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Monocytes are a leukocytes’ subset that plays an important role in immunity. Protein kinase B (AKT) is involved in monocytes' survival, proliferation and differentiation. Using phorbol 12-myristate 13-acetate (PMA) as an inducer for cell line U937 differentiation into macrophage-like cells may be used as a model for cancer cell therapy or other biomedical research studies. The authors investigated the Akt1 signaling pathway's involvement with PMA as a differentiating agent and survival in the U937 cell line.

PMA was utilized to stimulate the differentiation of the U937 cell line into macrophage-like cells at a concentration of 10 nM. Akt1-phosphorylated Serine 473, Bad-phosphorylated Serine 136 and Caspase9-phosphorylated Serine 196 were tested by flow cytometry for the involvement of the Akt1 signaling pathway during differentiation in addition to the expression of CD14, CD206 and CD83. DNA cell cycle variation analysis was done using PI staining and cell viability and apoptosis detection using Annexin V and PI flow cytometry.

There was a decrease in phosphorylated Akt1 and Bad activation and an increase in Caspase9 activation, with an increase in surface markers CD14, CD206 and CD83 acquired by PMA-differentiated cells. DNA cell cycle analysis revealed cell accumulation in the G2/M phase and fewer cells in the S phase of PMA-induced U937. Apoptosis induction for Ly294002 or Wortmannin-inhibited cells and part of PMA-induced cells were detected.

These results may be used to create a model for biomedical research studies and advance the understanding of the mechanism involving differentiation of the U937 cell line.

The 3 D bioprinting technology is used to prepare the tissue engineering scaffold with precise structures for the cell proliferation and differentiation.

According to the characteristics of the ideal tissue engineering scaffold, the microstructural design of the tissue engineering scaffold is carried out. The bioprinter is used to fabricate the tissue engineering scaffold with different structures and spacing sizes. Finally, the scaffold with good connectivity is achieved and used to cell PC12 culture.

The results show that the pore structure with the line spacing of 1 mm was the best for cell culture, and the survival rate of the inoculated cells PC12 is as high as 90%. The influence of the pore shape on the cell survival is not evidence.

This study shows that tissue engineering scaffolds prepared by 3 D bioprinting have graded structure for three-dimensional cell culture, which lays the foundation for the later detection of drug resistance.

Studies the effects of exposure to light of the laser diode Melles Griot (λ = 670nm), He‐Ne laser (λ = 632.8nm) and argon laser (λ = 514nm) on selected soil micro‐organisms, fungi that destroy old manuscripts, pictures, stone, etc. and on humification and mineralization of soil samples. Also studies exposure effects on seed growth and biomass production of a few species of cultivated plants and on Chlorella cells and animal spermatozoa. Finds significant changes in comparison to control material (including results of the preliminary measurement of bio‐photon emission). Suggests a fruitful direction for studies on the synergistic effects of Se, laser and white light, as well as on the optimal level of exposure of living material to laser light. Concludes that the data obtained seem to be useful both for land reclamation and for the protection of the indoor environment against toxicogenic moulds and bacteria.

The purpose of this paper is to propose a planning strategy for the radio frequency ablation (RFA) treatment of hepatic tumors. The goal is to give to the surgeon the opportunity of controlling the shape and the size of the treated volume and preserving the healthy tissues.

A FEM model of the human torso is built from radiographic and MRI scans of the patients, and then the RFA treatment “dynamically optimized” by controlling currents in multiple external electrodes, in such a way to drive currents in the desired regions, burning the tumor while trying to preserve healthy regions. A suitable cellular death model is considered in order to achieve an effective description of the biological modifications in the tumor volume.

A numerical method to plan the RFA treatment of hepatic tumors has been defined, aiming to preserve as much as possible healthy tissues.

The method depends on the knowledge of inner structure and properties of the patient's torso; while the structure of tissues can be determined by TAC or MRI scans, the physiological properties are much more uncertain.

The proposed approach allows optimized RFA treatments to be designed, allowing reduction of damage to healthy tissues deriving from application of the treatment.

The problem of optimal design of RFA treatments has been previously tackled in literature, but in this paper, dynamical optimization techniques and a cell death rate model have been included.

Coronavirus disease-2019 (COVID-19) is becoming a crucial health problem worldwide. Continued and high-speed mutations of this virus result in the appearance of new manifestations, making the control of this disease difficult. It has been shown that well-nourished patients have strong immune systems who mostly have short-term hospitalization compared to others. The purpose of this study is to review the major nutrients involved in the immune system reinforcement and to explain nutritional aspects during the recovery of COVID-19.

In this review paper, the mechanistic role of nutrients in boosting the immune system and the nutritional aspects during the recovery of COVID-19 patients were discussed. Papers indexed in scientific databases were searched using antioxidants, COVID-19, inflammation, immune system, macronutrient, micronutrient and probiotic as keywords from 2000 to 2022.

Because of the adverse effects of drugs like thrombosis, pulmonary embolism and hypercholesterolemia, a balanced diet with enough concentrations of energy and macronutrients could increase the patient's durability. The inflammatory cytokines in a vicious cycle delay patients’ rehabilitation. The main mechanistic roles of micronutrients are attributed to the downregulation of virus replication and are involved in energy homeostasis. Dysbiosis is defined as another disturbance among COVID-19 patients, and supplementation with beneficial strains of probiotics helps to exert anti-inflammatory effects in this regard. Being on a well-planned diet with anti-inflammatory properties could reverse cytokine storms as the major feature of COVID-19. Future studies are needed to determine the safe and effective dose of dietary factors to control the COVID-19 patients.

Being on a well-planned diet with anti-inflammatory properties could reverse cytokine storms as the major feature of COVID-19. Future studies are needed to determine the safe and effective dose of dietary factors to control the COVID-19 patients.

The purpose of this paper is to present a topology-based tissue scaffold design methodology to accurately represent the heterogeneous internal architecture of tissues/organs.

An image analysis technique is used that digitizes the topology information contained in medical images of tissues/organs. A weighted topology reconstruction algorithm is implemented to represent the heterogeneity with parametric functions. The parametric functions are then used to map the spatial material distribution following voxelization. The generated chronological information yields hierarchical tool-path points which are directly transferred to the three-dimensional (3D) bio-printer through a proposed generic platform called Application Program Interface (API). This seamless data corridor between design (virtual) and fabrication (physical) ensures the manufacturability of personalized heterogeneous porous scaffold structure without any CAD/STL file.

The proposed methodology is implemented to verify the effectiveness of the approach and the designed example structures are bio-fabricated with a deposition-based bio-additive manufacturing system. The designed and fabricated heterogeneous structures are evaluated which shows conforming porosity distribution compared to uniform method.

In bio-fabrication process, the generated bio-models with boundary representation (B-rep) or surface tessellation (mesh) do not capture the internal architectural information. This paper provides a design methodology for scaffold structure mimicking the native tissue/organ architecture and direct fabricating the structure without reconstructing the CAD model. Therefore, designing and direct bio-printing the heterogeneous topology of tissue scaffolds from medical images minimize the disparity between the internal architecture of target tissue and its scaffold.

The purpose of this paper is to study the effect of heat adaptation, pH adjustment, and the combination pretreatments to the resistance of Lactobacillus paracasei SNP2 in heat shock and spray drying.

Sub-lethal and lethal temperature range of L. paracasei SNP2 was determined by enumeration of cell survival after incubation at 37-55°C for 30 min. A certain temperature from sub-lethal range was selected for heat adaptation. Heat adaptation (H), pH adjustment (pH), and combination of pretreatments (pH-H) were applied prior to heat shock and spray drying.

The selected condition for heat adaptation and heat shock is 44°C, 30 min and 55°C, 15 min, respectively, based on the sub-lethal and lethal temperature range. By heat shock, cells in the whey-sucrose medium showed cell death of 2.05 log cycles, lower than cell death in the MRS medium of 4.84 log cycles. The pretreatments showed slight increase of heat resistance in cell grown in whey sucrose. The effect of H, pH, and pH-H pretreatments highly increase heat resistance of cell grown in MRS indicated by cell death of 4.27, 3.79, and 2.43 log cycles, respectively, which is much lower than control. The pretreatments showed no significant effect to L. paracasei SNP2 survival to spray drying.

This is the first study of L. paracasei SNP2 resistance to heat shock and spray drying. This paper also enriches information about application of whey sucrose as a growth medium and a heating medium.

The purpose of this paper is to evaluate the protective effects of Persian gum and gum Arabic on the survival of Lactobacillus plantarum subsp. plantarum PTCC 1896, Escherichia coli, Xanthomonas axonopodis, and Saccharomyces cerevisiae during freeze drying.

Cultures were harvested from the early stationary phase and enumerated after dilution according to the Milse Misra method. Bacterial suspensions were mixed with protective agents and frozen at –80°C before drying in a freeze dryer. Survival rates were determined both immediately during freeze drying and after 14 days of cold storage (at 4°C).

Compared to gum Arabic 5 and 10 percent (W/V) or skim milk 10 percent (W/V), Persian gum 1 percent (W/V) showed no significantly different effects on the survival of Lactobacillus plantarum subsp. plantarum PTCC 1896 (p<0.05). Similarly, no significant differences (p<0.05) were observed between Persian gum 6 percent (W/V), gum Arabic 6 percent (W/V), the combination of Persian gum 3 percent (W/V) and gum Arabic 3 percent (W/V), and skim milk 10 percent (W/V) in terms of their effects on the survival of Escherichia coli. Skim milk 10 percent (W/V) was, however, found to have significant (p<0.05) effects on the survival of Xanthomonas axonopodis and Saccharomyces cerevisiae. Statistically significant (p<0.05) effects were observed after 14 days of cold storage (4°C) by Persian gum 6 percent (W/V) on the survival of Escherichia coli and by gum Arabic 6 percent (W/V) on the survival of Xanthomonas axonopodis and Saccharomyces cerevisiae. It was concluded that protective agents could be replaced by Persian gum for its effect on the survival rate of Escherichia coli and by skim milk for its effects on the survival of Xanthomonas axonopodis and Saccharomyces cerevisiae. Persian gum 6 percent (W/V) seemed to be the best protective agent for Escherichia coli during 14 days of its storage as gum Arabic 6 percent (W/V) seemed to have the same performance for Xanthomonas axonopodis and Saccharomyces cerevisiae. Persian gum 1 percent (W/V) was also found an alternative protective agent for the freeze drying of Lactobacillus plantarum subsp. plantarum PTCC 1896.

As Iranian Persian gum is cheap due to its wide availability and seems to have effects similar to those of gum Arabic and skim milk at low concentrations, it may be considered a good candidate for industrial applications.

Purpose – This study investigates the sociodemographics, late entry to antiretroviral therapy (ART), and clinical markers associated with AIDS-related mortality.

Methodology/Approach – Applying retrospective cohort design, 960 medical records of people who died of AIDS, from October 2006 to December 2014, were accessed from the ART center at tertiary health care center of Ranchi (India).

Findings – With useable data from 889 medical records revealed that the majority of people who died of AIDS consisted of married males in the age group of 19–40 years who were truck drivers, migrant laborers, and of rural origins. The median survival period was below 3 months following the ART. Males and people on the pre-ART group had a shorter survival period than their counterparts. Early mortality was associated with lower CD4+ T cell counts, the third or fourth clinical stage, ambulatory or bed-ridden functional status, and poor medication adherence.

Research limitations/implications – This study was limited to the analysis of AIDS deceased people only; it did not compare the survival duration with living people on ART. The lower CD4+ T cell counts and medication adherence, being strong predictors of mortality, can be addressed to attain higher survival rates of people who have AIDS.

Originality/Value of Paper – This is the first study conducted in the tribal-populated region, covering a large sample of 889 cases. Unique findings of this study update the existing data on AIDS-related mortality.

This paper aims to evaluate the cellular injuries associated with spray‐drying of Lactobacillus rhamnosus GG (LGG) in trehalose/monosodium glutamate (MSG) media by means of flow cytometry measurements; and also whether, and to what extent, the probiotic remain stable and viable in food formulations.

Spray‐drying was applied in the production of trehalose‐based preparations containing LGG. To gain more insights on the cellular damages that must have occurred during drying, flow cytometric analysis was applied in combination with carboxyfluorescein diacetate (cFDA) and PI stains. Spray‐dried samples were observed by scanning electron microscopy (SEM). The storage stability of spray‐dried LGG was monitored in food samples over a period of time.

It was observed that during spray‐drying, 1.80×10⁹ CFU/ml viable counts, which were equivalent of 68.8 per cent cells, were recovered in trehalose matrices but on incorporating 12.5 g/l MSG as a carrier component, survival rates were significantly improved. Density plot analysis showed a higher degree of membrane damage in cells spray‐dried in trehalose without MSG. SEM revealed no difference in the shapes and surfaces of spray‐dried samples. Evaluation of the recovery rates of LGG, initial count of ∼10⁹ CFU/ml or g, at storage time intervals revealed a minimum level of ∼10⁵ CFU/ml in apple juice after 12 days and ∼10⁷ CFU/g in chocolate beverages after ten weeks.

The potential contribution of MSG as a carrier component with trehalose in preventing higher losses during spray‐drying and food storage is pointed out in this study.