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Introduction: The ethical implications of deoxyribonucleic acid (DNA) profiling or DNA fingerprinting or forensic genetics in criminal investigations have gained significant attention worldwide. In India, DNA profiling in criminal investigations has increased over the years. However, the ethical considerations of DNA profiling in India have yet to be examined adequately.

Purpose: The study aimed to examine the ethical considerations of DNA profiling in India and compare them with international guidelines. By examining the ethical considerations of DNA profiling in India, this study seeks to contribute to the ongoing discourse on the responsible use of DNA profiling in forensic investigations.

Methodology: The study used a qualitative research design, and data were collected by reviewing relevant literature and laws.

Findings: The findings indicate that the Indian legal framework has gaps in addressing the ethical considerations raised by international guidelines, such as the admissibility of DNA evidence in court, oversight of DNA laboratories, safeguards against discrimination, and privacy and confidentiality protections.

The comparative analysis highlights the need for strengthening the legal framework in India, adopting best practices from international guidelines, and incorporating safeguards to protect against discrimination and ensure the privacy and confidentiality of individuals. By adopting these recommendations, India can ensure that DNA profiling is conducted ethically and responsibly, promoting public trust in the criminal justice system and upholding the rights of all individuals.

A system that began with DNA tagging of oil cargoes has expanded to provide tracers for such diverse commodities as grain, silk, antiques, diamonds, high value documents and packaging. It has been used for a great variety of purposes. One is the covert tracing of marked commodities that have entered the country as “grey” imports, sold at reduced prices. Other users are interested in authenticating products, particularly in the antiques trade. Authentication of documents can be achieved through the incorporation of a DNA tracer into the ink used to print the document. It would be impossible for counterfeiters to determine the sequence of the added DNA marker, particularly in the presence of other masking DNA. The number of unique tags that can be provided by a DNA molecule is of the order of 10⁶⁰. Each application is unique and requires close collaboration between the company and the customer. It needs its own customized application and recovery procedure.

Organizational change is a key mechanism to ensure the sustainability of healthcare systems. However, healthcare organizations are persistently difficult to change, and literature is riddled with examples of failed change endeavors. In this chapter, we attempt to unravel the underlying causes for failed organizational change. We distinguish three types of change with different levels of depth that require different change approaches. Transformations are the deepest forms of change where beliefs and principles need to be modified to successfully influence routines. Renewals are deep forms of change where principles need to be modified to successfully influence routines. Improvements are shallow forms of change where only modifications at the level of routines are needed. Using deoxyribonucleic acid (DNA) as our metaphor, we propose a theory of “organizational DNA” to understand organizations and these three types of organizational changes. We posit that organizations are made up of a double helix consisting of a so-called “social string,” which contains the “soft” interaction or communication among the organization's members, and a so-called “technical string,” which contains “hard” organizational aspects such as structure and technology. Ladders of organizational nucleotides (i.e., Routines, Principles, and Beliefs) connect this double helix in various combinations. Together, the double helix and accompanying nucleotides make up the DNA of an organization. Without knowledge of the architecture of organizational DNA and whether a change addresses beliefs, principles, and/or routines, we believe that organizational change is constrained and based on luck rather than change management expertise. Following this metaphor, we show that organizational change fails when it attempts to change one part of the DNA (e.g., routines) in a way that renders it incompatible with the connecting components (e.g., principles and beliefs). We discuss how the theory can be applied in practice using an exemplar case.

Arthur W. Frank's dialogical narrative analysis (DNA) has been a recent addition to the plethora of methods in analysing stories. What makes this method unique from the rest is its concern for both the story's content and its effects. Stories are seen as selection/evaluation systems that do things for and on people. This chapter aims to provide the reader a heuristic guide in conducting DNA and emphasises learning through exemplars as the way of learning DNA. It provides an outline of DNA and reviews how researchers have applied it in different disciplines. Then, DNA will be applied in in the current ‘war on drugs’ in the Philippines. The stories of the policy actors – for and against the drug war – will be analysed to explore how stories affect policy choices and actions, call actors to assume different identities, associate/dissociate these actors and show how they hold their own in telling their stories. Finally, the potential of using DNA in criminology and criminal justice will be discussed.

Piperidine side chain-functionalized N, N′-bissalicylidene phenylene di amine di-anion (salphen) consisting of salphen-Zn and salphen-Cu are able to intercalate with nucleic base stacking of DNA and can be applied as an optical DNA hybridization detector. Attaching DNA and salphen to glass surfaces has been done via coating the surface with the silane coupling agents containing 3-aminopropyltriethoxysilane that was synthesized for acting as a high-affinity RNA carrier matrix. The Schiff base salphen-zinc (II) and salphen-Cu (II) complexes-labelled probe to target nucleic acid renders a colour change of the DNA biosensor to a green and red background colour for zinc and copper, respectively. This study aims to indicate that the DNA biosensor data with high efficiency is used for detection of dengue virus serotypes 2 (DENV-2) and Chikungunya virus (CHIKV) concentration via salphen-Zn (II) and salphen-Cu (II), respectively, in human samples.

¹H-NMR and ¹³C-NMR have been used via PerkinElmer LAMBDA 35 instrument. The authors also used a double beam spectrophotometer with (CH₃)4Si (TMS) as reference and dimethyl sulfoxide as solvent reference in pH = 7.0. Various DNA concentrations have been used for UV spectrophotometry at 300 nm and 400 nm for zinc and copper complexes, respectively. BRUKER mass spectra with DIONEX Ultimate 3000 LC model were used for all measurements. Mettler Teledo model (DSC882e) of differential scanning calorimeter (DSC) was used for measure the melting temperature of metal zinc and copper complexes. The morphology of the silica Nano spheres (SiNs) were scanned by FESEM with Model JSM-6700F from Japan.

The Cu (II) and Zn (II)-salphen-viruses DNA system for CHIKV and DENV-2, respectively, in different concentration have been investigated via various spectroscopies (Figure 3). CHIKV and DENV-2 DNA were selected from human saliva and urine samples as models for conformations of human G4-DNA. By increasing the amounts of DNAs, and G4, the UV–Vis bands of located above 300 nm, experienced a hypochromic effect. The Cu2+ complex exhibits selectivity towards the G4, and there is a similar affinity for Zn²⁺ complex binds to the G4. These results collectively suggest that the Cu²⁺ complex is stronger than the Zn2+ complex. The authors have found copper (II) and zinc (II) compounds and nucleic acid-complexes are strongly fluorescent molecules in the low energy range, from the visible to the near-infrared. Since the fluorescent emission of Zn (II) and Cu (II) complexes are enhanced by the binding to nucleic acids upon visible light exposure when bound to DNA. These complexes are important as selective fluorescent probes for nucleic acids and to highlight their potential application. UV–vis spectroscopy is an accurate for finding the extent of ligand interaction with DNA and metallic complexes–DNA binding. Generally, the binding of intercalative compounds to DNA can be characterized through absorption spectral titrations, where lowering in absorbance (hypochromism) and shift to longer wavelengths (red shift) were observed in this work.

The serum samples have been provided as citrate and collected in tubes after blood is allowed to clot. Then, it has been separated by centrifugation, and the authors have kept serum refrigerated at 4°C or frozen at –20°C. It is notable; specimens have been confirmed by Centres for Disease Control (CDC)-Dengue Branch previously. For the work, these samples have been frozen previously, and the diagnostic practiced tests at the CDC-Dengue Branch have been validated in serum and plasma. Therefore, plasma separated in lavender or heparins are suitable and acceptable for serology testing.

Neglected tropical diseases (NTDs) are a set of infectious diseases that primarily affect low-income countries situated near the equator. Effective diagnostic tools hold the key to stemming the spread of these infectious diseases. However, specificity is a major concern associated with current diagnostic protocols. In this regard, electrochemical deoxyribonucleic acid (DNA) biosensors could play a crucial role, as highlighted by renewed interest in their research. The purpose of this study was to highlight the current scenario for the design and development of biosensors for the detection of NTDs related pathogens. This review highlights the different types of factors involved and the modifications used to enhance sensor properties.

The authors discuss the potential of electrochemical DNA biosensors as efficient, affordable diagnostic tools for the detection of pathogens associated with NTDs by reviewing available literature. This study discusses the biosensor components, mainly the probe selection and type of electrodes used, and their potential to improve the overall design of the biosensor. Further, this study analyses the different nanomaterials used in NTD-based electrochemical DNA biosensors and discusses how their incorporation could improve the overall sensitivity and specificity of the biosensor design. Finally, this study examines the impact such techniques could have in the future on mass screening of NTDs.

The findings provide an in-depth analysis of electrochemical DNA biosensors for the detection of pathogens associated with NTDs.

This review provides an update on the different types and modifications of DNA biosensors that have been designed for the diagnosis of NTD-related pathogens.

The purpose of this paper is to compare the stability of the three nanocarriers created by DNA origami method with different positions and numbers of crossovers

Nanocarriers are attractive components among a variety of nanostructures created by DNA origami and can have numerous applications in mechanical and medical engineering. For this reason, the current study compares three nanotubes with different positions and numbers of crossovers created by DNA origami method that can be utilized as nanocarriers. To investigate the structures, the DNA nanocarriers are studied at the human body temperature 310 K. Molecular dynamics simulations are used for this study. For a quantitative analysis of DNA nanocarriers, the areas of three hexagons at three different sites in each of the nanotubes are investigated. The results indicate that the number and position of crossovers are among the significant factors in the structure stability of nanocarriers. The analyses also revealed that although adding crossovers in locations with fewer crossovers increase structural stability, the position of crossovers can have different effects on the stability. DNA origami-based nanocarriers can be implemented in drug delivery, allow the nanocargoes to pass various surfaces and act as filters for passing cargoes of different dimensions and chemical structures.

The results indicate that the number and position of crossovers are among the significant factors in the structure stability of nanocarriers

In this paper, the stability of DNA origami nanocarriers with different positions and numbers of crossovers was investigated.

An efficient protocol for the isolation of high molecular weight DNA from dry powdered samples of turmeric including market samples is described which will help in PCR based detection of adulteration in marketed turmeric powders. The method involves a modified CTAB (3 per cent) procedure with 2 M NaCl, 0.3 per cent β‐mercaptoethanol coupled with purification of DNA in 30 per cent polyethylene glycol (8000). The yield of the DNA obtained from the samples varied from 2 to 4 μg/g tissue. The DNA obtained from the five different samples were consistently amplifiable (RAPD primers).

Briefly reviews previous literature by the author before presenting an original 12 step system integration protocol designed to ensure the success of companies or countries in their efforts to develop and market new products. Looks at the issues from different strategic levels such as corporate, international, military and economic. Presents 31 case studies, including the success of Japan in microchips to the failure of Xerox to sell its invention of the Alto personal computer 3 years before Apple: from the success in DNA and Superconductor research to the success of Sunbeam in inventing and marketing food processors: and from the daring invention and production of atomic energy for survival to the successes of sewing machine inventor Howe in co‐operating on patents to compete in markets. Includes 306 questions and answers in order to qualify concepts introduced.