Search results

1 – 2 of 2
To view the access options for this content please click here
Article
Publication date: 1 September 2004

TeckLok Wong, Megan Louise Devane, John Andrew Hudson, Paula Scholes, Marion Grace Savill and John D. Klena

This study describes a multiplex polymerase chain reaction (PCR) detection system combined with enrichment growth conditions for simultaneous detection and identification…

Abstract

This study describes a multiplex polymerase chain reaction (PCR) detection system combined with enrichment growth conditions for simultaneous detection and identification of C. jejuni, C. coli and thermotolerant Campylobacter in poultry pack rinses. The PCR primers were tested against a range of Campylobacter and non‐Campylobacter species, and PCR products were only amplified from target organisms. The sensitivity of the method was similar to that obtained by conventional plating procedures, but when used in combination with the MPN method of enumeration could detect levels down to 6 MPN/100 ml of rinse. The validation of 50 samples of chicken pack rinses demonstrated the versatility of this approach in microbiological surveys to yield data for risk assessments.

Details

British Food Journal, vol. 106 no. 9
Type: Research Article
ISSN: 0007-070X

Keywords

To view the access options for this content please click here
Article
Publication date: 1 September 2004

TeckLok Wong, Rosemary Janet Whyte, Angela Joyce Cornelius and John Andrew Hudson

Transmission routes that may contribute to the overall picture of campylobacteriosis and salmonellosis were explored by analysing the outside surfaces of 300 retail raw…

Abstract

Transmission routes that may contribute to the overall picture of campylobacteriosis and salmonellosis were explored by analysing the outside surfaces of 300 retail raw chicken packs for prevalence and enumeration of Campylobacter using a MPN/PCR assay and Salmonella using a MPN/visual immunoassay procedure. The surfaces of 72 packs (24.0 per cent) were externally contaminated with C. jejuni; 32 with counts of <6 MPN/pack and the remaining 40 with counts ranging from 6 to >2,200 MPN/pack. One whole chicken sample (0.3 per cent) was contaminated with S. Tennessee at <6 MPN/pack. The surfaces of offal packs were most contaminated with Campylobacter, with 18 per cent having a count of ≥100 MPN/pack in comparison with chicken portion packs (4/200, 2 per cent) and whole bird packs (1/50, 2 per cent). These observations suggest that packs could be a source of cross‐contamination, however, the contribution of this outer surface contamination pathway to foodborne illness can only be properly determined by development of a validated risk assessment model.

Details

British Food Journal, vol. 106 no. 9
Type: Research Article
ISSN: 0007-070X

Keywords

1 – 2 of 2