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Design and synthesis of thermosensitive target segment resin with precisely controlled distance between immobilization sites for immobilization of penicillin G acylase

Yangdong Liu (State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals, Lanzhou University of Technology, Lanzhou, China)
Siyuan Lu (State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals, Lanzhou University of Technology, Lanzhou, China)
Hongyi Tu (State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals, Lanzhou University of Technology, Lanzhou, China)
Boyuan Zhang (State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals, Lanzhou University of Technology, Lanzhou, China)
Yaqin Zhao (State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals, Lanzhou University of Technology, Lanzhou, China)
Jiasheng He (State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals, Lanzhou University of Technology, Lanzhou, China)
Liangliang He (State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals, Lanzhou University of Technology, Lanzhou, China)
Zhenbin Chen (School of Material Science and Engineering, Lanzhou University of Technology, Lanzhou, China)

Pigment & Resin Technology

ISSN: 0369-9420

Article publication date: 27 January 2023

Issue publication date: 15 July 2024

87

Abstract

Purpose

To save the economic cost and improve the performance of enterprises, this study aims to synthesize high performance immobilized penicillin G acylase (PGA) carriers with fast reaction speed, high recovery rate of enzyme activity and good reusability through corresponding theoretical guidance and experimental exploration.

Design methodology approach

A diblock resin was synthesized by reversible addition-fragmentation chain transfer polymerization method using N, N-diethylacrylamide (DEA) and β-hydroxyethyl methacrylate (HEMA) as functional monomers poly(N, N-diethylacrylamide)-b-poly(β-hydroxyethyl methacrylate) (PDEA-b-PHEMA) was obtained, and the effect of the ratio of DEA and HEMA on the activity of PGA was investigated, and the appropriate block ratio of DEA and HEMA was obtained. After that, the competitive rate of HEMA and glycidyl methacrylate (GMA) under the carrier preparation conditions was investigated. Based on the above work, a thermosensitive resin carrier PDEA-b-PHEMA-b-P(HEMA-co-GMA) with different target distances was synthesized, and the chemical structures and molecular weight of copolymers were investigated by hydrogen NMR (1H NMR).

Findings

The lower critical solution temperature of the resin support decreases with the increase of the monomer HEMA in the random copolymerization; the catalytic performance study indicated that the response rate of the immobilized PGA is fast, and the recovery rate of the enzyme activity of the immobilized PGA varies with the distance between the targets. When the molar ratio of HEMA to GMA in the resin block is 8.15:1 [i.e. resin PDEA100-b-PHEMA10-b-P(HEMA65-co-GMA8)], the activity recovery rate of immobilized PGA can reach 50.51%, which was 15.49% higher than that of pure GMA immobilized PGA.

Originality value

This contribution provides a novel carrier for immobilizing PGA. Under the optimal molar ratio, the enzyme activity recovery could be up to 50.51%, which was 15.49% higher than that of PGA immobilized on the carrier with nonregulated distance between two immobilization sites.

Keywords

Acknowledgements

This work was supported by the National Natural Science Foundation, China (Grant numbers: 51563015); Shenyang National Laboratory for Materials Science and State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals (Grant numbers 18LHPY004 & 18LHZD003).

Yangdong Liu and Siyuan Lu and Hongyi Tu were the first co-author and get the same contribution for this article.

Conflicts of interest or competing interests: The authors declare no competing financial interest.

Citation

Liu, Y., Lu, S., Tu, H., Zhang, B., Zhao, Y., He, J., He, L. and Chen, Z. (2024), "Design and synthesis of thermosensitive target segment resin with precisely controlled distance between immobilization sites for immobilization of penicillin G acylase", Pigment & Resin Technology, Vol. 53 No. 5, pp. 549-556. https://doi.org/10.1108/PRT-06-2022-0076

Publisher

:

Emerald Publishing Limited

Copyright © 2023, Emerald Publishing Limited

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