Search results

To investigate the effect of operation parameters and printing configuration on the manufacturability of moulds in the manufacture of tissue engineering scaffolds using a 3D printing system.

The scaffold moulds were built using proprietary biocompatible materials using a modified Solidscape T66 ink‐jet printing system. The manufacturability of biological scaffold moulds has been investigated in terms of resolution, accuracy, and minimum and maximum manufacturable features.

The results demonstrated that the 3D system used in this study is able to fabricate structures with high reproducibility and flexibility. It was found that thermal degradation of BioSupport material had an adverse effect on resolution and accuracy of moulds printed for scaffold manufacturing. The maximum features, including maximum length and height, are geometrical dimension and orientation dependent. The system could produce a longer and higher features when the mould was aligned perpendicular to the axis of the mill than that parallel to the axis of the mill. The bigger the cross‐sectional area, the longer/higher the manufacturable feature the machine can produce. The accuracy and resolution are attributed to the size of the molten droplet of BioBuild that caused local melting of the support layer and which partially diffused into the support layer.

The results provide a guide to the design and fabrication of precision scaffold for tissue engineering using biocompatible materials.

This paper describes a method and process to evaluate the manufacturability of a scaffold mould using 3D printing technique. The limits to mould design are established, it could be extended to other solid freeform fabrication systems for effective operation and precision control.

The purpose of this paper is to adopt rapid prototyping (RP) technology to fabricate self‐hardening calcium phosphate composite (CPC) scaffolds with a controlled internal channel network to facilitate nutrient supplying and cell growth using RP technique and investigate their in vitro performance.

Porous scaffolds should possess branched channels to ensure uniform cell feeding and even flow of culture medium to promote uniform cell attachment and growth. A new three dimensional (3D) flow channel structure has been designed based on conversation of energy and flow. The CPC scaffold possessing such a channel network was made by indirect solid free form fabrication. Negative model of scaffold was designed by Pro/E software and its epoxy resin mold was fabricated on a sterolithography apparatus and the CPC slurry was filled in these molds. After CPC was self hardened, the mold was baked. The mold was removed by pyrolysis and then the designed scaffold was obtained.

The sizes of the fabricated scaffolds were consistent with the designed. The average compressive strength of the scaffold is approximately 6.0 MPa. Computational fluid dynamics and perfusion culture results showed that such a 3D flow channel arrangement would lead to a more uniform distribution of flow and cells and good transportation of nutrients.

The size errors of fabricated scaffolds could not escape and perfusion methods were difficult to control.

The basic design concept presented showed great promise for use in bone tissue engineering and fabrication method enhanced the versatility of scaffold fabrication. The designed scaffold structure made it possible to keep integrality of the scaffold when direct observation cells inside the channel by scanning electron microscopy (SEM).

This paper aims to review the advances in additive manufactured (AM) scaffolds for bone tissue engineering (TE). A discussion on the state of the art and future trends of bone TE scaffolds have been done in terms of design, material and different AM technologies.

Different structural features and materials used for bone TE scaffolds are evaluated along with the discussion on the potential and limitations of different AM scaffolds. The latest research to improve the biocompatibility of the AM scaffolds is also discussed.

The discussion gives a clear understanding on the recent research trend in bone TE AM scaffolds.

The information available here would be useful for the researchers working on AM scaffolds to get a quick overview on the recent research trends and/or future direction to work on AM bone TE scaffolds.

To fabricate the self‐hardening calcium phosphate composite scaffolds with controlled internal pore architectures using rapid prototyping (RP) techniques and investigate their in vitro bone tissue engineering responses.

The three‐dimensionally interconnected pores in scaffolds can facilitate sufficient supply of blood, oxygen and nutrients for the ingrowth of bone cells, tissue regeneration, and vascularization. It is essential for bone tissue engineering to provide an accurate control over the scaffolds material, porosity, and internal pore architectures. Negative image of scaffold was designed and epoxy resin molds were fabricated on sterolithography apparatus. Calcium phosphate cement slurry was cast in these molds. After self‐hardening, the molds were removed by pyrolysis and the resulting scaffolds were obtained.

Eight scaffolds with 54.45 percent porosity were tested on an Instron machine. The average compressive strength measured was 5.8±0.8 Mpa. Cytotoxicity and cell proliferation studies were conducted with rabbit osteoblast. Results showed that these scaffolds were non‐toxic and displayed excellent cell growth during the 2 weeks of in vitro culture.

The resulting scaffolds inherited errors and defects from the molds, such as cracks and dimensional changes.

The present method enhances the versatility of scaffold fabrication by RP. It is capable of reproducibly fabricating scaffolds from a variety of biomaterials.

In recent years, selective laser sintering (SLS) has been used in the biomedical field, including building small‐scaled biomedical devices such as tissue engineering scaffolds and drug delivery devices. A compact adaptation system for the SLS is needed to obtain a more effective and efficient way of sintering small‐scale prototypes so as to reduce powder wastage. Limitations of available smaller‐scale adaptation devices include the need of additional electrical supplies for the device. The purpose of this paper is to report the development of such a system to be mounted at the SLS part bed without any additional energy supply.

The compact adaptation device works on the concept of transferring the motion of the SLS part bed onto the part bed of the compact adaptation device. The device is an integrated attachment that is fixed onto the building platform of the SLS. The gear system of the device lifts the powder supply bed at both sides of the device simultaneously when the part bed at the center of the device is lowered. To further increase powder saving, an improved powder delivery system named alternative supply mechanism (ASM) is mounted on top of the roller to be coupled together with the compact adaptation device.

Powder saving up to 6.5 times compared to using full build version of the Sinterstation 2500 has been achieved by using the compact adaptation device. Furthermore, powder wastage has been reduced by 84 percent when using the ASM compared to the compact adaptation device alone.

The paper demonstrates the development and viability of adaptation devices for SLS to significantly reduce powder consumption by using solely mechanical means to build small parts without using external power supply.

This paper aims to introduce selective vacuum manufacturing (SVM), a powder-based rapid prototyping (RP) technique, and the ongoing development to improve its capability to apply in temporary scaffold fabrication.

SVM employs a combination of sand casting and powder sintering process to construct a prototype layer by layer. A dense layer of support material is prepared and selectively removed to create a cavity where part material is filled and sintered to form a solid layer. In order for SVM to be considered for scaffold fabrication, besides preparing poly-lactic acid (PLA) for part material, support material preparation and process parameters identification have been studied. Redesigning of SVM machine to be more suitable for the real usage has also been presented.

Particle size of salt has been controlled, and its suitable composition with flour and water has been determined. Process parameters have been identified to scale down the size of scaffolds to meso-scale and to achieve mechanical requirement. Properties of fabricated scaffolds have been enhanced and can be used for soft tissue applications. A prototype of the medical SVM machine has been constructed and tested. An examination of scaffolds fabricated on this new machine also showed their qualification for soft tissue application.

Further study will be on conducting a direct cytotoxicity test to provide the evidence for tissue growth before the clinical usage, on continuing to scaling down the scaffold size, and on improving SVM to meet the requirement of hard tissue.

This simple, inexpensive RP technique demonstrates its viability for scaffold fabrication.

This paper aims to investigate the mechanical behavior of three-dimensional (3D) calcium sulfate porous structures created by a powder-based 3D printer. The effects of the binder-jetting and powder-spreading orientations on the microstructure of the specimens are studied. A micromechanical finite element model is also examined to predict the properties of the porous structures under the load.

The authors printed cylindrical porous and solid samples based on a predefined designed model to study the mechanical behavior of the prototypes. They investigated the effect of three main build bed orientations (x, y and z) on the mechanical behavior of solid and porous specimens fabricated in each direction then evaluated the micromechanical finite-element model for each direction. The strut fractures were analyzed by scanning electron microscopy, micro-computed tomography and the von Mises stress distribution.

Results showed that the orientation of powder spreading and binder jetting substantially influenced the mechanical behavior of the 3D-printed prototypes. The samples that were fabricated parallel to the applied load had higher compressive strength compared with those printed perpendicular to the load. The results of the finite element analysis agreed with the results of the experimental mechanical testing.

The mechanical behavior was studied for the material and the 3D-printing machine used in this research. If one were to use another material formulation or machine, the printing parameters would have to be set accordingly.

This work aimed to re-tune the control factors of an existing rapid prototyping process for the given machine. The authors achieved these goals without major changes in the already developed hardware and software architecture.

The results can be used as guidelines to set the printing parameters and a model to predict the mechanical properties of 3D-printed objects for the development of patient- and site-specific scaffolds.

The purpose of this paper is to investigate the mechanical properties and porosity relationships in fused deposition modelling (FDM) fabricated porous structures.

Porous structures of numerous build architectures aimed at tissue engineering (TE) application were fabricated using the FDM. The employment of FDM to fabricate these non‐random constructs offers many advantages over conventional scaffold fabrication techniques as patient specific scaffolds with well‐defined architectures and controllable pore sizes can be fabricated accurately and rapidly. There exist several FDM parameters that one needs to specify during the scaffold fabrication process. These parameters, which can be interdependent and exhibit varying effects on scaffold properties, were identified and examined using the design of experiment (DOE) approach. Essentially, the effects of five FDM process parameters, namely air gap, raster width, build orientation, build layer and build profile, on the porosity and mechanical properties of acrylonitrile‐butadienene‐styrene (ABS) scaffold structures with three‐dimensional interconnectivity were investigated in two designed experiments. Statistical analyses of the data were performed and the respective factors that have significant influence on the porosity and mechanical properties of the scaffolds were identified. The relationship between scaffold's mechanical properties and porosity was thereafter established empirically.

Models of TE scaffolds of numerous build architectures were successfully fabricated using different parameter settings on the FDM. The DOE approach determined air gap and raster width as the most significant parameters in affecting the porosity and mechanical properties of the ABS scaffold structures. The relationship between scaffolds' mechanical properties and porosity was determined to be logarithmic, with the best mechanical properties observed in scaffolds of low porosity.

The paper highlights how the application FDM to tissue scaffold application can overcome most of the limitations encountered in the conventional techniques.

Part quality inspection is playing a critical role in the metal additive manufacturing (AM) industry. It produces a part quality analysis report which can be adopted to further improve the overall part quality. However, the part quality inspection process puts heavy reliance on the engineer’s background and experience. This manual process suffers from both low efficiency and potential errors and, therefore, cannot meet the requirement of real-time detection. The purpose of this paper is to look into a deep neural network, Convolutional Neural Network (CNN), towards a robust method for online monitoring of AM parts.

The proposed online monitoring method relies on a deep CNN that takes a real metal AM part’s images as inputs and the part quality categories as network outputs. The authors validate the efficacy of the proposed methodology by recognizing the “beautiful-weld” category from material CoCrMo top surface images. The images of “beautiful-weld” parts that show even hatch lines and appropriate overlaps indicate a good quality of an AM part.

The classification accuracy of the developed method using limited information of a small local block of an image is 82 per cent. The classification accuracy using the full image and the ensemble of model outputs is 100 per cent.

A real-world data set of high resolution images of ASTM F75 I CoCrMo-based three-dimensional printed parts (Top surface images with magnification 63×) annotated with categories labels. Development of a CNN-based classification model for the supervised learning task of recognizing a “beautiful-weld” AM parts. The classification accuracy using the full image and the ensemble of model outputs is 100 per cent.

This study aims to provide an overview of rapid prototyping (RP) and shows the potential of this technology in the field of medicine as reported in various journals and proceedings. This review article also reports three case studies from open literature where RP and associated technology have been successfully implemented in the medical field.

Key publications from the past two decades have been reviewed.

This study concludes that use of RP-built medical model facilitates the three-dimensional visualization of anatomical part, improves the quality of preoperative planning and assists in the selection of optimal surgical approach and prosthetic implants. Additionally, this technology makes the previously manual operations much faster, accurate and cheaper. The outcome based on literature review and three case studies strongly suggests that RP technology might become part of a standard protocol in the medical sector in the near future.

The article is beneficial to study the influence of RP and associated technology in the field of medicine.