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Five different structural reactive dyes (Reactive Brilliant Blue K-3R, Everacion Blue H-ERD, Moderzol Blue FBR, Atuzol Black B and Moderzol Blue HEGN) were treated with laccase (Denilite II US) in order to determine the optimum decolouration conditions. The experiments showed that laccase had distinct decolouration effects on these five dyes. Under optimum conditions, the colour removal rates of Everacion Blue H-ERD and Moderzol Blue HEGN were over 90%. Furthermore, the effects of different additives, such as acid ion, metal ion, and surfactants on the decolouration rate of Reactive Brilliant Blue K-3R were discussed. The results show that the decolouration rate is significantly promoted through the addition of Cu²⁺ and Al³⁺, while it is inactivated with Fe²⁺ and ion surfactants. Moreover, the COD removal rates of the five dyes are more than 75%.