Fusarium spp. the causal agent of wilt/root-rot disease complex in chickpea.

A pot experiment was conducted for three consecutive winter seasons. A completely randomized design with five replicates was adopted. Two Bacillus isolates and Trichoderma harzianum and T. viride and their combinations were applied in infected soil. The effect on plant growth parameters, disease incidence and severity, root necrosis, weight of shoot and root, nematode population density and reproductive index were assessed.

The applications significantly (p=0.05) reduced the wilt/root-rot diseases complex. The reduction was attributed to the decline of the population density of nematodes in soil and root, and the suppression of the disease complex compared to controls, represented by reproductive index and the disease intensity index. Combinations were better than individual inoculation. The use of Bacillus isolate B3 and T. harzianum increased the number of flowers by 88.34 per cent. Reduction in the severity of root necrosis was in the range of 2.22-5.55 within a scale of 1-10. These findings indicate the significance of utilizing local bioagents for control of wilt/root-rot disease complex in chickpea plants.

The microorganisms used in this study are indigenous.

This paper aims to discuss about the benefits of Cu(NO₃)₂ applied in coating paint film that consists of plant extract from Cassia Alata. Cu(NO₃)₂ has been added to the coating paint film as a colour stabilizer and as an antifungal property by forming a zone of inhibition towards fungal growth.

The coating paint film resin consists of a mixture of poly (methyl methacrylate) (PMMA) and acrylic polyol. The coating paint film’s performances were observed in terms of visible optical and physical appearance.

When exposed to the fungus Trichoderma Sp., a zone of inhibition of 208 mm² was formed around the coating paint film and therefore it gave the coating paint films an antifungal property. The commission internationale de l’éclairage (CIE) L*a*b* colour coordinate system was used to identify the colour changes in the coating paint film and it was found that the addition of Cu(NO₃)₂ to the coating paint film has a better colour stability, as it has a lower standard deviation (STD) value of 6.46 than the pure dye coating paint film of 8.95. The gloss of coating paint film with the addition of Cu(NO₃)₂ was found to be have a more stable gloss based on its smaller STD value at both angles taken at 20° and 60°.

The Cassia Alata plant has different amount of antifungal properties based on location its growth.

The coating paint film developed in this study is suitable for indoor applications.

The plant extract from the Cassia Alata gives the coating paint film an antifungal property.

This paper is a systemic review on enset plant’s role in Ethiopian people’s life as the source of food. This paper aims to summarize the traditional processing and preparation methods of enset-based foods and their nutritional composition.

Available scientific articles were collected and reviewed for enset plant evaluation, description, enset plant’s role in Ethiopian people’s food security, post harvesting and traditional processing of enset plants, microbiology of the fermented enset foods, different foods reported from enset, nutritional profile of the three food from enset base (kocho, bulla and amicho) and other non-food applications of enset plant.

Enset plant has a predominant role in people living in the southern part of Ethiopia. This plant is drought-tolerant and provides many non-food applications. Harvesting of the enset plant, preparing for fermentation and food preparations follow the traditional route by using the indigenous knowledge and practices. Limited studies have been reported on the microbiology of the enset fermentation, but various types of microbes have been reported. In case of nutritional composition, foods from enset are reported to contain high carbohydrate and minerals content, such as calcium, potassium and zinc, but limited protein content; they are also the best source of the essential amino acids such as lysine and leucine. Limited data are available on vitamins, anti-oxidant and fatty acids profiles of enset-based foods. The existing data indicate variations, and the reasons for variability are discussed in this paper.

Scientific reviews on enset food nutrition profile and related issues are scarce; this paper will compile information about enset plant-based foods for researchers for their future research.

The purpose of this paper is to evaluate the implications of and assess the economic and sustainable impact of environmentally friendly technological packages introduced into agribusiness players in the centre of chilli-producing regions of Indonesia.

This study used a theory of optimisation in production as a fundamental analysis. Producers are assumed to maximise profit by allocating rational amounts of inputs as the components of technological packages. A combination of qualitative and quantitative methods was used. The rate of technology adoption was evaluated, and agribusiness performance was measured using an economic advantage and technical efficiency. Data were compiled using group discussions and individual surveys.

The packages of technologies improved economic and sustainability performance of agribusiness resulting from efficient use of agrochemicals and water resource, and increase the production. Socio-economic and technical factors influenced performance farmers, as agribusiness players responded positively towards the ecological technology packages.

The sustainability of agribusiness was indicated by the reduction in agrochemical use and by the efficient use of water irrigation. There are other measures of sustainability, which are beyond this study. Additional studies are expected to fill the gap.

Considerable potential exists for broad adoption of these technological packages in Indonesia if they are disseminated effectively and in a close partnership with local extension agencies, farmers’ organisations, local non-governmental organisations and private sectors.

This study provides a realistic representation of a current condition because this is an empirical study conducted at the grass-root level. Sustainability of agribusiness practices was achieved with ecological technological packages.

The purpose of this paper is to analyse factors affecting the use of pesticides in intensive vegetable farming in Java, Indonesia. Evaluating such factors is expected to provide appropriate policies to reduce pesticides, and eventually, mitigates the adverse impacts of pesticides on human health and the environment.

Data were compiled from a farm survey of randomly selected 240 vegetable farmers in three regions of Java in 2014. A concept of economic threshold was employed to analyse the pesticide use determinants, which were estimated using econometric models.

Factors leading to the increase in the application of pesticides were the number of observed insect pests, prices of vegetables, use of local varieties, and use of mixed pesticides. Conversely, factors lowering the use of pesticides were the number of observed diseases, the cost of pesticides, and area planted to vegetables. The most important factor in influencing pesticide use was farmers’ perception on the correct prediction of yield losses associated with pests and diseases.

The sample for this research is somewhat low and the analysis was based on one-year data of the quantity of pesticides in a formulation.

The use of pesticides can be reduced by training farmers on crop protection practices, which provide correct information on pests and diseases. Policies related to the price of pesticides would be ineffective, as farmers still highly relied on pesticides. These findings will be useful for reducing the use of pesticides in intensive vegetable farming in Indonesia, and in tropical countries in general.

Pesticides have two opposite properties: to increase income on the one side and to cause devastation of life on the other side. Because pesticides are generally less selectively toxic than would be desired, non-targets including humans and the environment must be protected from contamination by these agrochemicals. This study found the most important determinants for reducing pesticide exposures in Indonesian intensive farming.

This research examines the association of physical development density, prevalence and types of microbes in colonized façade finishes of buildings in Enugu metropolis, Nigeria.

Survey and experimental research designs were adopted. A total of 383 buildings were investigated with samples collected from those with colonized façade finishes. The microbes were identified using the standard procedure for genomic sequencing with descriptive statistics, and the chi-square test used to analyse the data.

The results revealed a 64% prevalence of microbial colonization and a significant association between this and physical development density with 71.0% of the colonized buildings located in high-density neighbourhoods of the metropolis. The sequencing also showed 24 different microbes with Trichophyton tonsurans, Trichophyton mentagrophytes and Trichoderma harzianum species being the most common in the colonized façade finishes.

The research informs building professionals and owners of the specific microbes involved in the colonization of façade finishes of buildings in high-density urban areas. It also provides a clue about the nature of damages and defects associated with microbial colonization of building façades and the type of biocide additives required for the production of microbial-resistant façade finishes in the hot-humid tropical environment of Nigeria and beyond.

The study has shown that there is a significant relationship between the intensity of urban land use and microbial colonization of façade finishes of buildings. It also identified some new or less known microbes responsible for the biodeterioration of façade finishes and the effects this has on the buildings and public health in the hot-humid tropics of Enugu, Southeast Nigeria.

The purpose of this paper is to detect total aflatoxin (AFT) in domestic and imported tea in Amol (north of Iran).

A total of 42 tea samples including 21 domestic and 21 imported tea samples of three popular commercial brands were purchased from retail stores and analyzed for estimation of AFT by ELISA technique. Data were analyzed by ANOVA using SPSS software.

All of the tea samples were contaminated with detectable amount of AFT ranging from 1.5‐16.5 ng/g. The average±SD concentration of AFT in domestic and imported samples was 7.1±2.96 and 6.11±3.94 ng/g, respectively, which is lower than national and EU standards (10 ng/g). The AFT content in 9.5 and 14.3 percent of domestic and imported samples was more than the limited concentration, respectively. There was no significant difference in AFT content of samples of different commercial brands (p>0.05).

No research had been done to detect and compare the AFT content of domestic and imported tea in Iran.

The purpose of this paper is to validate the antimicrobial activity (both antibacterial and antifungal) of in vivo and in vitro ethanolic anthocyanin extracts of Clitoria ternatea L. (vivid blue flower butterfly-pea) and Dioscorea alata L. (purple yam) against selected bacteria (Bacillus subtilis, Staphylococcus aureus and Escherichia coli) and fungi (Fusarium sp., Aspergillus niger and Trichoderma sp.).

The freeze-dried samples (0.2 g) from in vivo vivid blue flowers of C. ternatea L. were extracted using 10 mL ethanol (produced ethanolic red extraction) and 10 mL distilled water (produced aqueous blue extraction) separately. Two-month-old in vitro callus samples (0.2 g) were only extracted using 10 mL ethanol. The anthocyanin extractions were separated with the addition (several times) of ethyl acetate and distilled water (1:2:3) to remove stilbenoids, chlorophyll, less polar flavonoids and other non-polar compounds. Furthermore, the antimicrobial properties were determined using agar diffusion technique. Three bacteria (B. subtilis, S. aureus and E. coli) and fungi (F. sp., A. niger and T. sp.) were streaked on bacteria agar and dextrose agar, respectively, using “hockey stick”. Then, the sterile paper discs (6 mm diameter) were pipetted with 20 µL of 1,010 CFU/mL chloramphenicol (as control for antibacterial) and carbendazim (as control for antifungal) in vivo and in vitro extracts. The plates were incubated at room temperature for 48 h, and the inhibition zones were measured.

Based on the results, both in vivo and in vitro ethanolic extracts from vivid blue flowers of C. ternatea L. showed the best antibacterial activity against the same bacteria (B. subtilis), 11 and 10 mm inhibition zones, respectively. However, different antifungal activity was detected in in vitro ethanolic callus extract (12 mm), which was against T. sp., contrary to in vivo ethanolic extract (10 mm), which was against F. sp.; antibacterial activity of D. alata L. was seen against the same bacteria (E. coli) with the highest inhibition zone for in vivo extract (8.8 mm), followed by in vitro extract (7.8 mm).

Anthocyanins are responsible for the water soluble and vacuolar, pink, red, purple and blue pigments present in coloured plant pigments. These pigments (pink, red, purple and blue) are of important agronomic value in many crops and ornamental plants. However, anthocyanins are not stable and are easy to degrade and fade whenever exposed to light.

Plant extracts containing bioactive agents with antimicrobial properties have been found to be useful in treating bacterial and fungal infections, as well as showed multiple antibiotic resistance.

Both in vivo and in vitro extracts from vivid blue flower petals (C. ternatea L.) and purple yam (D. alata L.) have important applications as natural antimicrobial (antibacterial and antifungal) agents in the coating industry, instead of natural pharmaceutical products.

The purpose of this paper is to review the nutritional and phytochemical value of Moringa oleifera L., along with health benefits. Moringa oleifera, a highly valued plant grown throughout the world and all parts of tree used in different food formulations, possess industrial and therapeutic uses. This plant is gaining popularity because of its nutrient-rich root, leaves, flowers and fruits, having immense traditional medicinal uses and proved pharmacological properties.

Major well-known bibliometric information sources such as Web of Science, Scopus, Mendeley and Google Scholar were searched with keywords such as nutrition value of Moringa oleifera, bioactive compounds, health benefits, processing and safety were chosen to obtain a database of 1,386 papers. A final database of 70 scientific sources was made after sorting and classifying them according to different criteria based on topic relevance, country of origin and year of publication.

The literature reflects that Moringa contains all necessary macro, micro-nutrients and bioactive compounds (terpenoids, polyphenols, flavonoids, glucosinolates, alkaloids, glycosides and carotenoids). Scientific studies illustrate that M. oleifera and its bioactive constituents could play a vital role in the prevention of several chronic and degenerative diseases associated with oxidation stress. The recent upsurge in consumer interest for health foods has opened up new vistas for plant products containing bioactive compounds in different food formulations.

This paper highlights phytochemicals, pharmacological properties, bio-accessibility, food and industrial applications of Moringa. Moringa pods are traditionally preferred for enlarged liver and spleen, intestinal worms, weakness, neurological disorders and skin disease. A seed is natural and an inexpensive coagulant used to remove organic particles from water.

The prayer against the Poultry (Hygiene) Regulations which we briefly mentioned in the editorial of our last issue, was lodged as a result of activity by the Environmental Health Officers' Association. Incidentally it is the first occasion as far as we can recall that a prayer has been lodged against any of the rash of food regulations of recent years, and reflects the strong feelings of the public health inspectorate.