Multi-sensor integration on one microfluidics chip for single-stranded DNA detection
ISSN: 0260-2288
Article publication date: 16 September 2024
Issue publication date: 20 November 2024
Abstract
Purpose
This study aims to improve detection efficiency of fluorescence biosensor or a graphene field-effect transistor biosensor. Graphene field-effect transistor biosensing and fluorescent biosensing were integrated and combined with magnetic nanoparticles to construct a multi-sensor integrated microfluidic biochip for detecting single-stranded DNA. Multi-sensor integrated biochip demonstrated higher detection reliability for a single target and could simultaneously detect different targets.
Design/methodology/approach
In this study, the authors integrated graphene field-effect transistor biosensing and fluorescent biosensing, combined with magnetic nanoparticles, to fabricate a multi-sensor integrated microfluidic biochip for the detection of single-stranded deoxyribonucleic acid (DNA). Graphene films synthesized through chemical vapor deposition were transferred onto a glass substrate featuring two indium tin oxide electrodes, thus establishing conductive channels for the graphene field-effect transistor. Using π-π stacking, 1-pyrenebutanoic acid succinimidyl ester was immobilized onto the graphene film to serve as a medium for anchoring the probe aptamer. The fluorophore-labeled target DNA subsequently underwent hybridization with the probe aptamer, thereby forming a fluorescence detection channel.
Findings
This paper presents a novel approach using three channels of light, electricity and magnetism for the detection of single-stranded DNA, accompanied by the design of a microfluidic detection platform integrating biosensor chips. Remarkably, the detection limit achieved is 10 pm, with an impressively low relative standard deviation of 1.007%.
Originality/value
By detecting target DNA, the photo-electro-magnetic multi-sensor graphene field-effect transistor biosensor not only enhances the reliability and efficiency of detection but also exhibits additional advantages such as compact size, affordability, portability and straightforward automation. Real-time display of detection outcomes on the host facilitates a deeper comprehension of biochemical reaction dynamics. Moreover, besides detecting the same target, the sensor can also identify diverse targets, primarily leveraging the penetrative and noninvasive nature of light.
Keywords
Acknowledgements
Credit authorship contribution statement: All authors have read and agreed to the published version of the manuscript. Yuwei Cao, Data curation, Writing-original draft. Shuqi Xie, Methodology, Software, Writing – Original draft. Yue Ding, Visualization, Investigation. Kangning Cheng, Validation. Cong Liu, Conceptualization, Resources. Yanjing Ding, Investigation, Formal analysis. Xiaofeng Zhu, Writing-review and Editing. Huanqing Liu, Writing – Review and Editing. Muhammad Shafi, Writing – Review and Editing, Weiwei Yue, Conceptualization, Funding acquisition, Supervision Writing – Review and Editing.
Declaration of competing interest: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Data availability statement: Data will be made available on request.
The study was supported by the Natural Science Foundation of Shandong Province (Grant No. ZR2019MF025) and the Foundation of Shandong Provincial Key Laboratory of Biophysics (Grant No. SD2019BP003).
Citation
Yue, W., Cao, Y., Xie, S., Cheng, K.N., Ding, Y., Liu, C., Ding, Y.J., Zhu, X., Liu, H. and Shafi, M. (2024), "Multi-sensor integration on one microfluidics chip for single-stranded DNA detection", Sensor Review, Vol. 44 No. 6, pp. 669-681. https://doi.org/10.1108/SR-06-2024-0562
Publisher
:Emerald Publishing Limited
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