Search results

This paper aims to Separation and sorting of biological cells is desirable in many applications for analyzing cell properties, such as disease diagnostics, drugs delivery, chemical processing and therapeutics.

Acoustic energy-based bioparticle separation is a simple, viable, bio-compatible and contact-less technique using, which can separate the bioparticles based on their density and size, with-out labeling the sample particles.

Conventionally available bioparticle separation techniques as fluorescence and immunomagnetic may cause a serious threat to the life of the cells due to various compatibility issues. Moreover, they also require an extra pre-processing labeling step. Contrarily, label-free separation can be considered as an alternative solution to the traditional bio-particle separation methods, due to their simpler operating principles and lower cost constraints. Acoustic based particle separation methods have captured a lot of attention among the other reported label-free particle separation techniques because of the numerous advantages it offers.

This study tries to briefly cover the developments of different acoustic-based particle separation techniques over the years. Unlike the conventional surveys on general bioparticles separation, this study is focused particularly on the acoustic-based particle separation. The study would provide a comprehensive guide for the future researchers especially working in the field of the acoustics, in studying and designing the acoustic-based particle separation techniques.

The study insights a brief theory of different types of acoustic waves and their interaction with the bioparticles is considered, followed by acoustic-based particle separation devices reported till the date. The integration of acoustic-based separation techniques with other methods and with each other is also discussed. Finally, all major aspects like the approach, and productivity, etc., of the adopted acoustic particle separation methods are sketched in this article.

Dynamic separator is an equipment having a rotor and static vanes and is used to separate solids from gas-solids flow based on size. Particle separation in a dynamic separator happens due to complex interchanges between multiple forces exerted in the separation zone. Currently, there is only limited knowledge concerning the working principles of separation. This paper aims to systematically study a dynamic separator using numerical models to get insights into particle separation.

The Lagrangian–Eulerian formulation is used to simulate gas-solid flow. Multiple frames of reference using stage interpolation are used to account for rotation. Periodic symmetry in the equipment is exploited to create a simplified numerical model. The predictions from the numerical model are compared against available experimental data.

The numerical results indicate that only when particle collision is included, the separation efficiency trend from the experiment is matched by numerical predictions. Further, it is shown that at the same range of rotor speeds where numerical results predict increased separation efficiency, the solid pressure due to particle collision also reaches its maximum value. The gas flow and particle behavior in the separator are explained in detail.

The importance of particle collision in separation is interesting because traditionally, particle separation is assumed to be influenced by three forces, namely, centrifugal force, drag force and gravity. The numerical results, however, point to the contribution by particle collision, in addition to the above three forces.

The purpose of this paper is to show how particle scale simulation of industrial particle flows using DEM (discrete element method) offers the opportunity for better understanding of the flow dynamics leading to improvements in equipment design and operation.

The paper explores the breadth of industrial applications that are now possible with a series of case studies.

The paper finds that the inclusion of cohesion, coupling to other physics such fluids, and its use in bubbly and reacting flows are becoming increasingly viable. Challenges remain in developing models that balance the depth of the physics with the computational expense that is affordable and in the development of measurement and characterization processes to provide this expanding array of input data required. Steadily increasing computer power has seen model sizes grow from thousands of particles to many millions over the last decade, which steadily increases the range of applications that can be modelled and the complexity of the physics that can be well represented.

The paper shows how better understanding of the flow dynamics leading to improvements in equipment design and operation can potentially lead to large increases in equipment and process efficiency, throughput and/or product quality. Industrial applications can be characterised as large, involving complex particulate behaviour in typically complex geometries. The critical importance of particle shape on the behaviour of granular systems is demonstrated. Shape needs to be adequately represented in order to obtain quantitative predictive accuracy for these systems.

The purpose of this paper is to design the geometrical structure of banana screen, and this study aims to improve the screen efficiency.

The discrete element method was used to simulate the sieving process of banana screen with the inclinations of decks improved by Fibonacci sequence. The effect of each deck along screen length on the screening efficiency of particles was studied in this paper.

The comparisons among three groups of industrial banana screens with five different consecutive Fibonacci numbers have been made, and the variations of fine particle ratio were also investigated. The results show that the banana screening improved by the 5 Fibonacci sequence, 3, 5, 8, 13 and 21, has the good screening performance in the simulation. It is found that the inclination degree of the first deck at the feed end is the key factor to determine screening efficiency of banana screens.

It is not possible to consider all real physical factors exactly in a virtual model. The experiment prototype of banana screen was used to simply verify the feasibility of the methodology.

This work is helpful for designing the geometric parameters of the curved screen and gives a broad perspective to improve the efficiency of banana screens.

This paper aims to present the capacitance characterization of tapered dielectrophoresis (DEP) microelectrodes as micro-electro-mechanical system sensor and actuator device. The application of DEP-on-a-chip (DOC) can be used to evaluate and correlate the capacitive sensing measurement at an actual position and end station of liquid suspended targeted particles by DEP force actuator manipulation.

The capability of both, sensing and manipulation was analysed based on capacitance changes corresponding to the particle positioning and stationing of the targeted particles at regions of interest. The mechanisms of DEP sensor and actuator, designed in DOC applications were energized by electric field of tapered DEP microelectrodes. The actual DEP forces behaviour has been also studied via quantitative analysis of capacitance measurement value and its correlation with qualitative analysis of positioning and stationing of targeted particles.

The significance of the present work is the ability of using tapered DEP microelectrodes in a closed mode system to simultaneously sense and vary the magnitude of manipulation.

The integration of DOC platform for contactless electrical-driven with selective detection and rapid manipulation can provide better efficiency in in situ selective biosensors or bio-detection and rapid bio-manipulation for DOC diagnostic and prognostic devices.

This study aims to investigate the fatigue crack propagation behavior of SiC particle-reinforced 2124 Al alloy composites under constant amplitude axial loading at a stress ratio of R = 0.1. For this purpose, it is performed experiments and comparatively analyze the results by producing 5, 10, 15 Vol.% SiCp-reinforced composites and unreinforced 2124 Al alloy billets with powder metallurgy (PM) production technique.

With the PM production technique, SiCp-reinforced composite and unreinforced 2124 Al alloy billets were produced at 5%, 10%, 15% volume ratios. After the produced billets were extruded and 5 mm thick plates were formed, tensile and fatigue crack propagation compact tensile (CT) samples were prepared. Optical microscope examinations were carried out to determine the microstructural properties of billet and samples. To determine the SiC particle–matrix interactions due to the composite microstructure, unlike the Al alloy, which affects the crack initiation life and crack propagation rate, detailed scanning electron microscopy (SEM) studies have been carried out.

Optical microscope examinations for the determination of the microstructural properties of billet and samples showed that although SiC particles were rarely clustered in the Al alloy matrix, they were generally homogeneously dispersed. Fatigue crack propagation rates were determined experimentally. While the highest crack initiation resistance was achieved at 5% SiC volume ratio, the slowest crack propagation rate in the stable crack propagation region was found in the unreinforced 2124 Al alloy. At volume ratios greater than 5%, the number of crack initiation cycles decreases and the propagation rate increases.

As a requirement of damage tolerance design, the fatigue crack propagation rate and fatigue behavior of materials to be used in high-tech vehicles such as aircraft structural parts should be well characterized. Therefore, safer use of these materials in critical structural parts becomes widespread. In this study, besides measuring fatigue crack propagation rates, the mechanisms causing crack acceleration or deceleration were determined by applying detailed SEM examinations.

The purpose of this paper is to visualize protein manipulation using dielectrophoresis (DEP) as a substantial perspective on being an effective protein analysis and biosensor method as DEP is able to be used as a means for manipulation, fractionation, pre-concentration and separation. This research aims to quantify DEP using an electrochemical technique known as cyclic voltammetry (CV), as albumin is non-visible without any fluorescent probe or dye.

The principles of DEP were generated by an electric field on tapered DEP microelectrodes. The principle of CV was analysed using different concentrations of albumin on a screen-printed carbon electrode. Using preliminary data from both DEP and CV methods as a future prospect for the integration of both techniques to do electrical quantification of DEP forces.

The size of the albumin is known to be 0.027 µm. Engineered polystyrene particle of size 0.05 µm was selected to mimic the DEP actuation of albumin. Positive DEP of the sample engineered polystyrene particle was able to be visualized clearly at 10 MHz supplied with 20 Vpp. However, negative DEP was not able to be visualized because of the limitation of the apparatus. However, albumin was not able to be visualized under the fluorescent microscope because of its translucent properties. Thus, a method of electrical quantification known as the CV technique is used. The detection of bovine serum albumin (BSA) using the CV method is successful. As the concentration of BSA increases, the peak current obtained from the voltammogram decreases. The peak current can be an indicator of DEP response as it correlates to the adsorption of the protein onto the electrodes. The importance of the results from both CV and DEP shows that the integration of both techniques is possible.

The integration of both methods could give rise to a new technique with precision to be implemented into the dialyzers used in renal haemodialysis treatment for manipulation and sensing of protein albumin.

The purpose of this study is to explore how particle shape influences the screening, including screening efficiency per unit time, and the relationship between vibration parameters and screening efficiency per unit time in discrete element method (DEM) numerical simulations.

In this paper, a three-dimensional discrete element model of vibrating screen with composite vibration form of swing and translation was proposed to simulate the screening process. In total, 11 kinds of non-spherical particles whose shapes changed in a continuous regularity gradual process were established using a multi-sphere method. In the DEM simulations, vibration parameters, including vibration frequency, vibration amplitude and stroke angle, and swing parameters, including swing frequency and swing angle, were changed to perform parametric studies.

It shows that the effect of particle shape on screening efficiency is quantitative actually. However, the trends of different shape particles’ screening efficiency per unit time are mainly consistent.

Some simple particle shapes can be expected to be explored to do screening simulation studies reasonably with modification of the simulation data in DEM numerical simulations. That may improve the computational efficiency of numerical simulations and provide guidance to the study of the screening process.

This paper aims to present the dielectrophoresis (DEP) force (FDEP), defined as microelectrofluidics mechanism capabilities in performing selective detection and rapid manipulation of blood components such as red blood cells (RBC) and platelets. The purpose of this investigation is to understand FDEP correlation to the variation of dynamic dielectric properties of cells under an applied voltage bias.

In this paper, tapered design DEP microelectrodes are used and explained. To perform the characterization and optimization by analysing the DEP polarization factor, the change in dynamic dielectric properties of blood components are observed according to the crossover frequency (f_xo) and adjustment frequency (f_adj) variation for selective detection and rapid manipulation.

Experimental observation of dynamic dielectric properties change shows clear correlation to DEP polarization factor when performing selective detection and rapid manipulation. These tapered DEP microelectrodes demonstrate an in situ DEP patterning efficiency more than 95%.

The capabilities of tapered DEP microelectrode devices are introduced in this paper. However, they are not yet mature in medical research studies for various purposes such as identifying cells and bio-molecules for detection, isolation and manipulation application. This is because of biological property variations that require further DEP characterization and optimization.

The introduction of microelectrofluidics using DEP microelectrodes operate by selective detecting and rapid manipulating via lateral and vertical forces. This can be implemented on precision health-care development for lab-on-a-chip application in microfluidic diagnostic and prognostic devices.

This study introduces a new concept to understand the dynamic dielectric properties change. This is useful for rapid, label free and precise methods to conduct selective detection and rapid manipulation of mixtures of RBC and platelets. Further, potential applications that can be considered are for protein, toxin, cancer cell and bacteria detections and manipulation. Implementation of tapered DEP microelectrodes can be used based on the understanding of dynamic dielectric properties of polarization factor analysis.

A least‐squares finite element analysis of viscous fluid flow together with a trajectory integration technique for tracers is formulated and provides a mechanism for investigating mixing. Tracer integration is carried out using an improved Heun predictor‐corrector. Results from our supporting numerical studies on the CRAY and Connection Machine (CM) closely resemble the patterns of mixing observed in experiments. A “box‐counting” scheme and other measures to characterize the level of mixing are developed and investigated. This measure is utilized in numerical experiments to determine an optimal forcing frequency for mixing by periodic boundary motion in a rectangular enclosure. Some details concerning the numerical schemes and vector‐parallel implementation are also included.