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This study aims to investigate the tendency that Malaysian consumers will choose the recombinant collagen-like protein (RCLP) from bacteria as an alternative source of collagen in their consumption and the determinants of the consumer acceptance.

Quantitative approach is adopted in the study. Descriptive analysis and logistic regression are used to analyze primary data collected from a survey.

The likelihood of consumers choosing RCLP as an alternative source of collagen is higher among elderly and females. The choice is significantly influenced by the idea that the collagen should support maqasid al-syariah, approved by national Islamic body, should clear on the ingredients used and could boost local economic activities in the future.

An ethical halal policy should be outlined as the guiding principles to the potential producers of RCLP.

An alternative source of collagen using bacteria as proposed is not yet being introduced in Malaysian industry, and the analysis on probability that consumers will accept this new sourced collagen product is using primary data from survey.

The purpose is to analyze the mechanical behavior of the arterial wall in the degraded region of the arterial wall and to determine the stress distribution, as an important factor for predicting the potential failure mechanisms in the wall. In fact, while the collagen fiber degradation process itself is not modeled, zones with reduced collagen fiber content (corresponding to the degradation process) are assumed. To do so, a local weakness in the media layer is considered by defining representative volume elements (RVEs) with different fiber collagen contents in the degraded area to investigate the mechanical response of the arterial wall.

A three-dimensional (3D) large strain hierarchical multiscale technique, based on the homogenization and genetic algorithm (GA), is utilized to numerically model collagen fiber degradation in a typical artery. Determination of material constants for the ground matrix and collagen fibers in the microscale level is performed by the GA. In order to investigate the mechanical degradation, two types of RVEs with different collagen contents in fibers are considered. Each RVE is divided into two parts of noncollagenous matrix and collagen fiber, and the part of collagen fiber is further divided into matrix and collagen fibrils.

The von Mises stress distributions on the inner and outer surfaces of the artery and the influence of collagen fiber degradation on thinning of the arterial wall in the degraded area are thoroughly studied. Comparing the maximum stress values on outer and inner surfaces in the degraded region shows that the inner surface is under higher stress states, which makes it more prone to failure. Furthermore, due to the weakness of the artery in the degraded area, it is concluded that the collagen fiber degradation considerably reduces the wall thickness in the degraded area, leading to an observable local inflation across the degraded artery.

Considering that little attention has been paid to multiscale numerical modeling of collagen fiber degradation, in this paper a 3D large strain hierarchical multiscale technique based on homogenization and GA methods is presented. Therefore, while the collagen fiber degradation process itself is not modeled in this study, zones with reduced collagen fiber content (corresponding to the degradation process) are assumed.

The sources of collagens and gelatins are recently being questioned because it sourced from porcine or non-halal slaughtered animals. There are also concerns regarding the transfer of diseases from animal sources of collagen to human users. This study aims to propose an alternative source of collagen using recombinant collagen-like protein (halal-based) and to analyze factors contribute to the probability of using this alternative source of collagen among consumers in Malaysia.

Survey data collected are tested empirically using statistical tools and logistic regression.

This study finds two important inferences. First, the probability consumers opt for the alternative source of collagen is significantly determined by age of consumers and the level of their incomes. The likelihood of the consumers to opt for this alternative source of collagen is higher among elderly and lower income consumers. Second, the probability of using this alternative source of collagen with the purpose to protect the marine life is higher among young and lower income consumers. Thus, marketing variations of a product using this alternative source of collagen that is targeted to high age bracket and low income group of consumers often sees as a good strategy as compared with a more generalized marketing strategy. The younger consumers are also among potential consumers whose concern is more on health without endangering marine species or biodiversity.

The scope of areas in this study is limited to Klang Valley (Selangor and Kuala Lumpur, Malaysia). The respondents are those staying and working in these areas regardless of citizenship and ethnicity.

This study is proposing a new and promising way for mass production of collagen using recombinant collagen-like protein. Collagen-like protein was found in bacteria, and it may represent an alternative biosynthetic collagen material which is expected to be an alternative of the existing sources which are non-halal and endanger biodiversity and ecosystem.

To study the rheological behavior of aqueous dispersions of collagen gelled media and to bring it into relation with the gel stiffness and microstructure.

Collagen gelled media were prepared at different concentrations and their stiffness was measured with dynamic elastic modulus (DEM) technique. The gelled media were reduced into dispersions of micelles and gel liquid phase and the apparent viscosity of these systems was investigated as a function of the gap thickness, the shear rate and the collagen concentration. The gap thickness was varied between 20 and 200 μm. The microstructure of the broken gelled media was finally explored with optical microscopy.

DEM analysis has given values of the collagen gel stiffness ranging between 3 × 10⁻⁴ and 3.3 × 10⁻³ MPa. It has been shown that apparent viscosity grows with the increase of the gap thickness from 2 to 5 Pa s at constant collagen concentration and applied shear rate (4 percent, 100 s⁻¹). A rise in the collagen concentration has produced an increase in the apparent viscosity of gelled media dispersions. Nevertheless, the phenomenological law does not obey previously proposed equations but follows a sigmoid modal. Shear‐thinning phenomenon has been observed when the shear rate is varied between 10 and 2,000 s⁻¹.

The gel breaking process, which may cause changes in the microstructure of the micelles and hence in the rheological behavior of the dispersions, should be characterized measuring the forces acting on the samples during the rupture.

The paper provides useful information on the rheological behavior of aqueous dispersions of collagen gelled media.

This study aims to assess the influence of partial and simultaneous substitution of fat and sodium by hydrolyzed collagen and mix of herbs (MH) in chicken hamburgers, on the physical properties and proximal composition.

Five formulations were developed: (1) HCON–without adding collagen and MH; (2) C25M25–adding 25% of collagen and 25% of MH; (3) C25M50–adding 25% of collagen and 50% of MH; (4) C50M25–adding 50% of collagen and 25% of MH and (5) C50M50–adding 50% of collagen and 50% of MH. Chicken hamburgers were analyzed by proximal composition, sodium content, collagen and color analysis. The influence of treatments on texture profile, cooking performance and lipid oxidation of chicken hamburger was also investigated.

There was reduction in lipid and sodium for modified formulations compared with the HCON. There was great influence for some parameters, such as luminosity, cooking performance and texture profile. The formulation C50M50 showed the best cooking performance when compared to the others.

The formulation adding 50% of collagen and 50% of MH showed the highest yield and water retention (WR), with the least reduction in diameter and shrinkage, as well as presenting the best indexes of the texture profile and the lowest oxidation index, being recommended as the best combination of fat and sodium replacement.

This paper aims to extract the Cr from chrome containing leather waste (CCLW) in order to develop composite at optimum casting parameters using RSM technique. Chrome containing leather wastes (CCLW) is one of the significant cause of pollution that is exhaled by the leather industries. One of the technique to address the problem of pollution that is created by CCLW is to recycle it and produce some fruitful results from it. This will not only minimize the levels of harmful emissions to some extent but also give some befitting results.

The current work is all about exploring the ways by which CCLW could be used as a reinforcing material with aluminum. In this work, alumina has been used as a secondary reinforcement particle together with CCLW as with the help of stir casting process. The parameters of stir casting have been optimized by using “Response Surface Methodology.”

To maximize the hardness and tensile strength the values of optimal input casting parameters as found by the experimental results (response surface methodology) are as follows: the pre-heating temperature of collagen and alumina must be 166 °C and 300 °C, respectively, while the wt.% of collagen and alumina present in the matrix must be 2.45% and 5% sequentially 180 s of stirring time.

The hardness of the finally tested composite is 67.12 BHN (approx) which has been enhanced by 52.54% as compared to the base material. Tensile strength of composite also enhanced about 18% with respect to base material developed at the optimum combination of casting parameters.

Population aging was a global trend, and the most obvious thing after aging was the change in skin appearance. Therefore, the active ingredients that delay skin aging were particularly noticed. Past studies had pointed out that Chinese herbal extracts can improve skin elasticity, reduce wrinkles and melanin precipitation. The purpose of this paper is to explore whether combining hydrolyzed collagen with Chinese herbal extracts can improve skin conditions and achieve anti-inflammatory effects.

Fifty subjects were randomly divided into collagen or placebo groups, and one bottle of collagen or placebo drink was used every day for four weeks, after which skin and inflammatory factors were tested.

In comparison with the baseline results, the skin parameters were improving after four-week intervention. In addition, the IL-6, IL-8, TNF-a were significantly decreased and tissue inhibitor matrix metalloproteinase 1 (TIMP-1) was increased after four-week hydrolyzed collagen intervention.

This study showed that hydrolyzed collagen combined with Chinese herbal extracts can improve the condition of the skin, and can also reduce inflammatory associated factors, thereby achieving anti-aging effects.

To propose a new numerical elastohydrodynamic (EHD) analysis method suited to cinematic pairs lubricated with aqueous dispersions of collagen gelled media.

In comparison with traditional lubricants, these media are characterized by an apparent viscosity that increases with film thickness. Hence, the Reynolds equation has been numerically solved taking into account the new rheological law. The apparent viscosity of 4 percent collagen gel dispersions, as measured at a fixed shear rate of 100 l/s, is in the range 2‐5 Pa s, when film thickness varies between 20 and 200 μm. These experimental data have been fitted using a power law. The proposed analysis method has been applied to a 180° partial bearing, which rigid journal rotates in a flexible sleeve, made up by a resin shell.

The results of this tribological analysis have been compared with those concerning traditional isoviscous fluids which viscosity has been set equal to the viscosity of the gelled media when the film thickness is constant, i.e. in the unloaded bearing. The minimum film thickness calculated has been turned out equal to 66.8 μm in the former case, and 27.6 μm in the latter case. Furthermore, when the cinematic pair is lubricated with the aqueous gel media, film thickness distribution over the bearing surface is more uniform, active film region is wider and, consequently, peak pressure is lower than when the isoviscous lubricant is employed.

For lightly loaded journal bearings, the dependence between apparent viscosity and the shear rate in the lubricant film has been neglected, so that the apparent viscosity has been considered changing only with the film thickness.

The paper introduces a new numerical EHD analysis method for use with cinematic pairs lubricated with aqueous dispersions of collagen gelled media.

The purpose of this paper is to fabricate and characterize osteochondral beta‐tricalcium phosphate/collagen scaffold with bio‐inspired design by ceramic stereolithography (CSL) and gel casting.

Histological analysis was applied to explore the morphological characteristics of the transitional structure between the bone and the cartilage. The acquired data were used to design biomimetic biphasic scaffolds, which include the bone phase, cartilage phase, and their transitional structure. The engineered scaffolds were fabricated from β‐TCP‐collagen by CSL and gel casting. The cartilage phase was added to the ceramic phase by gel‐casting and freeze drying.

The resulting ceramic scaffolds were composed of a bone phase with the following properties: 700‐900 μm pore size, 200‐500 μm interconnected pores size, 50‐65 percent porosity, fully interconnected, ∼12 Mpa compressive strength. A suitable binding force between cartilage phase and ceramic phase was achieved by physical locking that was created by the biomimetic transitional structure. Cellular evaluation showed satisfactory results.

This study is the first try to apply CSL to fabricate biological implants with β‐TCP and type‐I collagen. There are still some defects in the composition of the slurry and the fabrication process.

This strategy of osteochondral scaffold fabrication can be implemented to construct an osteochondral complex that is similar to native tissue.

The CSL technique is highly accurate, as well as biologically secure, when fabricating ceramic tissue engineering scaffolds and may be a promising method to construct hard tissue with delicate structures. The present strategy enhances the versatility of scaffold fabrication by RP.

Recently, powerful instruments for biomedical engineering research studies, including disease modeling, drug designing and nano-drug delivering, have been extremely investigated by researchers. Particularly, investigation in various microfluidics techniques and novel biomedical approaches for microfluidic-based substrate have progressed in recent years, and therefore, various cell culture platforms have been manufactured for these types of approaches. These microinstruments, known as tissue chip platforms, mimic in vivo living tissue and exhibit more physiologically similar vitro models of human tissues. Using lab-on-a-chip technologies in vitro cell culturing quickly caused in optimized systems of tissues compared to static culture. These chipsets prepare cell culture media to mimic physiological reactions and behaviors.

The authors used the application of lab chip instruments as a versatile tool for point of health-care (PHC) applications, and the authors applied a current progress in various platforms toward biochip DNA sensors as an alternative to the general bio electrochemical sensors. Basically, optical sensing is related to the intercalation between glass surfaces containing biomolecules with fluorescence and, subsequently, its reflected light that arises from the characteristics of the chemical agents. Recently, various techniques using optical fiber have progressed significantly, and researchers apply highlighted remarks and future perspectives of these kinds of platforms for PHC applications.

The authors assembled several microfluidic chips through cell culture and immune-fluorescent, as well as using microscopy measurement and image analysis for RNA sequencing. By this work, several chip assemblies were fabricated, and the application of the fluidic routing mechanism enables us to provide chip-to-chip communication with a variety of tissue-on-a-chip. By lab-on-a-chip techniques, the authors exhibited that coating the cell membrane via poly-dopamine and collagen was the best cell membrane coating due to the monolayer growth and differentiation of the cell types during the differentiation period. The authors found the artificial membrane, through coating with Collagen-A, has improved the growth of mouse podocytes cells-5 compared with the fibronectin-coated membrane.

The authors could distinguish the differences across the patient cohort when they used a collagen-coated microfluidic chip. For instance, von Willebrand factor, a blood glycoprotein that promotes hemostasis, can be identified and measured through these type-coated microfluidic chips.