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1 – 6 of 6Hadjera Chekkal, Nour el Imane Harrat, Fatima Bensalah, Fouad Affane, Sabrine Louala and Myriem Lamri-Senhadji
The effects of Opuntia ficus indica (OFI) cladodes on uricemia level, endothelial dysfunction and oxidative damage were studied in young rats fed a cafeteria diet (CD).
Abstract
Purpose
The effects of Opuntia ficus indica (OFI) cladodes on uricemia level, endothelial dysfunction and oxidative damage were studied in young rats fed a cafeteria diet (CD).
Design/methodology/approach
A total of 16 young male Wistar rats (weighing 110 ± 20 g and four weeks old) were divided into two homogenous groups. The first group received a CD containing 50% of hyperlipidic diet and 50% of junk food mix (processed mix: hyper-fat, hyper-salted and sweetened) (CD group), and the second group (CD + OFI nopalitos) received the same diet supplemented with 50 g of fresh OFI nopalitos (young cladodes) for 30 days.
Findings
OFI nopalitos regulate the hyperuricemia, improve the endothelial dysfunction by raising the bioavailability of nitric oxide(NO) and reduce prooxidant markers by reducing lipid peroxidation and protein oxidation (p < 0.05) and boosting antioxidant capacity and enhancing the antioxidant enzymes activities (p < 0.05) in blood and aorta tissues of rats early fed with a high-fat diet /junk food.
Social implications
By-products of OFI have specific functional properties that may be beneficial in metabolic disorders and offer a better alternative with an economic and sustainable development perspective.
Originality/value
By-products of OFI highlight potential functional properties mainly based on its potent antioxidant capacity. By-products of OFI can be used as a promising nutraceutical resource to prevent various metabolic disorders in relation with cardiovascular diseases or hyperuricemia in subjects consuming junk food and or living in the Western society to reach the objectives of health policy and maintain a sustainable health system development.
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Sherazed Hamza-Reguig, Nabila Boukhari Benahmed Daidj, Sabrine Louala, Ahmed Boualga and Myriem Lamri-Senhadji
The purpose of this study was to investigate the impact of replacing two different fats on dyslipidemia, glycemic balance and adipose tissue redox status in obese rats.
Abstract
Purpose
The purpose of this study was to investigate the impact of replacing two different fats on dyslipidemia, glycemic balance and adipose tissue redox status in obese rats.
Design/methodology/approach
Obesity was induced by feeding a high-mutton-fat diet during three months. An experimental group (n = 24) was divided into two groups that were fed during one month, 20 per cent of margarine or sardine oil. At Day 30, six rats from each group were sacrificed and the remaining rats were then subjected to a change in diet for one month: margarine was replaced by sardine oil and inversely, and then the rats were sacrificed. Three other groups (n = 6), each fed during two months, 20 per cent of margarine, sardine oil or mutton fat, served as controls.
Findings
Substitution of sardine oil by margarine compared to control sardine oil had increased triacylglycerols (TGs), glycosylated hemoglobin (HbA1c) and isoprostanes (IsoPs) values, but decreased thiobarbituric acid reactive substances (TBARS) and superoxide dismutase activity. Replacing margarine by sardine oil compared to control margarine reduced total cholesterol, TG, HbA1c, TBARS and IsoP contents but enhanced glutathione reductase and peroxidase activities. Nevertheless, comparing with the mutton fat, the two substitutions had improved glycemic and lipidic abnormalities and attenuated lipoperoxidation by enhancing enzymatic antioxidant defense. These favorable effects were better when margarine was replaced by sardine oil.
Originality/value
Substituting margarine with sardine oil seems to attenuate beneficial cardiometabolic risk markers associated to obesity and potentiate efficiency adipose tissue against the oxidative stress induced by the obesogenic diet.
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Nour el Imane Harrat, Sabrine Louala, Fatima Bensalah, Fouad Affane, Hadjera Chekkal and Myriem Lamri-Senhadji
The purpose of this study was to investigate the effects of prickly pear (Opuntia ficus indica (OFI)) nopalitos on body weight, food consumption, arterial blood pressure, glucidic…
Abstract
Purpose
The purpose of this study was to investigate the effects of prickly pear (Opuntia ficus indica (OFI)) nopalitos on body weight, food consumption, arterial blood pressure, glucidic homeostasis, cholesterol metabolic pathway and tissues redox status in type 2 diabetic (T2D) rats fed a high-fat diet (HFD).
Design/methodology/approach
Rats were fed by a HFD containing 30 per cent sheep fat for 10 weeks, after which they were rendered diabetic by an injection of a low dose of streptozotocin (STZ) (35 mg/kg). The diabetic rats were then divided into two groups. The first group consumed the HFD supplemented with 5 per cent (g/100 g diet) of freeze-dried OFI nopalitos (HFD-OFI), and the second group received the HFD without supplementation (HFD).
Findings
OFI nopalitos treatment decreased significantly arterial diastolic (−20%; p = 0.0001) and systolic (−16%; p = 0.0001) pressures, glycemia (−14%; p = 0.03), insulinemia (−50%; p = 0.04), glycated hemoglobin (−49%; p = 0.003), homeostasis model assessment insulin resistance (−67%; p = 0.03), cholesterolemia (−31%; p = 0.003), very-low and low-density lipoprotein cholesterol (−38%; p = 0.002 and −63% p = 0.0002, respectively); thiobarbituric acid reactive substances and lipid hydroperoxide contents, respectively, in liver (−26% p = 0.02, −20% p = 0.02), adipose tissue (−30% p = 0.002, −25% p = 0.001), muscle (−29% p = 0.003, −25% p = 0.008) and kidney (lipid hydroperoxides only (−28%; p = 0.001) but increased high-density lipoprotein (HDL2) cholesteryl esters (+61%; p = 0.0001), serum lecithin: cholesterol acyltransferase activity (+21%; p = 0.006) and antioxidant enzymes activities (superoxide dismutase, glutathione peroxidase and catalase) of some tissues (liver, adipose tissue, muscle and kidney).
Originality/value
Freeze-dried OFI nopalitos improves arterial blood pressure, glycemic control, metabolic pathway of cholesterol and redox status in T2D rats.
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Hadjera Chekkal, Nour el Imane Harrat, Fouad Affane, Fatima Bensalah, Sabrine Louala and Myriem Lamri-Senhadji
This paper aims to evaluate the protective potential of prickly pear cactus fresh cladodes (opuntia ficus indica (OFI)) on glycemic disorders, dyslipidemia, prooxidant/antioxidant…
Abstract
Purpose
This paper aims to evaluate the protective potential of prickly pear cactus fresh cladodes (opuntia ficus indica (OFI)) on glycemic disorders, dyslipidemia, prooxidant/antioxidant stress biomarkers and reverse cholesterol transport (by evaluating the activity of lecithin-cholesterol acyltransferase (LCAT)) and paraoxonase (PON1) in rats prematurely exposed to cafeteria diet (CD).
Design/methodology/approach
Sixteen young rats were divided into two groups fed CD containing 50 per cent of hyperlipidic diet (HLD) and 50 per cent of junk food mix supplemented or not with 50 g of fresh young cladodes of OFI to 100 g of CD, during 30 days.
Findings
OFI cladodes supplementation decreased significantly body weight (p < 0.001), food intake (p < 0.05), adipose tissue weight (p < 0.01), fasting glycemia and glycosylated hemoglobin (p < 0.01), homeostasis model assessment (HOMA-IR) and insulinemia (p < 0.001), levels of cholesterol (C) (p < 0.05) and triacylglycerols (TG) (p < 0.01) in serum and in very low-density lipoproteins (VLDL-C p < 0.05 and VLDL-TG p < 0.01) and improves reverse cholesterol transport by increasing high-density lipoprotein cholesteryl-esters concentrations (p < 0.001) and by stimulating LCAT activity. Moreover, they attenuated lipid peroxidation in VLDL and low-density lipoproteins by increasing atheroprotective activity of PON-1 and in liver and adipose tissue by enhancing enzymatic antioxidant defence.
Social implications
The young cladodes of OFI because of their antiobesity benefits could constitute a novel functional ingredient in pharmaceutical and nutraceutical applications.
Originality/value
Young cladodes of OFI in rat precociously submitted to a hyperlipidic diet/junk food (cafeteria model) seem to prevent metabolic disorders associated with obesity.
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Aicha Benyahia‐Mostefaoui, Sabrine Louala and Myriem Lamri‐Senhadji
The present investigation was undertaken to study the potential effects of milk lipids compared to sardine oil on inflammation biomarkers and lipid peroxidation in…
Abstract
Purpose
The present investigation was undertaken to study the potential effects of milk lipids compared to sardine oil on inflammation biomarkers and lipid peroxidation in hypercholesterolemic rats. The paper aims to discuss these issues.
Design/methodology/approach
Male Wistar rats were fed 20 percent casein combined with 5 percent milk lipids or 5 percent sardine oil and 1 percent cholesterol for 28 days. A control group was fed a standard diet.
Findings
No significant difference in serum triacylglycerol (TG) was found in the milk lipids versus sardine oil and control. However, serum TG was reduced (1.7‐fold) with sardine oil compared with the control. Serum total cholesterol (TC) was, respectively, 3.6‐ and 2.5‐fold higher in milk lipids and sardine oil, respectively, compared with control. Compared to sardine oil, TC value was 1.4‐fold higher in the milk lipid. Serum C‐reactive protein (CRP) was elevated (eight‐ and 33‐fold) in the milk lipid and sardine oil compared to control, respectively. However, CRP value was four‐fold lower in milk lipids than those in sardine oil. Compared to sardine oil, iron value was two‐fold higher in milk lipids versus sardine oil. Malondialdehyde content of red blood cell, heart and brain were decreased in milk lipids versus sardine oil (p<0.05). Hydroperoxydes contents in milk lipids were also lower in heart and aorta compared to sardine oil and control (p<0.05).
Originality/value
Milk lipids compared to sardine oil does not modulate the hypercholesterolemia but decreases inflammation biomarkers and seems to protect efficiency of some tissues against the cytotoxic action and oxidative stress of cholesterol enriched diet.
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Sherazed Hamza‐Reguig, Sabrine Louala, Ahmed Boualga and Myriem Y Lamri‐Senhadji
The purpose of this paper was to evaluate the effect of sardine protein on the redox status in rats fed a cholesterol‐rich diet.
Abstract
Purpose
The purpose of this paper was to evaluate the effect of sardine protein on the redox status in rats fed a cholesterol‐rich diet.
Design/methodology/approach
Hypercholesterolemic rats were divided into two groups fed diets enriched with cholesterol and containing 20 percent of sardine proteins (SPc) or casein (CASc) for 28 days. A control group was fed a standard diet (CAS).
Findings
After 28 days of experiment, no significant difference in serum total cholesterol triacylglycerols and uric acid was found with the three diets. Serum albumin content was, respectively, 2‐fold higher in SPc than those in CASc group. Compared to CAS, this value was 1.3‐fold lower in CASc group. In liver and heart, lipid peroxidation was 1.7‐ and 2‐fold lower in SPc compared with CASc and CAS, respectively. In red blood cells and epididymal fat, superoxide dismutase activity was, respectively, 1.3‐and 3‐fold higher in SPc compared to CASc. Epididymal fat and heart catalase activity were, respectively, elevated (+50 and +79 percent) in SPc than in CASc. Sardine protein decreased nitric oxide levels in heart and epididymal fat (twofold) compared to CASc but compared to control group, nitric oxide value was higher in epididymal fat (2‐fold) and liver (3‐fold).
Originality/value
Sardine protein exerts a beneficial action against oxidative stress caused by dietary cholesterol specifically in the heart by reducing lipid peroxidation and enhancing catalase activity.
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