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1 – 2 of 2Fouad Al Bayaty, Mazen M. Jamil Al-Obaidi, Anissa Lokman, Suhaila Yazid and Omar Emad Ibrahim
This study examines the osteoconductive and healing capabilities of locally implanted synthetic hydroxyapatite (sHAp) derived from eggshells in the central incisor sockets of rats.
Abstract
Purpose
This study examines the osteoconductive and healing capabilities of locally implanted synthetic hydroxyapatite (sHAp) derived from eggshells in the central incisor sockets of rats.
Design/methodology/approach
Toxicity experiments were conducted in vitro and in vivo, to testify the safety dosage of sHAp. Around 24 mature male Sprague–Dawley (SD) rats had their upper central incisors extracted. The rats were placed into three groups of eight rats each: Group 1: the sockets of extracted central incisors were left unfilled (control), Group 2: filled up with commercially available hydroxyapatite (HAp) and Group 3: implanted with sHAp locally retrieved from eggshells. After extraction, four rats from each group were sacrificed at 2nd and 4th weeks. Maxillary tissue sections were obtained and stained with hematoxylin and eosin (H&E) and Masson’s trichome (MT) staining. Anti-osteocalcin (OCN) and proliferating cell nuclear antigen (PCNA) were used primary antibodies for immunohistochemistry (IHC) special labeling.
Findings
The results showed that the locally implanted sHAp was non-toxic and safe in cell lines (human osteoblast and fibroblast) and animals. Histological analysis of H&E, MT and IHC showed that the sockets treated with locally implanted sHAp from eggshells were filled with new bone tissue of comparable thickness to other groups.
Originality/value
This unique technique uses locally implanted eggshell-derived sHAp with osteoconductive characteristics. In an in vivo model, sHAps increased OCN and PCNA expression to improve bone repair.
Details
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Adewale Allen Sokan-Adeaga, Godson R.E.E. Ana, Abel Olajide Olorunnisola, Micheal Ayodeji Sokan-Adeaga, Hridoy Roy, Md Sumon Reza and Md. Shahinoor Islam
This study aims to assess the effect of water variation on bioethanol production from cassava peels (CP) using Saccharomyces cerevisiae yeast as the ethanologenic agent.
Abstract
Purpose
This study aims to assess the effect of water variation on bioethanol production from cassava peels (CP) using Saccharomyces cerevisiae yeast as the ethanologenic agent.
Design/methodology/approach
The milled CP was divided into three treatment groups in a small-scale flask experiment where each 20 g CP was subjected to two-stage hydrolysis. Different amount of water was added to the fermentation process of CP. The fermented samples were collected every 24 h for various analyses.
Findings
The results of the fermentation revealed that the highest ethanol productivity and fermentation efficiency was obtained at 17.38 ± 0.30% and 0.139 ± 0.003 gL−1 h−1. The study affirmed that ethanol production was increased for the addition of water up to 35% for the CP hydrolysate process.
Practical implications
The finding of this study demonstrates that S. cerevisiae is the key player in industrial ethanol production among a variety of yeasts that produce ethanol through sugar fermentation. In order to design truly sustainable processes, it should be expanded to include a thorough analysis and the gradual scaling-up of this process to an industrial level.
Originality/value
This paper is an original research work dealing with bioethanol production from CP using S. cerevisiae microbe.
Highlights
Hydrolysis of cassava peels using 13.1 M H2SO4 at 100 oC for 110 min gave high Glucose productivity
Highest ethanol production was obtained at 72 h of fermentation using Saccharomyces cerevisiae
Optimal bioethanol concentration and yield were obtained at a hydration level of 35% agitation
Highest ethanol productivity and fermentation efficiency were 17.3%, 0.139 g.L−1.h−1
Hydrolysis of cassava peels using 13.1 M H2SO4 at 100 oC for 110 min gave high Glucose productivity
Highest ethanol production was obtained at 72 h of fermentation using Saccharomyces cerevisiae
Optimal bioethanol concentration and yield were obtained at a hydration level of 35% agitation
Highest ethanol productivity and fermentation efficiency were 17.3%, 0.139 g.L−1.h−1
Details