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Article
Publication date: 28 October 2019

Yahiaoui Zidan, Sherazede Bouderbala, Cherrad Hayet and Bouchenak Malika

The purpose of this study is to determine the effect of olive cake (OC) on lipid peroxidation as well as antioxidant enzymes activities of serum, red blood cells (RBCs…

Abstract

Purpose

The purpose of this study is to determine the effect of olive cake (OC) on lipid peroxidation as well as antioxidant enzymes activities of serum, red blood cells (RBCs) and liver, in streptozotocin (STZ)-induced-diabetic rat fed cholesterol-enriched diet.

Design/methodology/approach

Hypercholesterolemic male rats were rendered diabetic (HC-D) by a single intraperitoneal injection dose of STZ (35 mg/kg BW). HC-D rats were divided into two groups fed for 28d a diet supplemented with OC at 7.5 percent (HC-D-OC) or not (HC-D). A control group (C) was submitted to standard diet containing 20 per cent casein for the same experimental period.

Findings

RBCs, serum and liver thiobarbituric acid reactive substances (TBARS) contents were significantly increased in HC-D, compared to C group (p = 0.04, p = 0.02 and 0.03). These values were significantly decreased (48 per cent and 64 per cent; p = 0.02 and p = 0.0007) in serum and liver of HC-D-OC vs HC-D group. In RBCs, superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) activities were, respectively, 1.5, 2- and 1.7-fold higher (p = 0.03, p = 0.008 and p = 0.03) in HC-D group compared to HC group. In serum and liver, SOD, CAT and GST activities were, respectively, 1.3-, 2.6- and 1.6-fold increased (p = 0.03, p = 0.007 and p = 0.02). In HC-D-OC compared to HC-D group, RBCs glutathione peroxidase (GSH-Px), CAT and GST activities were, respectively, 2.1-, 3.3- and 2.1-fold higher (p = 0.04, p = 0.0009 and p = 0.03). In serum, SOD and CAT activities were, respectively, 1.5- and 1.9-fold increased (p = 0.02, p = 0.02). In liver, SOD, GSH-PX, CAT and GST activities were significantly increased (p = 0.005, p = 0.03, p = 0.02 and p = 0.04).

Originality/value

In diabetic rats-fed cholesterol-enriched diet, OC was able to reduce oxidative stress by decreasing lipid peroxidation and increasing antioxidant enzymes activities in serum, RBCs and liver.

Details

Nutrition & Food Science , vol. 50 no. 4
Type: Research Article
ISSN: 0034-6659

Keywords

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Article
Publication date: 19 August 2019

Hayet Cherrad, Sherazede Bouderbala, Yahiaoui Zidan and Djamil Krouf

The purpose of this study is to determine the effect of olive cake (CO) on glycaemia and lipemia and lipid peroxidation and antioxidant enzymes activities in erythrocytes…

Abstract

Purpose

The purpose of this study is to determine the effect of olive cake (CO) on glycaemia and lipemia and lipid peroxidation and antioxidant enzymes activities in erythrocytes and tissues, in streptozotocin (STZ)-induced diabetic rats.

Design/methodology/approach

Diabetes was induced by a single intraperitoneal injection of STZ (55 mg/kg BW). In total, 12 diabetic D rats, weighing 260 ± 20 g, were divided into two groups fed a casein diet supplemented (D-OC) or not (D) with OC (7.5 per cent), for four weeks.

Findings

In D-OC compared with D, glycaemia, total cholesterol and triglycerides values (−40 per cent; p = 0.007, 27 per cent; p = 0.007 and −27 per cent; p = 0.0019). In erythrocyte, liver, kidney, heart, muscle and brain, thiobarbituric acid reactive substances contents were respectively, (−19 per cent; p = 0.03, −32 per cent; p = 0.002, −20 per cent; p = 0.04, −68 per cent; p = 0.003, −74 per cent; p = 0.0003 and −38 per cent; p = 0.04). In erythrocyte, SOD, GSH-Px and CAT activities were respectively, (+14 per cent; p = 0.01, +74 per cent; p = 0.012 and +34 per cent; p = 0.0009). In the liver, kidney, heart and muscle, SOD activity was respectively, (+31 per cent; p = 0.004, +12 per cent; p = 0.038, +43 per cent; p = 0.001 and +23 per cent; p = 0.18). GSH-Px activity was respectively, (+121 per cent; p = 0.0009, 89 per cent; p = 0.0006, + 95 per cent; p = 0.008, +71 per cent; p = 0.02 and +26 per cent; p = 0.01), in the liver, kidney, heart, muscle and brain. Catalase activity was (+21 per cent; p = 0.008) in the liver, (+88 per cent; p = 0.0002 in the kidney, +53 per cent; p = 0,002 in the heart and 83 per cent; p = 0.00004 in the muscle).

Originality/value

In diabetic rats, OC reduces hyperglycaemia induced by STZ and attenuates triglyceridemia and cholesterolemia. This residue is able to decrease the oxidative stress by increasing the antioxidant enzymes activity in erythrocytes and tissues. The high contents of phytoconstituents present in OC are considered to be responsible for this effect.

Details

Nutrition & Food Science , vol. 50 no. 2
Type: Research Article
ISSN: 0034-6659

Keywords

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