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Current study was carried to detect the presence of pathogenic bacteria including the drug-resistant ones from milk and milk products. The paper aims to discuss these issues.

Twenty-six raw milk samples from ten different areas, 28 pasteurized milk samples from 12 different companies and 26 yogurt samples from ten different sources in Dhaka city were microbiologically analyzed through cultural and biochemical identification of the isolates. Drug resistance trait was also determined by the Kirby-Bauer method on Muller-Hinton agar.

Out of 80 samples studied, 74 were found to harbor pathogens within a range of 102-104 cfu/ml, including Escherichia coli, Salmonella spp., Staphylococcus aureus, and Vibrio spp. The study of antibiogram revealed that most of the isolates were resistant against most of the commonly used antibiotics.

Employment of only cultural/ biochemical tests excluding the molecular detection of virulence and/or antibiotic resistance genes might stand as a shortfall of the study. Nevertheless, such basic approach of microbiology can make this type of study replicable in the resource poor settings in the other developing countries.

Routine detection of drug-resistant bacteria can further unveil the complications in chemotherapy during the endemic food borne diseases.

The study outcome/knowledge would aid to a better public health management especially in the developing countries.

The presence of drug-resistant pathogenic bacteria in most of the tested milk samples poses a great public health threat, especially to the children. Therefore, the study revealed the necessity of maintaining proper hygienic practice and care in handling and processing of milk and milk products.

The purpose of the paper is to emphasize on contamination sources of freshly cultivated vegetables commonly consumed by the Bangladeshi people. Several local studies have been conducted to detect the microbial contamination within fresh vegetables, plantation lands and the irrigation waters separately; however, the correlation of microbial contamination between the fresh produces and the surrounding environment has not been clarified.

Bottle gourd (Lagenaria siceraria), pumpkin (Cucurbita pepo), radish (Raphanus sativus) and eggplant (Solanum melongena); their plantations soils and the fertilizers applied across the agricultural lands; and, finally, the irrigation waters used were analyzed from nine districts of Bangladesh using conventional microbiological and biochemical methods.

Almost all vegetable samples studied were found to be immensely contaminated with bacteria and fungi. Among the pathogens, Klebsiella spp., Staphylococcus spp. and Pseudomonas spp. were found to be dominant. Besides, massive microbial growth was also observed in the plantation soils and fertilizers, including Klebsiella spp., Pseudomonas spp., Bacillus spp., Listeria spp., Escherichia coli and Vibrio spp. Existence of the fecal coliforms, E. coli, Klebsiella spp., Salmonella spp. and Listeria spp., was noticed in the irrigation waters.

Although the present study revealed the combined results connecting the vegetable contamination aspect with the knowledge on microbiology ultimately in the food chain, implementation of molecular studies detecting the virulence genes both in the fresh produces and the plantation soils, fertilizers and the irrigation waters would further clarify the microbial dissemination mechanism.

Earlier studies demonstrated the ability of water bodies to disseminate numerous microorganisms into the plantation soils, and to some extent unraveled the ability of organic fertilizers to propagate pathogenic bacteria into the vegetation objects. These microorganisms may pose as a threat to vegetables, particularly by limiting crop production as well as the shelf life of the fresh produces.

The scenario of microbial divergence not only in the vegetables but also within the surroundings is gradually being heightened in Bangladesh principally due to the malpractice of sanitation, dumping the agricultural lands with feces, improperly controlled septic systems, waste water runoff across the agricultural lands, etc. Therefore, the preliminary and replicable experimental approach described in the current study would be feasible for all other developing countries to maintain the public health safety.

Growth and proliferation of microorganisms both in the vegetable samples and the environmental samples nearly to a similar extent indeed projected for the first time in Bangladesh, the agricultural perspective of the contamination sources of vegetables. Such knowledge would aid in the existing knowledge on the hygienic processing during crop production and harvesting for the sake of better consumer safety management.

With a previous throughput of sea fish contamination with microorganisms, the present study extended the array of such spoilage over four other fish samples including Pseudapocryptes elongates, Scomberomorus cavalla, Xenentodon cancila and Otolithoides pama, evaluated the reductive impact of irradiation, and further validated the irradiation methodology in controlling the microbial quality of the sea fish samples. The paper aims to discuss these issues.

Twelve samples of each sea fish were collected from super shops in Dhaka city and a portion of each sample was subjected to γ-irradiation at a dose of 3 kilo gray (kGy). Then, both non-irradiated and irradiated samples were tested for the presence of pathogenic bacteria though culture on different specific media followed by biochemical identification. Drug resistance among the pathogens was also investigated.

Most of the non-irradiated samples were observed to harbor huge bacteria and fungi (1.3×102-1.5×107 cfu/g or cfu/ml) including the fecal coliforms ranging up to 105 cfu/g or cfu/ml, leading to an elevated threat to public health. Besides, the isolates were found to be resistant against single or multiple antibiotics, which further brought treatment complications during the possible disease outbreaks. However, the pathogenic load was significantly reduced after applying 3 kGy dose of γ-irradiation on the samples in consistent to the previous work using different fish samples.

A similar type of work has already been published by the group using different samples this year in the Journal of Microbiology, Biotechnology and Food Sciences, Vol. 2 No. 4, pp. 2420-2430. However, even being an increment of the previous work, the present work deals with extended array of sea fish samples with an objective of controlling food safety.

The present work further confirms and assists the knowledge of food protection and the identification of spoiling bacteria and fungi by applying replicable methods projects of the novelty and practical outcome of the work.

The reduction of pathogenic load revealed the efficacy of γ-irradiation as a mean of preserving fish quality. Besides, the study quantified the contaminating microorganisms as well as identified the drug-resistant bacteria among sea fish samples. Traditional but standard cultural and biochemical tests, demonstration of drug resistance among the isolated microorganisms from fish samples and finally the microbial elimination by irradiation might contribute to the existing knowledge on major sea fish.