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This paper first analyses the direct effect of quality management practices (QMPs) on organisational ambidexterity (OA). It then analyses the mediating influence of the values of ambidextrous culture (AC). A novel theoretical model is tested in a quantitative study of the organic agri-food sector.

Quantitative methods and an online survey were used to collect and analyse 239 valid questionnaire responses. The data analysis method was partial least squares (PLS).

There is a relationship between QMPs and OA. AC partially mediates this link, strengthening the direct relationship between the two variables.

The proposed model is novel and creates opportunities for new lines of research. The concept of QMPs is considered in all its dimensions (processes, people, customers and suppliers). The study reaffirms the importance of the novel concept of AC in the literature. In this study, OA is defined in terms of the balanced processes of exploitation and exploration. The choice of sector is another original feature of the study.