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Purpose – The aim of this study is to determine the differences in the antimicrobial activity of katuk leaf (Sauropus androgynus (L.) Merr) against Escherichia coli.

Design/Methodology/Approach – The method used in this study was experimental posttest using a control group design. Analysis of the effect of katuk leaf was performed in the dilution method with 20%, 40%, 60%, 80%, and 100% concentration. The data were analyzed using one-way ANOVA test (α = 0.05) and was then tested using the least significant difference (LSD) test.

Findings – Bacterial colony counting that used total plant count found the average of E. coli amount at 20% of concentration (526.820 CFU/ml), 40% of concentration (449.380 CFU/ml), concentration of 60% (255.710 CFU/ml), concentration of 80% (194.110 CFU/ml), and at concentration 100% (168.600 CFU/ml). This study concluded that the katuk leaf extract at 20%, 40%, 60%, 80%, and 100% of concentration had antimicroba effect with significant influence. The 100% of concentration had the most significant effect compared with the other concentrations.

Research Limitations/Implications – Katuk leaf could be used as one of the alternative herbal choices that has a compound antimicrobial effect.

Originality/Value – This study increases the theoretical understanding of the difference of antimicrobial effectivity of katuk leaf extract (S. Androgynus (L.) Merr.) concentration against E. coli