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A simple and efficient method for separation and purification of troxerutin on the SZ-3 resin

Yuhan Li (College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China)
Xiaolin Mu (College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China)
Haiting Kong (College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China)
Hongchun Pan (College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China)
Hong Liu (College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing, China)

Pigment & Resin Technology

ISSN: 0369-9420

Article publication date: 26 November 2020

Issue publication date: 12 July 2021

100

Abstract

Purpose

In view of the current difficulty of separation of troxerutin, the purpose of the paper is to separate and purify semi-synthetic flavonoid compound troxerutin by macroporous adsorption resin (SZ-3).

Design/methodology/approach

Comparing the adsorption performance and resolution of three different polar resins and choosing a resin to optimize the process parameters such as sample volume, eluent concentration and elution temperature to obtain high-purity troxerutin. After separating and enriching by resin column chromatography, detected the sample by LC-MS analysis.

Findings

This research found that the optimal conditions of the adsorption and desorption were sample volume S = 90 mg/g resin, methanol concentration C = 25%, T = 20 °C. the content of troxerutin increased significantly from 88% to more than 96%. Then confirmed the sample was troxerutin by LC-MS. In addition, the resin could be used for at least 10 cycles in the separation and purification experiments of troxerutin.

Originality/value

Purification of troxerutin with new SZ-3 resin for the first time. Under the optimal conditions, the purity and recovery of troxerutin was 96.4% and 39%. In this study, the authors established a purification process of troxerutin successfully that was simple, economical, environment friendly, with high purity and high recovery rate to provide a reference program for changing the status of troxerutin separation difficulties.

Keywords

Acknowledgements

This work has been supported by Southwest University Dr. Fund projects (SWU110056, SWU110057), Chongqing Engineering Research Center for Pharmaceutical Process and Quality Control Capacity building project (CSTC2012gg-yyjsb10002-33) and Chongqing Postgraduate Education Reform researching project (yjg143085).

Citation

Li, Y., Mu, X., Kong, H., Pan, H. and Liu, H. (2021), "A simple and efficient method for separation and purification of troxerutin on the SZ-3 resin", Pigment & Resin Technology, Vol. 50 No. 4, pp. 338-345. https://doi.org/10.1108/PRT-08-2020-0083

Publisher

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Emerald Publishing Limited

Copyright © 2020, Emerald Publishing Limited

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