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Given the limited understanding of the process of transgenerational entrepreneurship and that knowledge is a fundamental antecedent of entrepreneurial endeavors, this study aims to shed light on how entrepreneurial families (EFs) nurture entrepreneurship across generations, which knowledge is required within the EF to spur new entrepreneurial activities, and how is this knowledge acquired.

Considering the paucity of empirical evidence on the topic under investigation and the related exploratory nature of this study, the authors adopted a qualitative approach by conducting a case study on an Italian wine-making family business.

The case analysis reveals that EF members are required to acquire different types of knowledge at different generational stages to spur new entrepreneurial activities, specifically technical knowledge in the second generation and business knowledge in the third generation. Moreover, the data analysis shows two mechanisms, namely, trust among generations and role separation, that, during both generational transitions, enabled and empowered the younger generations to exploit their knowledge to explore entrepreneurial opportunities and engage in new entrepreneurial activities.

This study provides novel insights into the role of knowledge in transgenerational entrepreneurship, particularly looking at knowledge acquired by EF members across generations. Accordingly, this research contributes to the literature streams of transgenerational entrepreneurship, knowledge management in family businesses and broader knowledge management research.