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Biopigments, natural colors from microbiological origin are of great interest because of their potential advantages over synthetic colorants. Therefore, this paper aims to evaluate the best possible fermentative conditions for the maximum production of biopigment using solid state fermentation and submerged fermentation by Monascus purpureus MTCC 369.

The biopigment was produced using solid state fermentation and submerged with optimized substrate to achieve higher yield. The statistical analysis was carried out using a Microsoft Excel ® (Microsoft Corporation).

On comparative analysis, it was observed that solid state fermentation resulted significant accumulation of biopigment (9.0 CVU/g) on the 9th day in comparison to submerged fermentation (5.1 CVU/g) on the 15th day.

Results revealed that sweet potato peel powder and pea pods provides necessary nutrients required for mycelial growth, and biopigment production, therefore, can be used as potent substrate for biopigment production by Monascus purpureus MTCC 369. Extracted color can be used in confectionery, beverages and pharmaceutical industries.

This work focuses on utilisation of waste for production of pigment as alternative source to synthetic colorant, and few studies have been carried out using wastes, but no work has been carried out on sweet potato peel to the best of the authors’ knowledge.

This paper aims to extract colour from micro-organisms (as a source of natural pigments) using agro-industrial substrates to replace synthetic media by solid state fermentation. Nature is filled with colours. Due to health and environmental consciousness among people, use of synthetic colour has declined, and so the need to develop colour from cheap and easily available natural sources (plants, animals, micro-organisms and algae) using a cost-effective technique with higher yield and rapid growth. Monascus purpureus colour is a potent source of compounds (Dimerumic acid, Monacolin-k and -aminobutyric acid) having antimutagenic, antimicrobial and antiobesity, which helps in combating diseases.

Response surface methodology was used to optimise the biopigments extraction from Monascus purpureus using solid state fermentation.

The best optimised conditions for biopigments production using Monascus purpureus MTCC 369 were pH 5.4 at 32°C for 8 days 9 hours (8.9 days) from sweet potato peel and pea pod powder, 7.8 (w/w) and 3.9 per cent (w/w), respectively, which gave a final yield of 21 CVU/g. The model F-value of 69.18 and high value of adjusted determination coefficient 96.00 per cent implies high level of significance of the fitted model.

Extracted colour can be used in beverages, confectionery and pharmaceutical industries.

Colour produced using Monascus purpureus MTCC 369 is a natural source. As consumers are reluctant to use synthetic colour because of the undesirable allergic reactions caused by them, so a biopigment produced is natural colouring compound with wide application in food sector.

Selected sources of carbon and nitrogen were not used earlier by any researcher to extract biopigment from Monascus purpureus MTCC 369.

Monascus pigment was widely applied in food processing industry as functional additive, so more attention was paid to the fermentation optimization of pigment production. Therefore, this paper aims to evaluate the best possible fermentative conditions for maximum production of biopigment using submerged fermentation (SFM) and solid state fermentation (SSF) by Monascus purpureus HBSD 08.

The biopigment was produced by using an SMF and an SSF with optimized substrate to achieve higher yield. The antioxidant activity was evaluated by DPPH radical scavenging ability, superoxide anion radical scavenging ability and hydroxyl radical scavenging ability. The pigment composition was analyzed by thin layer chromatography.

Maximum Monascus pigment production (79.6 U/ml and 1,102 U/g) were obtained under an SFM and an SFF. The antioxidant activity of the pigment in an SFM was significantly higher than that in an SFM. The composition of pigment was not different in an SFM and an SFF.

The study developed new conditions, and Monascus strain was a candidate for producing pigment in an SFM and an SFF. To the authors’ best knowledge, this is a first attempt toward comparative evaluation on antioxidant capacity and composition between pigment in an SSF and an SFM. This result will serve for Monascus pigment production.

This paper aims to isolate fungal strains producing natural colours, explore their application as colourant in paints and develop cost-effective durable natural paints.

Fungal strains producing natural colours of different shades were isolated. Colourant production was carried out by fermentation method. Natural lime, milk, oil-in-water emulsion paints using natural microbial colours and eco-friendly ingredients were prepared. Bio-paint applications were carried out and evaluated.

Our results indicate that microbes in general and fungi in specific represent dependable source of variety of natural colours, and cost-effective durable natural paints can be prepared with commonly available natural ingredients using scientific information based on history of paints.

Natural colours are gaining importance because of their use in health, nutrition, pharmaceutical, textile and environmental applications. Nature is quite rich in several types of colourants. Chemical synthesis of synthetic dyes is complex and not environmental friendly. Microbial dyes manufactured can evade inherent environmental problems of synthetic dyes and offer significant opportunity as a colourant in paints. However, only generally regarded as safe microbial strains are to be considered for colour production.

Choosing natural alternatives to protect the health and environment is the need of hour. Fungal colourants are relatively more stable and robust and offer significant opportunity as a colourant in paints. Cost-effective durable natural paints can be prepared using selected stable fungal colourants with commonly available natural ingredients. High diversity of rich and complex natural colourants can be obtained from microorganisms. With the available techniques of fermentation, natural colours can be produced in large quantities of on an economically viable scale and explored for their applications.

Bio-paints are eco-friendly natural paints, low volatile organic compounds (VOC) paints or organic paints alternate to conventional paints. Most of these natural paints are durable, breathable, prevent moisture problems, contribute to a positive room climate, use safer technology and are less energy-intensive than conventional latex paints to produce. These paints improve indoor air quality and reduce urban smog and offer beneficial characteristics such as low odour, excellent durability and a washable finish.

Many of the old art works that still survive today are a tangible proof and evidence of beauty and durability of natural paints. Organic materials used in these paints include natural pigments of mineral, plant and animal origin and other raw biodegradable ingredients. Successful commercialisation of many microbial pigments for food and textile applications is reported in literature. Therefore, present research work aims at developing natural paints using microbial pigments and recipes that have been successfully used by people for years.

An upsurge in health and environmental concerns over the use of synthetic color has made the development of color from cheap and easily available natural sources, namely, plants, animals, micro-organisms and algae as indispensable. This study aims to extract anthocyanins, an important natural plant pigments, from Sohiong (Prunus nepalensis). This study demonstrated that Sohiong have high anthocyanins content and antioxidant property, indicating an immense potential for the fruit producers and food processors.

Response surface methodology was used to optimize the conditions for extraction of anthocyanins from Sohiong using conventional solvent extraction.

The optimum conditions for extraction were found to be 36.75°C temperature, 60.32 per cent ethanol concentration and 2.39 per cent citric acid concentration with recovery of 45 per cent total extract yield, 858.84 mg C3G/100g DM anthocyanin content and 824.91 mg GAE/100g DM phenolic content with in-vitro antioxidant activity of 31.40 mmol AEAC/100g DM for FRAP and 84.66 per cent DPPH scavenging capacity (20mg/ml). The F-values and high values of adjusted determination coefficient for each response imply high level of significance of the fitted models.

Extracted color can be used in food and pharmaceutical industries.

Pigment extracted is from a natural source and possesses high antioxidative activity and potential health benefits. With increasing demand for natural colors and other additives, there is a wide range of applications of the pigment as natural colorant in the food and pharmaceutical sector.

Selected plant source, i.e. Sohiong, was not used earlier by any researcher to extract anthocyanins for potential applications as food colorant.

The purpose of this study is to investigate the influence of light intensity and sources of carbon and nitrogen on the cultivation of Spirulina maxima.

Cultures were carried out in a modified Zarrouk medium using urea, sodium acetate and glycerol. A Taguchi experimental design was used to evaluate the effect on the production of biocompounds: productivities in biomass, carbohydrates, phycocyanin and biochar were analyzed.

Statistical data analysis revealed that light intensity and sodium acetate concentration were the most important factors, being significant in three of the four response variables studied. The highest productivities in biomass (46.94 mg.L⁻¹.d⁻¹), carbohydrates (6.11 mg.L⁻¹.d⁻¹), phycocyanin (3.62 mg.L⁻¹.d⁻¹) and biochar (22, 48 mg.L⁻¹.d⁻¹) were achieved in experiment 4 of the Taguchi matrix, highlighting as the ideal condition for the production of biomass, carbohydrates and phycocyanin.

Sodium acetate and urea can be considered, respectively, as potential sources of carbon and nitrogen to increase Spirulina maxima productivity. From the results, an optimized cultivation condition for the sustainable production of bioproducts was obtained.

This work focuses on the study of the influence of light intensity and the use of alternative sources of nitrogen and carbon on the growth of Spirulina maxima, as well as on the influence on the productivity of biomass and biocompounds. There are few studies in the literature focused on the phycocyanin production from microalgae, justifying the need to deepen the subject.

Internal fluffy portion along with fibrous strands of ripe pumpkin is considered as waste in processing industries though it contains sufficient amount of ß-carotene pigment. The purpose of this paper is to use the leftover fluffy portion of ripe pumpkin (Cucurbita maxima) after the use of its flesh for the purpose of processing.

The data were analyzed statistically by following a complete randomized design (CRD). All analysis were performed using the software OPSTAT.

One hour pre-enzymatic treatment before solvent extraction showed significant improvement in extraction yield in comparison to the isolation of ß-carotene pigment through solvent only. Temperature time combination was optimized as 40°C for 2 h during solvent extraction to obtain maximum yield irrespective of the type of extraction method used.

Extracted carotene pigment can further be used as a natural food colorant in processed food products not only to enhance the color appeal but also it improves the nutritional value of the product as ß-carotene acts as a precursor of vitamin A.

Coloring agents of natural origin are becoming famous among society due to their health benefits. Consumers are becoming reluctant to use synthetic colors because of the undesirable allergic reactions caused by them, so carotene bio-pigment produced is a natural coloring compound with wide application in the food sector.

Even though few researchers have worked on the extraction of carotene pigment from pumpkin, but no researcher has reported the use of a waste fluffy portion of C. maxima for extraction of ß-carotene pigment.

The Monascus pigment has been widely applied in the food processing industry as a functional additive. Lovastatin and polysaccharides are two important bio-active materials found in Monascus. Citrinin is considered as mycotoxin. Thus, it is important to produce high yields of intracellular Monascus pigments with high yields of lovastatin and polysaccharides, while maintaining low citrinin yields under liquid fermentation.

The intracellular yields of pigments, lovastatin, polysaccharides and citrinin; biomass; and reducing the sugar content of Monascus purpureus HBSD 08 were determined every day during a 10-day culturing period using lactose, maltose, sucrose, glucose, glycerine and xylose as the sole carbon sources. Additionally, the pigment composition was analysed by a thin layer chromatography (TLC) and the in vitro antitumor activities of the pigments were determined.

The maximal yield of pigments (55.44 U/mL after six days of culture) and lovastatin content (1,475.30 µg/L after five days of culture) were obtained in the presence of glucose and maltose as the sole carbon sources, respectively. The suitable carbon sources for high intracellular polysaccharides yields were sucrose, maltose and xylose. Glucose should not be chosen as the sole carbon source because of its high food safety risk. In vitro antitumor activities of pigments in the presence of different carbon sources were in the order of xylose > glucose = maltose > glycerine > sucrose = lactose. The pigment compositions in the presence of different carbon sources were the same from the TLC analysis. Thus, maltose displayed high intracellular yields of pigments, lovastatin and polysaccharides; high food safety against citrinin, and high in vitro antitumor activity during the ten days culturing period.

This study shows us the benefits of using maltose as a substrate in the production of intracellular Monascus pigments while ensuring economic and food safety.