Determination of heavy metals in cow's and buffalo's fresh raw milk from different areas of Pakistan

Kanza Abid (Institute of Environmental Studies, University of Karachi, Karachi, Pakistan)
Zafar Iqbal Shams (Institute of Environmental Studies, University of Karachi, Karachi, Pakistan)
Muhammad Suleman Tahir (Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, Pakistan)
Arif Zubair (Department of Environmental Science, Federal Urdu University of Arts Sciences and Technology, Karachi, Pakistan)

Arab Gulf Journal of Scientific Research

ISSN: 1985-9899

Article publication date: 17 July 2023

1533

Abstract

Purpose

The presence of heavy metals in milk causes many acute and chronic physiological dysfunctions in human organs. The present study aims to investigate the heavy metals in cow's and buffalo's milk of two major cities, Karachi and Gujranwala, Pakistan to estimate metal intake by humans from this source.

Design/methodology/approach

In total, 48 milk samples from 2 cities were drawn from animals' udder to avoid contamination. Each sample was digested with nitric acid at 105 oC (degree Celsius) on a pre-heated electric hot plate to investigate the metals by atomic absorption spectroscopy (flame type). Air-acetylene technique analyzed chromium, cadmium and lead, and the hydride method analyzed arsenic in the milk samples.

Findings

The results revealed the highest mean lead concentration (19.65 ± 43.86 ppb) in the milk samples, followed by chromium (2.10 ± 2.33 ppb) and arsenic (0.48 ± 0.73 ppb). Cadmium was not detected in any sample, assuming cadmium's occurrence was below the detection level. The concentrations of all the metals in the samples of the two cities do not differ statistically. Lead concentrations in the buffalo's milk were higher than in cow's milk (p < 0.05). However, the concentrations of arsenic and chromium between buffalo's and cow's milk do not differ statistically. The present study reveals a lower level of metals in the milk than those conducted elsewhere. The mean concentrations of all the metals met the World Health Organization's (WHO) safety guidelines (1993).

Research limitations/implications

Although cadmium causes toxicity in the human body, cadmium could not be measured because cadmium's concentration was below the detection level, which is 1 ppb.

Practical implications

This study will help reduce the toxic metals in our environment, and the sources of heavy metals, particularly from the industrial sector could be identified. The feed and water consumed by the milking animals could be carefully used for feeding them.

Social implications

This study will help reduce the diseases and malfunction of human organs and organ systems since these heavy metals cause toxicity and carcinogenicity in humans. Arsenic and chromium cause cancer while lead causes encephalopathy (a brain disease).

Originality/value

The study reports heavy metal concentrations in the two attributes of four independent variables of raw milk samples that were scarcely reported from Pakistan.

Keywords

Citation

Abid, K., Shams, Z.I., Tahir, M.S. and Zubair, A. (2023), "Determination of heavy metals in cow's and buffalo's fresh raw milk from different areas of Pakistan", Arab Gulf Journal of Scientific Research, Vol. ahead-of-print No. ahead-of-print. https://doi.org/10.1108/AGJSR-11-2022-0266

Publisher

:

Emerald Publishing Limited

Copyright © 2023, Kanza Abid, Zafar Iqbal Shams, Muhammad Suleman Tahir and Arif Zubair

License

Published in Arab Gulf Journal of Scientific Research. Published by Emerald Publishing Limited. This article is published under the Creative Commons Attribution (CC BY 4.0) licence. Anyone may reproduce, distribute, translate and create derivative works of this article (for both commercial and non-commercial purposes), subject to full attribution to the original publication and authors. The full terms of this licence may be seen at http://creativecommons.org/licences/by/4.0/legalcode


1. Introduction

The significance of milk for its dietary worth has been widely recognized (Akhtar et al., 2017; Batool et al., 2016; Abdulkhaliq, Swaileh, Hussein, & Matani, 2012) as a valuable source of both micronutrients and macronutrients. Humans essentially require these nutrients for nourishment and regulatory processes Sanz Ceballos et al. (2009). Buffaloes are the main source of milk in Pakistan and are considered as black gold since they fulfill the nutritional requirements of all age groups (Iqbal et al., 2020).

On the contrary, contaminated milk contains some hazardous metals, such as arsenic, cadmium, lead and mercury, which are potentially harmful and cause many physiological dysfunctions in humans (Sager & Hoesch, 2005; Li, McCrory, Powell, Saam, & Jackson-Smith, 2005; WHO, 1996). The presence of these metals has been extensively explored in the animal organs used for milk consumption as these metals emerged in the milk (Burger & Elbin, 2015). Heavy metals may enter the food chain via soil, water and feed, which then find their ways to milk (Zhou, Zheng, Su, Wang, & Soyeurt, 2019). The adverse effects of these contaminants in diets consumed worldwide have threatened human health (Zergui, Boudalia, & Joseph, 2023). These metals are not essential nutrients and not required for any biological function in humans, but are harmful even at low concentrations (Boudebbouz et al., 2020; Varol & Sünbül, 2020). Young children, particularly infants, are at high health risk due to the hazardous metals in the milk because they take it daily for their growth and development. Metal-contaminated milk has harmful consequences on human health even if it has metals in low concentrations (Abu-Darwish, Abu-Dieyeh, Mufeed, Al-Tawaha, & Al-Dalain, 2009; Zheng et al., 2007; Islam, Yang, He, & Mahmood, 2007).

Consequently, many studies demonstrated the occurrence of heavy metals in milk across the world (Boudebbouz et al., 2020; Iftikhar, Arif, Siddiqui, & Khattak, 2014; Abdulkhaliq et al., 2012; Javed et al., 2009; Kazi et al., 2009; Tajkarimi et al., 2008; Caggiano et al., 2005; Licata et al., 2004). US Environmental Protection Agency and Agency for Toxic Substances and Disease Registry listed arsenic, lead and cadmium as the 20 most hazardous and toxic substances (Hameed, Akhtar, Amjada, Naeema, & Tariqa, 2019). The current study was carried out to analyze the concentrations of four hazardous metals, namely arsenic, chromium, lead and cadmium in the milk samples of buffaloes (Bubalus bubalis, L.) and cows (Bos taurus, L.), which were from Karachi and Gujranwala. 12 buffaloes (6 from Karachi and 6 from Gujranwala) and 12 cows ( 6 from Karachi and 6 from Gujranwala) were milked twice daily to collect 24 samples each morning and evening. Further, the effects of the feeding mode on the metal concentrations of the milk were investigated since limited studies are available on this aspect. The study will help reduce the harmful heavy metals in the milk to decrease human diseases and physiological malfunctioning and make it more nourishing, particularly for infants and young children.

2. Material and methods

Two attributes of each of the four independent variables of the milk samples were selected to investigate the statistical difference of the aforesaid toxic metals among them.

  1. 24 samples each from Gujranwala and Karachi were studied to divulge the consistency or the difference in the quantity of various metals in their milk. The two cities are 1200 km away from each other (Figure 1). Karachi is the most populous city and economic hub of the country, located in Sindh Province along the Arabian Sea. Gujranwala is the 7th most populous city, located in the Punjab Province of Pakistan. Both cities have different climates and geography.

  2. 24 samples each in the Morning and Evening were taken to show the temporal consistency or the difference in the amount of different metals.

  3. 24 samples each of cows and buffalos were taken to reveal the consistency or the difference in the quantity of different metals (Ahmad et al., 2008; Fundora et al., 2001)

  4. 24 samples of each of the control and uncontrol feeding modes of the animals to demonstrate the consistency or the difference in the quantity of different metals. The animals of dairy farms were ‘Controlled Feeding' while the roaming buffaloes and cows that feed on debris and municipal solid waste were ‘Uncontrol Feeding'.

A total of 48 milk samples were analyzed to determine whether the toxic metals differ or not significantly between each of the above variables of the samples. In addition, the milk samples from animals were extracted twice daily (morning & evening) to determine the temporal difference in their metal concentrations (Supplementary data).

2.1 Sample collection

The milk samples of both animal species were directly drawn from their udder to avoid contamination during transportation and retailing because retail shops generally vend adulterated milk. The milk is usually mixed and diluted with water in the retail shop or during its supply chain (Arif et al., 2020).

The milk of both the animal species of the controlled environment was extracted twice daily after serving them fodder in the morning and evening hours. However, the milk of the animals in the uncontrolled environment is extracted in the morning without serving them fodder. After taking out milk, they are set free for food from municipal debris and other wastes, which commonly exist under bridges and along the city roadsides. However, the animals' milk in the uncontrolled environment is extracted in the evening after serving them as fodder on the dairy farms. In the present study, 48 samples were from twelve dairy farms, six samples each from Karachi and Gujranwala, located in different areas. Each milk sample has three replicates.

2.2 Sample digestion

The milk samples were taken in transparent plastic bottles, which were instantly labeled and then placed into the freezer. Approximately 50 ml of milk was taken as a sample from each animal for the study.

Each milk sample was digested by a low-priced digestion method (AOAC, 2000). This method was selected to keep the digestion process less contaminated and simple. Analytical-reagent-grade of 65% concentrated nitric acid in its highest purity (E. Merck, Darmstadt, Germany) was used in this experiment. 10 ml of the sample was added to 5 ml of concentrated HNO3 and then heated at 105 °C on a pre-heated electric hot plate until the solution become transparent. The sample solution was then filtered with Whatman filter paper # 40. The filtered digested sample was made 100 ml by adding deionized water.

2.3 Toxicological analysis

The filtered samples were investigated by PG instrument (UK) AA500 atomic absorption spectroscopy (flame type) in the laboratory of Space and Upper Atmosphere Research Commission (SUPARCO). The instrument, equipped with flame atomizer, air-acetylene (standard configuration), computer and AA Win software, analyzed chromium, cadmium and lead. Calibration was done by 3 points, with one duplicate sample and standards used for calibration were bought from Perkin Elmer, USA. Hydride method analyzed arsenic in the milk samples. The lead was kept at 217.0 nm wavelength and cadmium, chromium and arsenic at 228.8 nm, 375.9 nm and 193.7 nm wavelength, respectively. The slit was kept at 0.5 nm during the analysis of all four metals. It is worth mentioning that the concentration of the standard solution of 0.1 ppb, 1.0 ppb and 10 ppb was for arsenic, while for the other three metals, the concentrations were 0.1 ppm, 1.0 ppm and 10 ppm.

LOD was calculated from following equation:

LOD=LOB+1.645(SDoflowconcentrationsample)
Whereas,
LOB=meanofblank+1.645(SDofblank)

2.4 Statistical analysis

Kruskal–Wallis H test was applied to determine the statistical difference among concentrations of three metals in the milk samples and each metal among four variables. Mann–Whitney U test was performed to determine the statistical difference between two attributes of each of the four variables (Table 2).

3. Results

Four metals, namely arsenic, cadmium, chromium and lead were investigated in the forty-eight milk samples. The present study demonstrated zero cadmium in all the milk samples. Its concentration is assumed to be below the detection level, so excluded from further analysis. The detection level of the Atomic Absorption Spectroscopy was one ppb. Karachi's samples contained the highest mean chromium concentration (2.40 ± 2.61 ppb). Gujranwala's samples divulged the highest mean arsenic concentration (0.59 ± 0.88 ppb). The buffaloes' milk showed the highest mean lead concentration (28.43 ± 59.98 ppb) (Table 1). The lead levels were significantly higher (p < 0.01) than the levels of arsenic and chromium.

The arsenic concentrations in the milk samples of the two cities do not differ significantly. Similarly, non-significant differences were divulged between two attributes of arsenic levels for the other three independent variables, that is, between the milk samples of buffaloes and cows, the milk samples of controlled and uncontrolled feeds and between the arsenic levels of the milk samples of morning and evening. The chromium demonstrated the same results. However, the lead concentrations in the cow's and buffalo's milk samples differ significantly (p = 0.02), while the two attributes of lead concentrations did not differ significantly in the other three independent variables (Table 2).

3.1 Box-and-whisker plot

The concentrations of different metals in the milk of different categories are plotted in the box-and-whisker diagram, which is non-parametric visual descriptive statistics. Figure 2 illustrates the box plots of chromium levels in the milk samples of eight groups. The box reveals an outlier of chromium concentration in the milk of Gujranwala while two outliers each of chromium concentrations are found in the milk of cows and both the animal species that took uncontrolled feed.

Figure 3 demonstrates the box plot of arsenic levels in the samples of two different attributes and four variables. All the attributes and variables show outlier values of arsenic in the samples. The animals of Gujranwala and the animals that took uncontrolled feed reveal one outlier value for each arsenic in their milk samples. Other categories exhibit a higher number of outliers of arsenic in the samples.

Figure 4 presents the box plot of lead levels in the milk samples of various attributes and variables. The box plot shows one outlier concentration each of lead in the milk samples of Karachi and Gujranwala whereas two outlier values each are found in the milk of buffaloes, in the milk of the animals that took the uncontrolled feed and in the milk samples that were extracted in the morning session.

In addition, all three box-and-whisker plots reveal the means, quartiles and standard deviations of the concentrations of all the attributes and variables.

4. Discussion

The results divulge that all three metals do not significantly differ between their two different attributes for three variables, namely Cities, Time of Milking and Mode of Feed. The arsenic levels in the milk samples of Gujranwala and Karachi did not differ significantly. Similarly, the lead concentrations in the milk samples of the two cities did not differ significantly. The chromium levels in the milk samples from the two conurbations also did not differ statistically.

Similarly, all the heavy metals concentrations in the milk samples demonstrated no statistical differences between controlled and uncontrolled feeds and between the milk samples collected in the morning and evening. However, the lead concentrations differ significantly between the milk samples of cow and buffalo (Table 2). It is worth mentioning that cadmium remained undetected in milk samples of both cities. It may exist below the detectable level in the samples. In the current study, the minimum detection level was one ppb, an extremely low concentration. It concluded that the milk is not tainted with cadmium.

However, the contamination of cadmium in the milk samples is widely detected. For instance, Hossain, Tarafder, Hasan, Kabir, and Azad (2017) reported that the milk of roaming animals in Dhaka, Bangladesh contains 7.52 ppm cadmium on average. The animals feed on municipal garbage that has lower cadmium concentrations. The leaves and the dust in the urban environment are contaminated with lead that might have entered into animals' fodder (Farid, Shams, & Khan, 2017; Shams & Beg, 2000). Moreover, cadmium was found higher in their feces. This could be due to the accumulation of metals in their body. Kazi et al. (2009) found cadmium in both the processed and raw milk of Hyderabad, Pakistan. Iftikhar et al. (2014) detected greater cadmium concentration in the animals' milk of the rural expanses of Peshawar and compared their findings with results, which were in the reports of the Joint Expert Committee of Food and Agriculture Organization (FAO)/World Health Organization (WHO) (1989). Arif et al. (2020), reported 0.021 ± 0.006 ppm as the mean cadmium concentration in raw milk samples of animals collected from different urban districts of Lahore. Whey, watery, or liquid fraction of milk generally contains cadmium. It does not attach to fat and caseins. Unlike other toxic metals, cadmium attaches to objects of smaller molecular weight, like citrates, lactose and whey proteins (Kazi et al., 2009). Su et al. (2021) revealed a higher concentration of chromium and arsenic in the milk of the cows that were reared and fed in the industrial areas of China compared to those in the non-industrial areas.

The present study demonstrates considerable variations in the lead levels in the milk. The lead levels in 48 milk samples ranged from 0.0 to 294.37 ppb. The median lead levels in the milk samples of the eight groups were between 6.19 to 11.66 ppb. The median lead level of cows' milk was 6.19 ppb, while that of buffaloes was 11.66 ppb. The median lead levels of the other six categories were within these values. Likewise, mean lead levels in the samples of eight different groups were between 9.67 ± 11.65 ppb to 28.43 ± 60.92 ppb. A significantly higher lead concentration was found in the buffalo’s milk compared to that of cow’s milk (Table 2). Khan et al. (2014) also found higher lead levels in buffaloes' milk than the cows' milk. These studies suggest that the milk of cows is less contaminated with lead than that of buffaloes. However, no significant difference was noted between the attributes of different variables.

The environmental lead goes into the food chain through the water and fodder on which the animals are raised. Environmental lead is an important factor in escalating the lead level in animals' milk (Kazi et al., 2009; Sikirić, Brajenović, Pavlović, Havranek, & Plavljanić, 2003). Its accumulation rate depends on the size and age of the animal. According to Croatian Legislation, 100 ppb is the tolerance limit of lead (Narodne Novine, 1994). Of 48 milk samples, the lead level in one milk sample exceeded 100 ppb. The lead of other milk samples was within 100 ppb. The joint FAO/WHO Committee in 1993 on Food Additives and Contaminants recommended 20 ppb lead as the maximum permissible limit for raw milk. Khan, Ullah, Wahid, Sultana, and Rahim (2021) reported a mean level of 1.12 ppm in raw milk samples in Peshawar, which exceeds the WHO maximum permissible lead limit for raw milk. Arif et al. (2020) reported 0.024 + 0.005 ppm lead in the raw milk samples collected from urban areas of Lahore city.

However, Javed et al. (2009) found a higher lead level (18.87 ppm) in the animals' milk from Faisalabad. They suggested that the industries in the city are responsible for environmental lead, which contaminates the animals' milk. Khan et al. (2014) explored the lead levels in the cattle's milk from Swat and found that the fodder and water contaminated the cattle's milk. Iftikhar et al. (2014) noted 2.24 ppm lead residues in the animals' milk of Peshawar City, which was higher than that of the current study. They have not found any difference in the lead levels of milk in suburban and urban areas of the city. Arif et al. (2020) reported that the concentrations of lead and cadmium exceeded the maximum permissible limit recommended by the International Dairy Federation. However, Shar, Pirhot, Shar, and Channa (2021) reported that the mean lead concentrations in the milk samples of cows and buffalos met the safety guidelines. They found 3.1 ppb and 2.8 ppb lead in the milk samples of cows and buffalos of Hyderabad, Pakistan.

The arsenic levels in the milk samples of the present study were significantly lower compared to those of lead and chromium. The arsenic concentrations in 48 milk samples ranged from 0.0 to 3.70 ppb. The median arsenic concentrations in the samples of eight different categories were calculated between 0.17 to 0.30 ppb. The median arsenic concentration of cows' milk was 0.17 ppb, while that of buffaloes was 0.30 ppb. The median arsenic levels of the other six categories were within these concentrations. Similarly, mean arsenic concentrations in the milk samples of eight different groups ranged between 0.40 ± 0.55 ppb to 0.59 ± 0.88 ppb. Unlike lead, Gujranwala shows higher arsenic concentrations (0.59 + 0.88 ppb) in their milk samples than the arsenic level (0.40 ± 0.55 ppb) in the milk from Karachi. This may be due to the arsenic in the groundwater or soil of some areas of the Punjab Province. However, no significant difference was noted between the attributes of three variables.

Chromium concentration in the milk samples was lower than lead concentration and higher than the arsenic levels. Chromium concentrations in 48 milk samples ranged from 0.0 to 8.60 ppb. The median chromium in the samples of eight groups ranged between 0.0 to 1.60 ppb. The mean chromium levels in the milk of eight different categories were between 1.77 ± 2.05 ppb to 2.40 ± 2.61 ppb. Unlike arsenic, Karachi reveals higher chromium concentrations (2.40 ± 2.61 ppb) in their milk samples than the chromium level (2.40 ± 2.61 ppb) in the milk from Gujranwala. Both the values meet the WHO guidelines of maximum residual concentration (25 ppb) for chromium. Karachi is an industrial city that accommodates many chromium-releasing industries, particularly chrome tanning industries, which could be a possible source of chromium contamination. Nonetheless, the attributes of different variables do not differ significantly. Javed et al. (2009) found greater mean concentrations (1.07 ± 0.07 ppm) of chromium in the animals’ milk of Faisalabad City.

It is observed that the fodders of the animals are also irrigated with industrial wastewater that sometimes contains a very high level of toxic metal. This suggests that the fodders of controlled feed are contaminated with toxic, which may go into the food chain and find their way into the milk of the animals. In the uncontrolled feed category, both animal species take their food from other sources. Alam, Kabir, Sakib, Salahuddin, and Azad (2016) and Hossain et al. (2017) investigated the toxic metals in the milk of animals nourished on municipal solid waste. Hossain et al. (2017) found very high chromium concentrations (10.99 ppm) in the milk of roaming animals in Dhaka, Bangladesh. These animals take uncontrolled feed. However, very few studies exist to establish any relationship between the different toxic metals in the milk due to diverse feeding modes.

In the current study, all the metals in the buffaloes' milk were higher than those of cows. It may be because of the tendency of the metals to accumulate in the lipid content of the milk since buffalo milk contains higher lipids than that of cows. Jan et al. (2011) reported that buffalo milk has a higher tendency toward harmful metal accumulation than the milk of other animals. This may be due to the lipophilic properties of heavy metals, which form lipophilic complexes in the milk. Khan et al. (2013) and Khan, Malik, Muhammad, Ullah, and Qadir (2015) reported an upsurge of the toxic metals in the animals’ milk of Swat, Pakistan. They recorded 68 ppb of lead, 455 ppb of chromium and 98 ppb of cadmium in the milk, fruits and grains, which were higher than their earlier report.

Worldwide, the milk of cows and buffaloes are vital source of nutrients for nourishing human health, particularly the growth and development of infants and young children (Tripathi, Raghunath, Sastry, & Krishnamoorthy, 1999). However, the dangerous metals in the milk samples lower their health benefits and elevate their toxicity. The infants and the children are the high-risk sections of the population since they generally take milk daily as their regular feed. The intake of contaminated milk may cause health effects, particularly in growing children, namely dysfunctions of the brain, kidneys and liver. For instance, young ones develop encephalopathy when exposed to lead. The increase in the heavy metal concentration in human blood may cause disturbances in cardiovascular systems (Salma, Maenhaut, Dubtsov, Zemplen-Papp, & Zaray, 2000). For that reason, it suggests minimizing and controlling the load of toxic metals in dairy milk at the national level.

5. Conclusion

The present study suggests that fresh raw milk of Karachi and Gujranwala contains lower concentrations of lead, chromium and arsenic than the studies conducted elsewhere, which may be because the milk samples were extracted directly from animals' udder. In other studies, the milk samples were from retail shops or tetra packs, which could have been contaminated during handling and supplies from dairy farms to retail markets. Moreover, it could have been contaminated through dilution with tap water, which is usually added to expand the volume of the milk for shady earnings (Arif et al., 2020). The addition of contaminated water in the milk for increasing its volume is a primary aspect of metal contamination in the milk. A non-significant difference was found between the two attributes of three independent variables. The metal concentrations of the milk from Karachi do not differ significantly from those from Gujranwala. Likewise, the metal concentrations of the milk extracted during the morning were similar to those extracted in the evening. In the same way, no significant difference was found between the milk of controlled-fed and uncontrolled-fed animals. However, lead concentrations in buffalo milk were significantly (p = 0.02) higher from those in cow milk.

6. Policy recommendation

The present study demonstrates that the concentrations of lead, chromium, cadmium and arsenic met the maximum permissible limit recommended by the WHO. However, it suggests that the milk during the supply chain, i.e. from farmland to retail shops, should be checked for heavy metal contamination. This study could be used as a reference for heavy metal contamination during handling and supplies.

Figures

Location of Karachi and Gujranwala in Pakistan

Figure 1

Location of Karachi and Gujranwala in Pakistan

Box-and-whisker plot of chromium concentration in the milk of different categories

Figure 2

Box-and-whisker plot of chromium concentration in the milk of different categories

Box-and-whisker plot of arsenic concentration in the milk of different categories

Figure 3

Box-and-whisker plot of arsenic concentration in the milk of different categories

Box-and-whisker plot of lead concentration in the milk of different categories

Figure 4

Box-and-whisker plot of lead concentration in the milk of different categories

Descriptive statistics of toxic metal levels (in ppb) in the milk of buffaloes and cows

PollutantsAttributesCitiesAnimal speciesFeeding modeMilking time
KarachiGujranwalaCowBuffaloControlled feedUncontrolled feedMorningEvening
ArsenicQ10.15000.170.15000.14
Median0.250.220.170.30.270.180.220.26
Q30.400.690.530.630.450.630.530.45
Mean0.400.590.480.570.480.510.410.58
± SD±0.55±0.88±0.84±0.65±0.79±0.69±0.51±0.90
LeadQ16.19007.565.6603.531.21
Median11.047.536.1911.669.3310.29.8710.01
Q321.2517.9116.3722.8721.3117.9127.4712.94
Mean16.6421.469.6728.4313.2424.8628.0310.07
± SD±20.64±58.98±11.65±59.98±11.64±60.92±60.54±9.11
ChromiumQ10000.8301.200
Median1.501.201.151.6001.601.201.40
Q33.302.482.333.233.452.552.353.23
Mean2.401.771.862.311.972.201.782.39
± SD±2.61±2.05±2.44±2.28±2.79±1.85±1.87±2.75

Source(s): Table by authors

P-values demonstrating statistical difference between attributes and variables

AttributesPollutants
LeadArsenicChromium
VariablesKarachiGujranwala0.26*1.00*0.45*
CowBuffalo0.03**0.28*0.24*
MorningEvening0.43*0.79*0.57*
ControlledUncontrolled0.58*0.29*0.09*

Note(s): *Non-significant, **Significant

Source(s): Table by authors

S.#CityAnimalMilking timeType of feedConcentration of metals (ppb)
Lead (Pb)Arsenic (As)Chromium (Cr)
43GujranwalaBuffaloEveningControlled11.560.420
44GujranwalaBuffaloEveningControlled1.210.132.9
45GujranwalaBuffaloEveningControlled9.950.265.3
31GujranwalaCowEveningControlled6.343.72.4
32GujranwalaCowEveningControlled000
33GujranwalaCowEveningControlled21.650.620
37GujranwalaBuffaloMorningControlled11.030.411.2
38GujranwalaBuffaloMorningControlled8.710.151.6
39GujranwalaBuffaloMorningControlled6.1100
25GujranwalaCowMorningControlled000
26GujranwalaCowMorningControlled4.291.80
27GujranwalaCowMorningControlled28.280.180
46GujranwalaBuffaloEveningUncontrolled27.320.360
47GujranwalaBuffaloEveningUncontrolled12.330.161.3
48GujranwalaBuffaloEveningUncontrolled21.311.84.2
34GujranwalaCowEveningUncontrolled008.2
35GujranwalaCowEveningUncontrolled002.7
36GujranwalaCowEveningUncontrolled2.631.61.2
40GujranwalaBuffaloMorningUncontrolled294.370.911.2
41GujranwalaBuffaloMorningUncontrolled16.770.534.5
42GujranwalaBuffaloMorningUncontrolled31.091.11.9
28GujranwalaCowMorningUncontrolled001.08
29GujranwalaCowMorningUncontrolled001.7
30GujranwalaCowMorningUncontrolled001.1
19KarachiBuffaloEveningControlled31.100.338.6
20KarachiBuffaloEveningControlled000
21KarachiBuffaloEveningControlled7.660.175.3
7KarachiCowEveningControlled7.120.367.7
8KarachiCowEveningControlled21.200.530
9KarachiCowEveningControlled14.760.160
13KarachiBuffaloMorningControlled1.2200
14KarachiBuffaloMorningControlled7.240.251.1
15KarachiBuffaloMorningControlled27.290.276.1
1KarachiCowMorningControlled41.860.90
2KarachiCowMorningControlled21.200.530
3KarachiCowMorningControlled28.010.275.1
22KarachiBuffaloEveningUncontrolled1.200.251.6
23KarachiBuffaloEveningUncontrolled11.752.41.8
24KarachiBuffaloEveningUncontrolled10.320.390
10KarachiCowEveningUncontrolled001.3
11KarachiCowEveningUncontrolled10.070.171.4
12KarachiCowEveningUncontrolled12.220.141.4
16KarachiBuffaloMorningUncontrolled12.780.111.6
17KarachiBuffaloMorningUncontrolled21.401.62.5
18KarachiBuffaloMorningUncontrolled98.590.182.7
4KarachiCowMorningUncontrolled005.9
5KarachiCowMorningUncontrolled6.190.412.3
6KarachiCowMorningUncontrolled6.190.171.1

Source(s): Table by authors

Compliance with ethical standards:

Ethical approval: This article does not contain any studies with human participants or animals performed by the authors.

Conflict of interest: The authors declare that they have no conflict of interest.

Supplementary data

Table S1

References:

Abdulkhaliq, A., Swaileh, K. M., Hussein, R. M., & Matani, M. (2012). Levels of metals (Cd, Pb, Cu, and Fe) in cow's milk, dairy products, and hen's eggs from the West Bank, Palestine. International Food Research Journal, 19(3), 10891094.

Abu-Darwish, M. S., Abu-Dieyeh, Z. H., Mufeed, B., Al-Tawaha, A. R. M., & Al-Dalain, S. Y. A. (2009). Trace element contents and essential oil yields from wild thyme plants (Thymus serpyllum L.) grown in different natural variable environments, Jordan. Journal Food Agriculture & Environment, 7(3 & 4), 920924.

Ahmad, S., Gaucher, I., Rousseau, F., Beaucher, E., Piot, M., Groungnet, J. F., & Gaucheron, F. (2008). Effects of acidification on physicochemical characteristics of buffalo milk: A comparison with cow's milk. Food Chemistry, 106(1), 1117.

Akhtar, S., Shahzad, M. A., Yoo, S.-H., Ismail, A., Hameed, A., Ismail, T., & Riaz, M. (2017). Determination of aflatoxin M1 and heavy metals in infant formula milk brands available in Pakistani markets. Korean Journal for Food Science of Animal Resources, 37(1), 79.

Alam, M. N., Kabir, A. K. M. A., Sakib, M. N., Salahuddin, M., & Azad, M. A. K. (2016). Impact of livestock rearing practices on public health and environmental issues in selected municipality areas of Bangladesh. Bangladesh Journal of Animal Science, 45(1), 4451.

AOAC (Association of Official Analytical Chemists) (2000). Official methods of analysis international (17th Ed). Washington, DC: AOAC.

Arif, A.M., Javed, I., Ayaz, M., Abdullah, M., Imran, M., Rashid, A., … Martorell, M. (2020). Chemical composition, adulteration, total microbial load, and heavy metal in raw milk samples collected from dairy farms and urban areas in Lahore District, Pakistan. Journal of Food Safety, 40(1), e12729.

Batool, F., Iqbal, S., Tariq, M. I., Akbar, J., Noreen, S., Danish, M., & Chan, K. W. (2016). Milk: Carrier of heavy metals from crops through the ruminant body to human beings. Journal of the Chemical Society Pakistan, 38(01), 39.

Boudebbouz, A., Boudalia, S., Bousbia, A., Habila, S., Boussadia, M. I., & Gueroui, Y. (2020). Heavy metals levels in raw cow milk and health risk assessment across the globe: A systematic review. Science of the Total Environment, 141830.

Burger, J., & Elbin, S. (2015). Metal levels in eggs of waterbirds in the New York Harbor (USA): Trophic relationships and possible risk to human consumers. Journal of Toxicology and Environmental Health, A, 78(2), 7891.

Caggiano, R., Sabia, S., D'Emilio, M., Macchiato, M., Anastasio, A., Ragosta, M., & Paino, S. (2005). Metal levels in fodder, milk, dairy products, and tissues were sampled in ovine farms in Southern Italy. Environmental Research, 99, 4857.

Farid, H., Shams, Z. I., & Khan, F. A. (2017). Lead concentration in urban dust and in leaves of street plants, Karachi. Kuwait Journal of Science, 44(2), 129135.

Fundora, O., Gonzalez, M. E., Lezcano, O., Montejo, A., Pompa, N., & Enriquez, A. V. (2001). A comparative study of milk composition and stability of Murrah river buffaloes and Hoistein cows grazing star grass. Cuban Journal of Agricultural Science, 35, 219222.

Hameed, A., Akhtar, S., Amjada, A., Naeema, I., & Tariqa, M. (2019). Comparative assessment of arsenic contamination in raw milk, infant formulas and breast milk. Journal of Dairy & Veterinary Sciences, 13(1), 555851.

Hossain, A. S. S., Tarafder, M. M. A., Hasan, M. N., Kabir, A. K. M. A., & Azad, M. A. K. (2017). Garbage waste induced heavy metals on roaming cattle. Bangladesh Journal of Animal Science, 46(1), 2428.

Iftikhar, B., Arif, S., Siddiqui, S., & Khattak, R. (2014). Assessment of toxic metals in dairy milk and animal feed in Peshawar, Pakistan. British Biotechnology Journal, 4(8), 883893.

Iqbal, Z., Abbas, F., Ibrahim, M., Qureshi, T.I., Gul, M., & Mahmood, A. (2020). Human health risk assessment of heavy metals in raw milk of buffalo feeding at wastewater-irrigated agricultural farms in Pakistan. Environmental Science and Pollution Research, 27, 2956729579.

Islam, E., Yang, X. E., He, Z. L., & Mahmood, Q. (2007). Assessing potential dietary toxicity of heavy metals in selected vegetables and food crops. J Zhejiang Univ Sci B, 8(1), 113.

Jan, F. A., Ishaq, M., Khan, S., Shakirullah, M., Asim, S. M., Ahmad, I., & Mabood, F. (2011). Bioaccumulation of metals in human blood in industrially contaminated areas. Journal of Environmental Sciences, 23(12), 20692077.

Javed, I., Jan, I., Muhammad, F., Zia-ur-Rahman, Khan, M. Z., Aslam, B., & Sultan, J. I. (2009). Heavy metal residues in the milk of cattle and goats during winter season. Bulletin of Environmental Contamination and Toxicology, 82(5), 616620.

Joint FAO/WHO Expert Committee on Food Additives FAO & WHO. (1989), Evaluation of Certain Food Additives and Contaminants: Thirty-Third Report of the Joint FAO/WHO Expert Committee on Food Additives [meeting held in Geneva from 21 to 30 March 1988], World Health Organization, available at: https://apps.who.int/iris/handle/10665/39252

Kazi, T. G., Jalbani, N., Baig, J. A., Kandhro, G. A., Afridi, H. I., Arain, M. B., … Shah, A. Q. (2009). Assessment of toxic metals in raw and processed milk samples using electrothermal atomic absorption spectrophotometer. Food and Chemical Toxicology, 47(9), 21632169.

Khan, K., Lu, Y., Khan, H., Ishtiaq, M., Khan, S., Waqas, M., … Wang, T. (2013). Heavy metals in agricultural soils and crops and their health risks in Swat District, northern Pakistan. Food and Chemical Toxicology, 58, 449458.

Khan, K., Khan, H., Lu, Y., Ihsanullah, I., Nawab, J., Khan, S., … Maryam, A. (2014). Evaluation of toxicological risk of foodstuffs contaminated with heavy metals in Swat, Pakistan. Ecotoxicology and Environmental Safety, 108, 224232.

Khan, M. U., Malik, R. N., Muhammad, S., Ullah, F., & Qadir, A. (2015). Health risk assessment of consumption of heavy metals in market food crops from Sialkot and Gujranwala Districts, Pakistan. Human and Ecological Risk Assessment, 21(2), 327337.

Khan, N., Ullah, R., Wahid, F., Sultana, Q., & Rahim, M. (2021). Spectrophotometric quantification of trace elements and toxic metals in raw milk samples of peshawar city, Pakistan. International Journal of Economic and Environmental Geology, 12(1), 5359.

Li, Y., McCrory, D. F., Powell, J. M., Saam, H., & Jackson-Smith, D. (2005). A survey of selected heavy metal concentrations in Wisconsin Dairy Feeds. Journal Dairy Science, 88(8), 29112922.

Licata, P., Trombetta, D., Cristani, M., Giofre, F., Martino, D., Calo, M., & Naccari, F. (2004). Levels of ‘‘toxic’’ and ‘‘essential’’ metals in samples of bovine milk from various dairy farms in Calabria, Italy. Environment International, 30(1), 16.

Narodne Novine (1994). Regulation of allowed pesticides, toxins, mycotoxins, metals and histamine and similar substances in food. Narodne Novine, 46, 1583.

Sager, M., & Hoesch, J. (2005). Macro and micro-element levels in cereals grown in lower Austria. Journal of Central European Agriculture, 6(4), 461467.

Salma, I., Maenhaut, W., Dubtsov, S., Zemplen-Papp, E., & Zaray, G. (2000). Impact of phase-out of leaded gasoline on the air quality in Budapest. Microchemical Journal, 67, 127133.

Sanz Ceballos, L., Morales, E. R., de la Torre Adarve, G., Diaz Castro, J., Martínez, L. P., & Sanz Sampelayo, M. R. (2009). Composition of goat and cow milk produced under similar conditions and analyzed by identical methodology. Journal of Food Composition and Analysis, 22(4), 322329.

Shams, Z. I., & Beg, M. A. A. (2000). Lead in particulate deposits and in leaves of roadside plants, Karachi, Pakistan. The Environmentalist, 20, 6367.

Shar, Z.H., Pirhot, O.P., Shar, H.H., & Channa, M.K. (2021). Assessing the risk of heavy metals contamination in milk from Pakistan. Int. J. Sci. Res. Arch, 3(01), 107113.

Sikirić, M., Brajenović, N., Pavlović, I., Havranek, J. L., & Plavljanić (2003). Determination of metals in cow’s milk by flame atomic absorption spectrophotometry. Czech Journal of Animal Science, 48(11), 481486.

Su, C., Gao, Y., Qu, X., Zhou, X., Yang, X., Huang, S., & Wang, J. (2021). The occurrence, pathways, and risk assessment of heavy metals in raw milk from industrial areas in China. Toxics, 9(12), 320.

Tajkarimi, M., Faghih, M. A., Poursoltani, H., Nejad, A. S., Motallebi, A. A., & Mahdavi, H. (2008). Lead residue levels in raw milk from different regions of Iran. Food Control, 19(5), 495498.

Tripathi, R. M., Raghunath, R., Sastry, V. N., & Krishnamoorthy, T. M. (1999). Daily intake of heavy metals by infants through milk and milk products. Science of The Total Environment, 227(2-3), 229235.

Varol, M., & Sünbül, M. R. (2020). Macroelements and toxic trace elements in muscle and liver of fish species from the largest three reservoirs in Turkey and human risk assessment based on the worst-case scenarios. Environmental Research, 184, 109298.

World Health Organization, (WHO) (1996). Geneva, Switzerland. Trace elements in human nutrition and health.

Zergui, A., Boudalia, S., & Joseph, M.L. (2023). Heavy metals in honey and poultry eggs as indicators of environmental pollution and potential risks to human health. Journal of Food Composition and Analysis, 119, 105255.

Zheng, N., Wang, Q., Zhang, X., Zheng, D., Zhang, Z., & Zhang, S. (2007). Population health risk due to dietary intake of heavy metals in the industrial area of Huludao city, China. Science of The Total Environment, 387(1-3), 9610.

Zhou, X., Zheng, N., Su, C., Wang, J., & Soyeurt, H. (2019). Relationships between Pb, As, Cr, and Cd in individual cows’ milk and milk composition and heavy metal contents in water, silage, and soil. Environmental Pollution, 255, 113322.

Further reading

World Health Organization (WHO) (1993). Evaluation of certain food additives and contaminants. WHO Technical Report Series Number 837.

Corresponding author

Zafar Iqbal Shams can be contacted at: zishams@uok.edu.pk

Related articles