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Developing an electrochemical immunosensor for early diagnosis of hepatocellular carcinoma

Abdulrahman Al-Shami (Institute of Nanotechnology, Jordan University of Science and Technology, Irbid, Jordan)
Rami Joseph Oweis (Institute of Nanotechnology, Jordan University of Science and Technology, Irbid, Jordan)
Mohamed Ghazi Al-Fandi (Institute of Nanotechnology, Jordan University of Science and Technology, Irbid, Jordan)

Sensor Review

ISSN: 0260-2288

Article publication date: 8 April 2021

Issue publication date: 17 May 2021

132

Abstract

Purpose

This paper aims to report on the development of a novel electrochemical amperometric immunosensor to diagnose early hepatocellular carcinoma (HCC) by detecting the Midkine (MDK) biomarker.

Design/methodology/approach

Anti-Midkine antibodies were immobilized covalently through carbodiimides chemistry on carbon screen-printed electrodes modified with carboxylated multi-walled carbon nanotubes. The development process was characterized using cyclic voltammetry, electrochemical impedimetric spectroscopy, Fourier transform infrared spectroscopy and atomic force microscopy. Differential pulse voltammetry was used to investigate the immunosensor performance in detecting MDK antigen within the concentration range of 1 pg/ml to 100 ng/ml.

Findings

MDK immunosensor exhibited high sensitivity and linearity with a detection limit of 0.8 pg/ml and a correlation coefficient of 0.99. The biosensor also demonstrated high selectivity, stability and reproducibility.

Originality/value

The developed MDK immunosensor could be a promising tool to diagnose HCC and reduce the number of related deaths.

Keywords

Acknowledgements

This work was funded by the deanship of research at Jordan University of Science and Technology through grant No. 20180428.

Citation

Al-Shami, A., Oweis, R.J. and Al-Fandi, M.G. (2021), "Developing an electrochemical immunosensor for early diagnosis of hepatocellular carcinoma", Sensor Review, Vol. 41 No. 2, pp. 125-134. https://doi.org/10.1108/SR-02-2020-0030

Publisher

:

Emerald Publishing Limited

Copyright © 2021, Emerald Publishing Limited

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