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Fresh vegetables contain advantageous phytochemical components, making them one of the most significant sources of nutrition. The threat of harmful bacteria still exists because these vegetables are not heated in restaurants before being consumed. Therefore, this study aimed to evaluate the microbial quality of fresh vegetables in restaurants of different levels.

A total of 499 fresh vegetable samples (from sandwiches and fresh-cut vegetable salads) were collected from 3 different types of food service establishment: 201 from international restaurants (IRs), 210 from national restaurants (NRs), and 88 from cafeterias (CAs). The samples were prepared and inoculated on specific growth media. The aerobic mesophilic bacteria (AMB) Campylobacter spp., Staphylococcus aureus (S. aureus), Enterobacteriaceae, Escherichia coli (E. coli) and yeast and molds were counted, and Listeria monocytogenes, Salmonella spp. and Escherichia coli O157 were detected using specialized medium.

High counts of S. aureus, above 3 log cfu/g, suggested that 71.5% of samples collected from NRs and 77.3% from CA were not accepted, whereas 81.6% of samples collected from IRs were accepted. The low population of E. coli, less than 2 log cfu/g, suggested that 99.0, 97 and 92.0 % of samples collected from IRs, NRs and CA, respectively, were accepted. Listeria monocytogenes and Escherichia coli O157 were absent from every sample. One sample was positive for Salmonella spp. in each of the NR and CA sample groups.

RIs adhere to health and hygiene standards better than NRs and CAs, according to the findings of vegetable contamination tests.

The main purpose of this study resides essentially in the development of a new tool to quantify the biomass in the bioreactor operating under steady state conditions.

Modeling is the most relevant tool for understanding the functioning of some complex processes such as biological wastewater treatment. A steady state model equation of activated sludge model 1 (ASM1) was developed, especially for autotrophic biomass (XBA) and for oxygen uptake rate (OUR). Furthermore, a respirometric measurement, under steady state and endogenous conditions, was used as a new tool for quantifying the viable biomass concentration in the bioreactor.

The developed steady state equations simplified the sensitivity analysis and allowed the autotrophic biomass (XBA) quantification. Indeed, the XBA concentration was approximately 212 mg COD/L and 454 mgCOD/L for SRT, equal to 20 and 40 d, respectively. Under the steady state condition, monitoring of endogenous OUR permitted biomass quantification in the bioreactor. Comparing XBA obtained by the steady state equation and respirometric tool indicated a percentage deviation of about 3 to 13%. Modeling bioreactor using GPS-X showed an excellent agreement between simulation and experimental measurements concerning the XBA evolution.

These results confirmed the importance of respirometric measurements as a simple and available tool for quantifying biomass.

The purpose of this article is to provide information about interactions between pink-pigmented facultative methylotroph (PPFM) organisms and plants, their molecular mechanisms of methylotrophic metabolism, application of PPFMs in agriculture, biotechnology and bioremediation and also to explore lacuna in PPFMs research and direction for future research.

Research findings on PPFM organisms as potent plant growth promoting organisms are discussed in the light of reports published by various workers. Unexplored field of PPFM research are detected and their application as a new group of biofertilizer that also help host plants to overcome draught stress in poorly irrigated crop field is suggested.

PPFMs are used as plant growth promoters for improved crop yield, seed germination capacity, resistance against pathogens and tolerance against drought stress. Anti-oxidant and UV resistant properties of PPFM pigments protect the host plants from strong sunshine. PPFMs have excellent draught ameliorating capacity.

To meet the ever increasing world population, more and more barren, less irrigated land has to be utilized for agriculture and horticulture purpose and use of PPFM group of organisms due to their draught ameliorating properties in addition to their plant growth promoting characters will be extremely useful. PPFMs are also promising candidates for the production of various industrially and medicinally important enzymes and other value-added products. Wider application of this ecofriendly group of bacteria will reduce crop production cost thus improving economy of the farmers and will be a greener alternative of hazardous chemical fertilizers and fungicides.

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Buildings respond differently to microbial invasion depending on the design, type of construction materials and finishes used and extent of exposure to climatic factors. However, in the hot-humid tropical environment of Nigeria, much is not known about how buildings with different types of façade finishes or claddings are liable to microbial decay. The purpose of this research is to investigate the susceptibility of buildings with different types of façade finishes to microbial decay in Enugu metropolis, southeast Nigeria.

A survey involving physical observation of purposively selected 383 buildings and questionnaire administration to their owners was carried out in the study area. The data were subjected to descriptive and logistic regression analyses.

Most of the 383 buildings sampled were less than 41 year and 47% of them had painted façade finishes followed by 25.1% with cementitious finishes. Around 63.4% of the buildings had their façade finishes or claddings colonised by microbes. Older buildings of 15 years and above and those with cementitious materials and paints as their predominant façade finishes were more likely to experience microbial decay than newer ones and those having refractory bricks, ceramic tiles, aluminium composite materials and plastics/polymers as their predominant façade finishes or claddings.

The study identifies the categories of buildings that are likely to be more susceptible to microbial decay; and thus contributes to research on how to slow down the rate of biodeterioration of building façade finishes or claddings in the hot-humid tropical environments.

This is the first study on the susceptibility of buildings with different types of façade finishes or claddings to microbial decay in the hot-humid tropical environment of Enugu metropolis, southeast Nigeria. It also provides a clue on the age at which buildings become more vulnerable to microbial decay in the study area.

This research examines the association of physical development density, prevalence and types of microbes in colonized façade finishes of buildings in Enugu metropolis, Nigeria.

Survey and experimental research designs were adopted. A total of 383 buildings were investigated with samples collected from those with colonized façade finishes. The microbes were identified using the standard procedure for genomic sequencing with descriptive statistics, and the chi-square test used to analyse the data.

The results revealed a 64% prevalence of microbial colonization and a significant association between this and physical development density with 71.0% of the colonized buildings located in high-density neighbourhoods of the metropolis. The sequencing also showed 24 different microbes with Trichophyton tonsurans, Trichophyton mentagrophytes and Trichoderma harzianum species being the most common in the colonized façade finishes.

The research informs building professionals and owners of the specific microbes involved in the colonization of façade finishes of buildings in high-density urban areas. It also provides a clue about the nature of damages and defects associated with microbial colonization of building façades and the type of biocide additives required for the production of microbial-resistant façade finishes in the hot-humid tropical environment of Nigeria and beyond.

The study has shown that there is a significant relationship between the intensity of urban land use and microbial colonization of façade finishes of buildings. It also identified some new or less known microbes responsible for the biodeterioration of façade finishes and the effects this has on the buildings and public health in the hot-humid tropics of Enugu, Southeast Nigeria.

The purpose of this article is to investigate on changes of the microbial load and the chemical and physical properties of date fruits stored for 6 months under two different temperatures.

A composite sample of 100 kg date fruits from the Khalas variety, season 2019, was collected from the local market in Al Ahsa Province, Saudi Arabia, packaged in 1 kg lots, stored at room and refrigerator temperatures and the microbial contamination and the chemical and physical properties monitored over a period of six months of storage. Total bacteria, lactic acid bacteria, Enterobacteriaceae, yeasts and molds were counted and representatives of yeast and mold contaminants were identified using morphological, physiological and molecular typing techniques. Changes in the color and texture of the samples were also monitored during storage.

The yeasts detected were two strains of each of Lachancea thermotolerans and Rhodosporidiobolus fluvialis and one strain of Cystofilobasidium lacus-mascardii. For molds, one strain of each of Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum and Aspergillus caespitosus have been detected. No significant growth of these microorganisms was observed, but enough load persisted during storage that makes the samples not meeting the microbiological standards. There were significant changes in the color and texture of the fruits during storage.

These findings add important information that can help producers and processors to improve quality and promote marketing of date fruits, especially to international markets.

This paper aims to investigate the prepared modified alkyd and poly(ester-amide) (PEA) resins as antimicrobial and insecticide binders for surface coating applications.

Salicylic diethanolamine and 4-(N, N-dimethylamino) benzylidene glutamic acid were prepared and used as new sources of polyol and dibasic acid for PEA and alkyd resins, then confirmed by: acid value, FT-IR and 1H-NMR. The coating performance of the resins was determined using measurements of physico-mechanical properties. The biological and insecticide activities of the prepared resins were investigated.

The tests carried out revealed that the modified PEA and alkyd enhanced both phyisco-mechanical and chemical properties in addition to the biological and insecticide activities. The results of this paper illustrate that the introduction of salicylic diethanolamine and 4-(N, N-dimethylamino) benzylidene glutamic acid within the resin structure improved the film performance and enhanced the antimicrobial activity performance of PEA and alkyd resins.

The modified alkyd and PEA organic resins can be used as biocidal binders when incorporated into paint formulations for multiple surface applications, especially those that are exposed to several organisms.

Modified alkyd and PEA resins based on newly synthesized modifiers have a significant potential to be promising in the production and development of antimicrobial and insecticide paints, allowing them to function to restrict the spread of insects and microbial infection.

The purpose of the study was to produce a healthier, convenient and traditional ready-to-eat (RTE) snack option with increased nutritional value, using spent hen meat, dietary fibre (DF) and simple technological methods. The product was designed to be stable without refrigeration and be easily adoptable by local self-help groups, rural women and youth and entrepreneurs in urban and semi-urban areas.

Conventional binder used for making snacks, i.e. rice flour was partially replaced by different sources of antioxidant DFs, i.e. oat flour (T₁ – 10%), finger millet flour (T₂ – 5%) and amaranth flour (T₃ –15%) to prepare spent hen snack sticks (SHSS). The snacks were then packaged in low density polyethylene (LDPE) pouches and evaluated for their storage stability at ambient temperature for a period of 35 days. Their physico-chemical, sensory and microbiological quality was evaluated at a regular interval of 7 days. The proximate composition of developed SHSS was compared to commercially available snack products (chakli/murukku – snacks without meat).

The fibre-enriched SHSS showed significant improvement in nutritive value, as they contained more fibre (p = 0.001) and protein (p = 0.029) than control SHSS. When compared to commercially available snack product SHSS showed three-fold significant increase in protein (p = 0.000) and ash content (p = 0.001) and only 11%–12% total fat as compared to 31% fat in the market-available product. The most acceptable treatment in terms of overall sensory quality and nutritional aspects was T₃; however, T₂ was more shelf-stable during the storage period. The study showed that fibre-enriched snacks can be stored at ambient temperature for up to 35 days without substantial loss in physico-chemical, sensory and microbial quality. Hence, substituting rice flour with DFs can lead to the development of products with better sensory attributes and improved functionality.

The simplicity of the product in terms of composition, machinery and low production costs makes it an easily adoptable one by small-scale entrepreneurs, especially those belonging to semi-urban areas.

Incorporation of spent hen meat, a relatively cheap but abundant source of protein, in RTE products can serve as an effective way to alleviate protein malnutrition, whereas addition of fibre further improves the functionality of the product. The methodology can be easily taken up by small-scale entrepreneurs and create a market for snack-based functional meat products.

The purpose of this study is to present a systematic review of the effects of COVID-19 on the conventional textile production subsector. The emergence of the COVID-19 virus in 2019 has subsequently caused many problems, such as unemployment, business closures, economic instability and high volatility in the global capital markets amongst others within the wider manufacturing industry including textile production.

Relevant secondary data are obtained from the Scopus database and Statista. Based on the data analysis of 21 seed articles, three research themes are identified: challenges in the textile industry, new material innovations or solutions and the textile industry performance.

The results reveal that the COVID-19 pandemic has affected the textile industry, disrupted the supply chains of this industry, affected profit margins, stopped employment and impacted the retail of products to customers. Aside from the negative repercussions, there are also good sides to the pandemic which, for instance, range from advanced material innovations to textiles with anti-microbial, self-cleaning and anti-bacterial properties that would limit the transfer of the virus.

Findings reinforced the need for effective strategies and investments in the research and development departments of the various firms in the textile industry to innovate operations and novel materials for the next global pandemic.

Many companies have adopted novel strategies and practices that are helping them to survive the pandemic. This study, therefore, recommends further investigation into material innovations and reimagining strategies by companies and the supply chain within the textile industry so that it is protected against future crises.

This paper aims to examine the association between the dietary inflammatory index, the consumption of Enteral Nutrition Supplemented with probiotics with certain serum inflammation markers and gastrointestinal complications among individuals diagnosed with COVID-19.

This cross-sectional investigation involved 100 COVID-19 patients who were admitted to intensive care units in hospitals. These patients were administered two different types of Enteral Nutrition, so the dietary inflammatory index (DII), gastrointestinal complications and some serum inflammation markers have been compared between two groups.

The mean DII scores in all patients were significantly pro-inflammatory (probiotic formula 2.81 ± 0.01 vs usual formula group 2.93 ± 0.14 p = 0.19). The probiotic formula consumption had an inverse association with High-sensitivity C-reactive Protein concentration (coef = −3.19, 95% CI −1.25, −5.14 p = 0.001) and lead to a reduction of 2.14 mm/h in the serum level of Erythrocyte sedimentation rate compared to normal formula. The incidence of diarrhea, abdominal pain and vomiting in probiotic formula patients was respectively 94%, 14% and 86% less than in usual formula patients (p = 0.05).

In this cross-sectional study for the first time, the authors found that probiotic formula consumption was inversely associated with serum inflammation markers and gastrointestinal complications incidence. The high DII leads to more gastrointestinal complications incidence and inflammation markers. More studies are needed to prove this relationship.