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Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes

Ljubica Ivanović (Institut Za Javno Zdravlje Crne Gore, Podgorica, Montenegro)
Ana Topalović (Biotechnical Faculty, University of Montenegro, Podgorica, Montenegro)
Višnja Bogdanović (Oncology Institute of Vojvodina, Novi Sad, Serbia)
Dijana Đurović (Health Ecology, Institut Za Javno Zdravlje Crne Gore, Podgorica, Montenegro)
Boban Mugoša (Institut Za Javno Zdravlje Crne Gore, Podgorica, Montenegro)
Milka Jadranin (Institute of Chemistry, Technology and Metallurgy, National Institute, University of Belgrade, Belgrade, Serbia)
Vele Tešević (Faculty of Chemistry, University of Belgrade, Belgrade, Serbia)
Vladimir Beškoski (Faculty of Chemistry, University of Belgrade, Belgrade, Serbia)

British Food Journal

ISSN: 0007-070X

Article publication date: 24 February 2021

Issue publication date: 29 June 2021

184

Abstract

Purpose

The purpose of this paper is to determine the polyphenol content, antioxidative potential and antiproliferative activity of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes.

Design/methodology/approach

Swiss chard was grown in the open field (Lješkopolje, Montenegro) where it was subjected to different fertilization and irrigation regimes. Chard samples were analyzed for previously identified polyphenols and for antioxidant parameters. Additionally, in order to complete the biological activities, chard extracts were tested for antiproliferative activity against MFC-7 and HT-29 tumor cell lines.

Findings

The polyphenols identified in Swiss chard were flavonoids: vitexin-2?-O-xyloside, vitexin-6?-O-acetyl-2?-O-xyloside, vitexin-6?-O-malonyl-2?-O-xyloside and isorhamnetin-3,7-diglucoside. In the antioxidant tests, DPPH (2,2-diphenyl-1-picrylhydrazyl), and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)), chard extract had values of 7.00 and 8.50 (mean values) µmol Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) equ/d.w., respectively. The tested chard extracts inhibited cell proliferation at different concentrations (3.125–50.0 µg/mL) against the MCF-7 cell line, after 24 and 48 h of incubation. The antiproliferative activity, expressed in terms of IC50, was 32.97 and 86.45 µg/mL after 24 h of incubation and 20.76 and 23.33 µg/mL after 48 h of incubation, for treated and untreated chard extracts, respectively.

Originality/value

These data suggest Montenegrin Swiss chard grown under different irrigation and fertilization treatments can be considered as a functional food and should be included in an everyday diet. The collected data could help in the growth improvement of chard with functional food properties.

Keywords

Acknowledgements

This work has been supported by the Ministry of Science of Montenegro and the HERIC project through the BIO-ICT Centre of Excellence (Contract No. 01-1001). This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.Declaration of competing interest: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Citation

Ivanović, L., Topalović, A., Bogdanović, V., Đurović, D., Mugoša, B., Jadranin, M., Tešević, V. and Beškoski, V. (2021), "Antiproliferative activity and antioxidative potential of Swiss chard from Montenegro, grown under different irrigation and fertilization regimes", British Food Journal, Vol. 123 No. 7, pp. 2335-2348. https://doi.org/10.1108/BFJ-11-2020-1062

Publisher

:

Emerald Publishing Limited

Copyright © 2021, Emerald Publishing Limited

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