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Acrylamide (AA) is predominantly used as a synthetic substance within various industries. However, AA is also recognized as a carcinogen. Zinc oxide nanoparticles (ZnO-NPs) are becoming increasingly attractive as medical agents. However, to the knowledge, the effects of ZnO-NPs on preventing cytotoxicity with AA have not been reported. Therefore, this study aims to determine the protective effects of ZnO-NPs against the cytotoxicity caused by AA.

MTT assay was used to determine the cytotoxicity. Reactive oxygen species (ROS) formation, carbonyl protein, malondialdehyde (MDA) and glutathione (GSH) were measured and analyzed statistically.

The findings observed that the presence of 200 µM AA led to a substantial reduction in cell viability (p < 0.001). However, ZnO-NPs restored cell viability at 50 and 100 µM concentrations (p = 0.0121 and p = 0.0011, respectively). The levels of ROS were significantly reduced (p = 0.001 and p = < 0.001) to 518 ± 47.57 and 364 ± 47.79, respectively, compared to the AA group. The levels of GSH were significantly increased (p = 0.004 and p = 0.002) to 16.9 ± 1.3 and 17.6 ± 0.5, respectively, compared to the AA group. The levels of MDA were significantly decreased (p = 0.005, p < 0.001 and p < 0.001) when compared to the AA group, as were the levels of carbonyl protein (p = 0.009 and p < 0.002) in comparison to the AA group.

In summary, the outcomes of this research indicate that ZnO-NPs played a role in inhibiting AA-induced oxidative stress and cytotoxicity.

This study aims to examine the relationship between the chemical composition of juices obtained from fruits of autochthonous wild pomegranate (Punica granatum L.) grown in Montenegro and their cytotoxic effects on cancer cells.

To explore the potential value of wild pomegranate fruits, in vitro biological assays were carried out with juices whose composition was analyzed in detail for sugars, organic acids, vitamin C and phenolic compounds. The effect of juices on survival was determined in human lung A549, cervical HeLa and breast MCF-7 carcinoma cells by MTT assay. As a control, the cytotoxicity against normal fetal lung fibroblasts (MRC-5) was monitored.

Among cancer cell lines, considering the IC50 related to total phenolics, the lowest value – 13 µg/mL was found for the A549. The strongest effect on lung cells was assumed due to the favorable contribution of ellagitannins to total phenolics in juice as well as the given combination of anthocyanins and their synergistic action. For HeLa cells, the lowest IC50 value was obtained at 88 µg/mL, and the cytotoxicity could be matched with the effects of anthocyanins and catechin. For MCF-7 cells, the lowest IC50 was 504 µg/mL, and the elevated levels of vitamin C and ellagic acid derivatives should have a noticeable effect on these cells.

This study provides an important contribution to the knowledge on the effect of phytochemicals from wild pomegranate juice on lung, cervical and breast cancer cells, in vitro. The present observations suggest that the juice of wild pomegranate has the potential in the fight against cancer.

This study aimed to evaluate the compositional changes and bioaccessibility of phenolics and antioxidants in propolis during in vitro digestion as well as the cytotoxic effects of digested propolis on various cancer cell lines.

Six propolis samples were obtained and subjected to in vitro oral, gastric and intestinal digestion. Both digested and undigested samples were analyzed for their total phenolic, flavonoid and antioxidant activities. Additionally, changes in phenolic composition in the in vitro digestion system were revealed by the HPLC-DAD system. The cytotoxic effects of the digested samples were assessed on lung (A549, H1299), skin (A431), liver (Hep-G2) and colon (Caco-2) cancer cells as well as on fibroblast (Bj) cells.

The mean bioaccessibility values of phenolic and flavonoid compounds were found to be less than 35 and 24%, respectively, while the TEAC and CUPRAC antioxidant results ranged between 225.08–649.04 and 398.68–1552.28 µmol TE/g, respectively. The release of p-coumaric, ferulic, 3,4-dimethoxycinnamic acids, naringenin, pinocembrin and chrysin increased progressively from the oral to the intestinal stage. The cytotoxic effects of samples on cell lines were ranked, based on IC50 results, as A431 > Hep-G2 > Caco-2 > A549 > H1299 > Bj.

Propolis has been recognized for centuries as a natural remedy, and numerous studies have explored its bioactive components. However, no studies have previously examined the changes in the phenolic compositions of propolis samples during digestion or their cytotoxic effects on cancer cells. Therefore, this study provides novel insights and an approach to the existing literature on this topic.

The purpose of this paper is to investigate the effect of plasma electrolytic oxidation (PEO) coatings and sealed PEO coatings on the corrosion resistance and cytocompatibility of a novel Mg-1Zn-0.45Ca alloy in simulated body fluid (SBF).

The microstructure, corrosion resistance and cytocompatibility of PEO coatings and phosphate conversion-treated PEO coatings were investigated and was compared with the bare Mg alloy.

The hot-extruded Mg-Zn-Ca alloy exhibit inhomogeneous microstructure and suffered from localized corrosion in the SBF. The PEO coating after phosphate conversion treatment offers enhanced protectiveness to the Mg alloy within an immersion period of up to 60 days, which is significantly improved compared with the performance of the PEO-coated Mg alloy, but the cytocompatibility was slightly decreased.

This work offers new perspective in balancing the protectiveness and cytocompatibility of bio-materials.

This study aims to design and implement a multimaterial system for printing multifunctional specimens suitable for various sectors, with a particular focus on biomedical applications such as addressing mandibular bone loss.

To enhance both the mechanical and biological properties of scaffolds, an automatic multimaterial setup using vat photopolymerization was developed. This setup features a linear system with two resin vats and one ultrasonic cleaning tank, facilitating the integration of diverse materials and structures to optimize scaffold composition. Such versatility allows for the simultaneous achievement of various characteristics in scaffold design.

The printed multimaterial scaffolds, featuring 20 Wt.% hydroxylapatite (HA) on the interior and poly-L-lactic acid (PLLA) with 1 Wt.% graphene oxide (GO) on the exterior, exhibited favorable mechanical and biological properties at the optimum postcuring and heat-treatment time. Using an edited triply periodic minimal surface (TPMS) lattice structure further enhanced these properties. Various multimaterial specimens were successfully printed and evaluated, showcasing the capability of the setup to ensure functionality, cleanliness and adequate interface bonding. Additionally, a novel Gyroid TPMS scaffold with a nominal porosity of 50% was developed and experimentally validated.

This study demonstrates the successful fabrication of multimaterial components with minimal contaminations and suitable mechanical and biological properties. By combining PLLA-HA and PLLA-GO, this innovative technique holds significant promise for enhancing the effectiveness of regenerative procedures, particularly in the realm of dentistry.

This study aims to produce composite pigments, including SHS/ZnAl-LDHs, IDS/ZnAl-LDHs and SNND/ZnAl-LDHs, with improved coloration, enhanced photostability and thermostability and biocompatibility.

The chemical structures of the composite pigments were characterized by X-ray diffraction spectroscopy and Fourier transform infrared spectroscopy. Photostability and thermal stability were assessed using ultraviolet-visible spectroscopy and colorimetry. The coverage of the dyes was determined through black-and-white tile testing, and specific RGB values were used to indicate color expressiveness. Finally, a four-color eyeshadow was formulated, and safety tests were conducted via human patch test and cellular assays to confirm the safety and reliability of the samples.

The experimental results demonstrate an enhancement in the photo and thermal stability of the SHS/ZnAl-LDHs, IDS/ZnAl-LDHs and SNND/ZnAl-LDHs composites, along with their superior performance in terms of covering power and color saturation. These composite pigments also exhibit high safety, making them well-suited for cosmetic applications.

The composite pigments based on hydrotalcite can be used in the cosmetic industry without causing any harm to the environment and human health.

The addition of hydrotalcite enables better application of pigments in cosmetics.

The purpose of this paper is to systematically review the functionalities, safety regulations and product applications of herb Stevia rebaudiana extract. This plant material is embedded with multiple functionalities such as antioxidant, antidiabetics, anti-inflammation and antimicrobial. The regulations released from global authorities are covered to ensure the safety premise of stevia. Besides, the product applications of the extract of aerial parts of the herb S. rebaudiana helps us to recognize its value from commercial side.

Relevant literatures are selected and obtained from main scientific databases such as Google Scholar, Web of Science, PubMed and trade magazines published between 2000 and 2023. The keywords and their possible combinations such as sweetening, antioxidant, antidiabetics, anti-inflammation, safety and product development were used to ensure the preciseness and completeness of literature searching. Major data such as sweetness, total phenolic content and dose together with latter critical conclusions from searched publications were appropriately used and discussed. In this review, approximately 150 scientific literatures were meticulously ordered and analyzed. In applications, it is the first time that sentiment analysis was used to obtain a market assessment of the stevia-containing products.

This review paper helps rearrange the scientific affairs of those stevia extract’s functions like sweetening, antioxidant, antidiabetics and inflammation. Sweetness indexes of steviol glycosides were summarized together for comparison while various in vitro and in vivo approaches were reviewed to quantify those functions’ capacities and to depict the related mechanism. The regulation of steviol glycoside compounds such as rebaudioside A was established by global authorities such as US Food and Drug Administration and Joint FAO/World Health Organization Expert Committee to ensure the safety endorsement before commercialization. Then, this study discussed about the market performance of stevia ingredients or products with the self-developed data analytics. This study also investigated the product development progress of stevia-containing food products in the categories of beverage, bakery, dairy and confectionery. Those stevia-containing food consumer goods can be acceptable by certain consumers.

This review paper precisely presents the evidential information about the stevia’s multiple functionalities with mechanisms and global regulation milestones. To the best of the authors’ knowledge, it is then the first time to probe the stevia-containing products’ market performance through data analytics.

This study aims to cover the overall gamut of rapid prototyping processes and biomaterials used for the fabrication of occlusal splints in a comprehensive manner and elucidate the characteristics of the materials, which are essential in determining their clinical efficacy when exposed to oral surroundings.

A collective analysis of published articles covering the use of rapid prototyping technologies in the fabrication of occlusal splints, including manufacturing workflow description and essential properties (mechanical- and thermal-based) evaluation of biocompatible splinting materials, was performed.

Without advances in rapid prototyping processes and materials engineering, occlusal splints would tend to underperform clinically due to biomechanical limitations.

Three-dimensional printing can improve the process capabilities for commercial customization of biomechanically efficient occlusal splints.

Rapid technological advancement in dentistry with the extensive utilization of rapid prototyping processes, intra-oral scanners and novel biomaterial seems to be the potential breakthrough in the fabrication of customized occlusal splints which have endorsed occlusal splint therapy (OST) as a cornerstone of orthodontic treatment.

This study aims to measure the global research landscape of the National Institute of Pharmaceutical Education and Research (NIPER) of India on a set of quantitative and qualitative metrics in terms of research output toward exploring research trends and give an overview of collaborative practices by researchers of NIPERs.

The present study has selected the Scopus database as a tool to retrieve potential publications of studied NIPERs during the last 12 years (2010–2021). NIPER-Mohali, NIPER-Hyderabad, NIPER-Ahmedabad, NIPER-Guwahati and NIPER-Kolkata have been selected for the study. The study has adopted a comprehensive search strategy to extract 3,926 publications data. VOS viewer 1.6.17, BibExcel and Microsoft Excel were used for data analysis and visualization.

The global scientific research output of NIPERs accrued 3,926 publications with an average of 327 publications per year. The retrieved publications fetched a total of 67,772 citations with an average citation impact of 17.26. There observed a steady growth of publications from 168 to 509 registered with an average growth rate of 18.44%. The mean relative growth rate and doubling time of research output are 0.26 and 2.94. The authorship patterns explore collaborative trends as most of the publications were published by multiple authors (99.39%). NIPERs have expanded their outreach to collaborate with the USA, Malaysia, Saudi Arabia, Australia and the UK to collaborate on research and regulatory reforms exhibits in the USA as a major contributor.

The present study is the first effort to evaluate the global research productivity of NIPERs and assess the current research trends on a set of quantitative and qualitative metrics to provide some insights into the complex dynamics of research productivity. The study’s outcome may help to identify the current research progress of NIPERs at the global level.

The purpose of this paper is to review phytochemical potential of acacia and its associated health advantages. Acacia a moderate-sized, deciduous tree and recognised as health-promoting species because of availability of essential bioactive components. The bioactive compounds such as tannins, flavonoids, alkaloids, fatty acids and polysaccharides (gums) present in the plant parts of acacia, namely, bark, leaves, flowers, fruits, twigs and seeds, have medicinal value and thus are used to overlay the formulations of plant-based drugs and value-added foods.

Major well-known bibliometric information sources such as Web of Science, Scopus, Mendeley and Google Scholar were searched with keywords such as “nutrition value of acacia”, “bioactive compounds”, “health benefits”, “processing and safety” were chosen to obtain a database of 1,428 papers. The search considered papers in the English language from the past 18 years of publication in journals (2004–2022). The article selection process consisted of the screening of titles and abstracts, based on inclusion and exclusion criteria. Articles that did not have acacia components as a study objective were taken into consideration for exclusion. A final database of 87 scientific sources was made after sorting and classifying them according to different criteria based on topic relevance, country of origin and year of publication. Articles with other random descriptors were also searched to complement the discussion of the results obtained.

The literature reflected that acacia contains all necessary phytochemicals like polyphenols, flavonoids, terpenoids, glucosinolates, alkaloids and carotenoids along with essential macro, micro-nutrients. Furthermore, processing methods such as soaking, cooking, roasting and dehusking significantly reduced the anti-nutritional factors present in acacia seeds of different species. This review also focused on the processing methods that are used to eliminate or lower down the anti-nutritional factors from the seeds. Previous findings related to acacia plant parts with respect to food development are explored and mentioned.

This review emphasised mainly on recent studies that had been reported on ethnomedical acacia plants therapeutically, commercially and exponentially for further studies to increase the utilisation in food processing.