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The purpose of this paper is to evaluate subjectivity issue, particularly sensitivity variance and fatigue effect, in human sensory evaluation, as well as review the feasibility of human-independent quality system, using E-tongue and E-nose.

The sensitivity level is evaluated by measuring the threshold of Acesulfame-K, while the fatigue effect is evaluated by measuring the accuracy level of evaluation through the time. The experiment was administered to six trained sensory panelists.

The experiment result shows that each panelist has a different level of sensitivity and tendency in evaluating samples containing Acesulfame-K. Furthermore, by simulating the panelists’ daily inspection, the fatigue effect is also found in one out of six panelists. The use of E-nose and E-tongue, may eliminate the subjectivity issue, supporting the development of human error-free quality system.

The research findings indicate the needs of human substitution-built into the quality system to avoid both of subjectivity and error judgment while defining the products quality. However, the small numbers of panelists as well as the unvalidated substitute instruments application in the target workcenter were the main limitation of this study. Human-independent quality system could be applied only when the instruments have been calibrated to human response in perceiving taste and odor.

The research finding supports the theory of human panels’ tradeoffs in a sensory analysis in terms of sensitivity level variance and fatigue. It has provided additional contributions to the existing theories as well as developed effective strategies for the development of the human-independent quality system.