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The purpose of this paper is to evaluate the possibility of a new biocatalyst prepared by kefir cells immobilization on grape stalks (GS) to reduce quickly and efficiently the Biochemical Oxygen Demand (BOD) and Chemical Oxygen Demand (COD) of the waste whey during its fermentation producing potable ethanol.

Many batch whey fermentations were carried out in order the effect of various conditions (pH, temperature) on 14C-labeled lactose uptake rate by the GS-immobilized kefir cells and consequently on fermentation rate as well as on ethanol production and whey BOD and COD reduction to be determined.

It has been illustrated that GS-biocatalyst was suitable for whey BOD and COD reduction by about 32 and 25 percent, respectively during whey alcoholic fermentation at 30°C and pH 5.5 in only ten hours, producing about 3.30 percent w/v of ethanol.

The findings of this research may enhance the existing literature on whey exploitation, for the first time focussing on the use of cheap and abundant GS as support for kefir immobilization during whey fermentation, which is potentially acceptable by industries in order to reduce fast and easily the whey polluting load and produce ethanol.

The purpose of this paper is to study the effect of whey and whey components on the production of dialyzable non‐heme iron – an in vitro indicator of bioavailable forms of the mineral.

Whey, processed whey and whey components were mixed with ferric iron and digested in vitro with pepsin and pancreatin/bile using a dialysis bag containing bicarbonate for pH adjustment. Total and ferrous dialyzable iron were measured and compared to values from protein and non‐protein controls.

Whey produced much more dialyzable iron than egg albumin but less than deproteinized whey. Most of the iron was ferric. Whey protein concentrate was as effective as egg abumin but whey protein isolate, α‐lactalbumin and β‐lactoglobulin slightly reduced dialyzable iron formation. Milk salts produced more dialyzable iron than whey and about as much as deproteinized whey. The major component of whey producing dialyzable iron was citrate, which competes for iron chelation with the whey protein.

This study demonstrates that whey generates a large amount of dialyzable iron from ferric iron sources and that the iron is primarily due to chelation with citrate rather than to digestion of whey proteins. However, the effect of citrate is reduced by whey proteins.

Although whey has a high nutritional value, it was regarded as a waste product until new technology enabled its valuable constituents to be recovered and processed into a wide range of products with a great many uses. Susan Hayes BSc, food technologist at the National Dairy Council, describes these developments in processing technology

The main aim of this study is to investigate the whey protein profiles of different commercially available fermented milk drinks that might have been influenced by the growth of probiotics bacteria that have been added according to the claims made by the manufacturer.

The growth and the subsequent effect of probiotics on whey proteins were investigated through the peptide profiles of the hydrolysed whey protein. The profiles of whey proteins in skimmed milk and the four other probiotic fermented milk drinks were obtained by using the FPLC technique. Changes in whey proteins profiles in fermented milks were evaluated by comparing them with those of unfermented skimmed milk (control). The four samples were those of Yakult, Actimel, Muller and Tesco probiotic cranberry drinks.

This work has shown that all samples demonstrated a degree of protein hydrolysis. The high level of hydrolysis in “Yakult” and “Actimel” drink samples might have been due to the nature of the process, the length of time of fermentation or the high level of proteolytic activities of the micro‐organisms used. When compared with casein, it seems that whey proteins are more resistant to hydrolysis. The results also indicated that only traces of α‐lactalbumin were left in the whey sample from “Yakult” drink. There were noticeable reductions in the other three samples. Orotic acid, on the other hand, showed a decrease in their concentration in all whey protein samples when compared with the skimmed milk sample, except for the “Actimel” sample, which showed a noticeable increase.

This work has shown that there were distinct differences between the control sample (skimmed milk) and the four commercially available probiotic milk‐based fermented health drinks when a direct comparison was carried out between these samples.

The purpose of this paper is to investigate the effects of the components of whey‐protein based aqueous polymer‐isocyanate (API) adhesives on the bond strength.

The bond test (according to the JIS K6806‐2003 standard), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were used to characterise the whey‐protein based API adhesives with various formulations and processing technologies.

The good bond strength of the optimised whey‐protein based API adhesive was attributed to the formation of strong chemical bonds in the bondline and to the additions of polyisocyanate, polyvinyl alcohol (PVA) and nano‐CaCO₃ powder that improved adhesive cohesive strength by either chemical crosslinks or mechanical interlocking. The blending procedures of whey protein, PVA, polyvinyl acetate (PVAc) and p‐p‐MDI had great impacts on the performances of the whey‐protein based API adhesives.

SEM micrographs showed that the effects of blending processes on the bond strength, pot life and colour might be attributed to the particle size of hydrophobic p‐MDI droplet and p‐MDI distribution in the protein‐PVA matrix.

The study lays the foundations of the formulation design and the processing technology for preparing whey‐protein based API adhesives.

The effects of the components of whey‐protein based API adhesives and the effects of blending processes on the bond strength were investigated by means bond strength evaluation, FTIR and SEM analyses; whey protein is utilised successfully to prepare novel API adhesives for structural uses.

The purpose of this paper is to review the advanced technologies and approaches for utilization of waste generated in dairy industry. Whey is highly contaminated, with a high organic load around 100,000 mg O₂/L COD (chemical oxygen demand), and is not used for further processing. The waste generated in different food industries can be utilized in different value addition product with the help of advanced technology.

Major well-known bibliometric information sources are the Web of Science, Scopus, Mendeley and Google Scholar. Several keywords like nutrition value of whey, whey utilization, whey valorization, whey technologies, whey beverages, fruit-based whey beverage, carbonated beverage, probiotic or alcoholic beverages, herbal beverage, fermented beverage and current scenarios were chosen to obtain a large range of papers to be analyzed. A final inventory of 126 scientific sources was made after sorting and classifying them according to different criteria based on topic, academic field country of origin and year of publication.

The comprehensive review of different literature, data sources and research papers seeks to find and discuss various sustainable solutions to this huge waste generated from milk industry. The sustainable use of whey for production and conversion in different types of products can uplift the bio-based economy of industries and thereof national/international economy. The recent upsurge in consumer interest for health-promoting products has opened up new vistas for whey beverages and other whey products research and development.

The paper draws out different sustainable characteristics and technology of whey products available in market, as well as potential products to be launched in the market. Interestingly, over the past few years, dairy industries have applied various technologies to process cheese whey and are in search of new products which can be prepared from the by-product. This review discusses on the recent research development of whey valorization with particular reference to technologies used in the addition to their commercial availability and a way forward.

The purpose of this paper is to develop an environmentally safe aqueous polymer‐isocyanate (API) wood adhesive for structural uses with whey protein isolate (WPI) that is a by‐product of cheese making.

The API formulations with whey proteins denatured at different heating temperatures and times, WPI/polyvinyl alcohol (PVA) denaturing processes, PVA contents and nano‐CaCO₃ (as filler) contents were investigated and optimised according to the JIS K6806‐2003 standard.

A whey‐protein based API adhesive was developed which had 28 h boiling‐dry‐boiling wet compression shear strength 6.81 MPa and dry compression shear strength 13.38 MPa beyond the required values (5.88 and 9.81 MPa, respectively) for structural use of commercial standards. The study also indicated that the thermal denaturation of 40 per cent WPI solution at 60‐63°C could unfold the globular structure of whey protein to some extent and therefore improve the bond strength and bond durability of whey‐protein based API adhesive; the additions of PVA and nano‐CaCO₃ as filler had a significant effect on the bond strength and bond durability of whey‐protein based API adhesive.

The thermally denatured WPI solutions (40 wt%) incline towards being decayed by moulds if not properly formulated.

Owing to the good bond strength and durability and environmental safety, the optimised whey‐protein based API adhesives have greater potential for commercial applications, especially for the structural wood bonds.

A novel API wood adhesive for structural use was developed using whey proteins that are often regarded as a waste due to their relatively small molecules and compact globular structures.

The present study was carried out to standardize the method for preparation of naturally carbonated fermented paneer whey beverage by incorporating pineapple and strawberry fruit juice and to check their suitability in the beverage by evaluating the organoleptic characteristics and shelf life of product.

Beverage was inoculated with yeast culture Clavispora lucitaniae at 0.5 per cent v/v and fermented at 35 ± 1°C for 36 h aerobically. Standardization of total soluble solids (TSS) (16, 15, 14, 13 and 12oBrix) and juice concentration (15, 20, 25 and 30 per cent) of beverage was done on the basis of organoleptic evaluation, and the beverage with TSS 12oB and 30 per cent juice was selected best for further storage study. Two types of beverages were prepared: paneer whey beverage blended with pineapple juice and paneer whey beverage blended with strawberry juice, and were stored at refrigerated (4 ± 1oC) and ambient (25 ± 5oC) conditions. Effect of storage on physico-chemical, microbiological and sensory attributes were studied periodically after every 15 days for 90 days of storage period.

There was significant decrease in brix:acid ratio (p = 0.0008) from 12.0 to 9.3, total sugar (p = 0.017) from 10.8 to 6.8, ascorbic acid (p = 0.002) from 17.8 to 9.3 mg/100 mL and lactose (p = 0.037) from 3.1 to 0.6 per cent content over 90 days of ambient storage period. Total yeast count increased during the initial stages of fermentation and started declining after 60 days of storage. The alcohol production started after 15 days and reached 0.7 per cent after 90 days for paneer whey beverages blended with strawberry juice. The more variations were found in the physico-chemical and microbiological properties of the beverage at ambient storage than refrigeration storage. Highest score for color, flavor, mouthfeel and overall acceptability was found on third days, which decreased further during the storage. The comparative study of the paneer whey beverage blended with strawberry juice stored at ambient and refrigeration temperature showed that maximum decrease was found for score of appearance/color, flavor, mouthfeel and overall acceptability at ambient temperature as compared to refrigeration temperature. Beverage stored at refrigeration temperature was found more acceptable than the beverage which was stored at ambient temperature irrespective of all types of beverages.

The refrigerated beverage was found more acceptable up to 90 days, whereas beverage stored under ambient conditions was found acceptable up to 60 days. The products so obtained had naturally produced CO₂, and little alcohol content added effervescence, sparkle, tangy taste and flavoring characteristics.

Whey is a by-product of paneer, cheese and casein industry and considered as a dairy waste. Worldwide, approximately 180–1,900 million tons of whey is produced annually. Whey is classified as a high pollutant due to its organic matter level. Owing to its high chemical oxygen demand and biological oxygen demand, it is a big threat to the environment. Whey contains 4.5%–5.0% lactose, 0.6%–0.7% protein, 0.4%–0.5% lipids, vitamins and minerals. Due to its high nutritional profile, it is a good substrate for the microorganisms for production of natural pigments. The purpose of this paper is to review the utilization of low cost substrate (whey) for production of various types of pigments and their applications in different sectors.

The databases for the search included: Scopus, PubMed, Science Direct, Web of Science, Research gate and Google. The main search was directed towards different types of natural pigments, stability, technologies for enhancing their production and contribution towards circular economy. Approximately 100 research papers were initially screened. A global search was conducted about natural pigments. Research articles, review papers, books, articles in press and book chapters were the type of search for writing this review paper.

Production of natural pigments using whey and their addition in food products not only improves the colour of food but also enhances the antioxidant properties of food products, helping the health benefits by chelating free radicals from the body. The sustainable use of whey for production of natural pigments can improve the bio-based economy of different industries and thereof the national economy.

Efficient utilization of whey can bring a lot more opportunities for production of natural pigments in a sustainable manner. The sustainable approach and circular economy concepts will benefit the dependent industries and health conscious consumers. The potential uses of whey for the production of natural pigments using diverse organisms are highlighted in this paper.

Background/Objectives: Protein-based meal replacements (MR) with viscous soluble fibre are known aids for weight loss. This study aims to compare the effects of new whey and vegan MR containing different amounts of PGX (PolyGlycopleX) on weight loss over 12 weeks, along with a calorie-restricted diet.

Subjects/Methods: Sixty-eight healthy adults of both sexes (53 women; 15 men; average age 47.1 years; BMI 31 ± 7.1 kg/m² and weight 85.05 ± 23.3 kg) were recruited. Participants consumed a whey or vegan MR twice/d (5–10 g/day PGX) with a low-energy diet (1,200 kcal/day), over 12 weeks. Weight, height, waist and hip circumference were recorded (four time periods).

Results: Forty-four participants completed the study. Results showed significant reductions in average body weight and at week 12, whey group was [−7.7 kg ± 0.9 (8.3%), p < 0.001] and vegan group was [−4.5 kg ± 0.8 (6.2%), p < 0.001)]. All participants (n = 44; BMI 27 to 33 kg/m²) achieved significant reductions in body measurements from baseline to week 12; p < 0.001. Conclusions: Supplementation of protein-based MR with PGX and a balanced, low-energy diet, appears to be an effective approach for short-term weight loss.

As the authors were evaluating if the MR as a whole (i.e. with PGX) caused weight loss from baseline over the 12 weeks, no comparators, i.e. just the MR without PGX, were used. Formulation of these new MRs resulted in a whey product with 5 g PGX and a vegan product with 2.5 g PGX. Only 2.5 g PGX could be formulated with the vegan protein due to taste and viscosity limitations. Study participants were not randomized and no control groups (e.g. no MR or MR without PGX but with energy restricted diet) were used. Furthermore, it is not clear whether the sort of protein alone or the combination with a higher amount of PGX (whey with 5 g PGX/serving vs vegan with 2.5 g PGX/serving) has contributed to these significant greater weight-loss effects. This was something the authors were testing, i.e. could only 2.5 g PGX/serving have an effect on weight loss for a vegan MR. These limitations would be somethings to evaluate in a subsequent randomized controlled study. Hence, the results of this study may serve as a good starting point for further sophisticated randomized controlled trials that can demonstrate causality – which the authors acknowledge as one of the fundamental limitations of an observational study design. Participants tracked their calories but adherence and compliance were self-assessed and they were encouraged to keep their exercise routine consistent throughout the study. Hence, these are further limitations. No control group was used in this study to observe the effect of the dietary intervention and/or physical activity on weight loss alone. However, a goal of the authors was to keep this study as close to a real-life situation as possible, where people would not be doing any of these measurements, to see if with minimal supervision or intervention, people can still lose weight and alter their body composition. Furthermore, differences in gender and the corresponding weight loss effects in response to MR-protein-based treatments could be evaluated in follow-up studies.

This study indicates that the consumption of protein-based (animal, whey or plant, pea protein) MR incorporating the highly soluble viscous PGX is beneficial for weight loss when combined with a healthy-balanced, calorie-restricted diet. MRs at either 2.5 g or 5 g per serving (RealEasy^TM with PGX) proved to be a highly effective as a short-term solution for weight loss. The observed results are encouraging, however, further long-term studies (i.e. randomized clinical trials RCT) are needed to confirm the clinical relevance. RCTs should focus on the individual effects of PGX and/or the different protein sources used in MRs, on weight loss and the maintenance of the reduced body weight, and should measure detailed blood parameters (lipid profiles, glucose etc.) as well as collect detailed exercise and food consumption diaries.

To the authors’ knowledge, this is the first study comparing a whey versus vegan, (as pea) protein-based MR that is supplemented with fibre PGX; thus, this work adds information to the already existing literature on fibre (such as PGX) and MRs regarding their combined weight loss effects. The purpose of this study was to observe if the novel protein-based (either whey or vegan versions) MR RealEasy^TM with PGX at 2.5 or 5 g in addition to a calorie-restricted diet (total of 1,200 kcal/day) would aid in weight loss in individuals over a 12-weeks period. Adding increasing amounts of whey protein and soluble fibre can help reduce subsequent ad libitum energy intake which could help adherence to energy restricted diets, but whether similar effects are seen with vegan protein is unclear – this study does aim to address this.