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The purpose of this paper is, first, to evaluate the quality of commonly consumed fish species in Fez region (Morocco) by quantifying the levels of histamine in fresh fish samples using competitive enzyme-linked immune sorbent assay (ELISA), and then to study the effect of heating and enzymatic digestion on the level of fish-histamine.

Histamine content was tested on 80 fresh fish samples of 11 species collected from various local stores in Fez region, from February to March 2016. The analyses were performed using a competitive ELISA assay to measure histamine in fish samples.

ELISA results showed that 80 percent of 80 fish samples analyzed was found to contain much lower levels of histamine (<1 mg/kg) and can be considered to be safe for human consumption. However, 20 percent of the samples (16/80) had histamine level higher than the tolerance limit of 200 mg/kg established by Moroccan and International regulations, which the maximum level reached up to 7,331 mg/kg in horse mackerel. Concerning the effect of heating and enzymatic treatments on fish-histamine levels, results have shown a low reduction in histamine contents in the majority of fish samples under these treatments.

In conclusion, good quality of the fish product, demonstrated by histamine levels, was found in the most of fish samples analyzed, while 20 percent of the samples are non-compliant and exceed the tolerance limit established by the national and international regulatory limit.

The purpose of this study is to compare the evolution of self-reported milk allergy in children and adults within the population of Fez-Meknes region, as well as to investigate the consumption of milk and its correlation to milk allergy.

It is a cross-sectional study conducted in Fez-Meknes region between April 2014 and March 2015 basing on a questionnaire completed by 3,068 children and 1,281 adults.

The results showed that food allergy was more reported among children (29.4 per cent) than adults (16.9 per cent). Milk allergy was reported by 5.3 per cent of children and 4.2 per cent of adults. Children reported mostly cutaneous manifestations (64.8 per cent) while adults reported mostly gastrointestinal manifestations (68.5 per cent). The consumption of milk showed a protective effect against the development of milk allergy (p < 0.001).

The consumption of milk was associated significantly with a low rate of milk allergy, especially in adults. However, the pasteurization of milk seemed to be correlated to increase milk sensitivity.

This study aims to evaluate the effect of heating and pepsin hydrolysis on the allergenicity of purified cow’s milk ß-lactoglobulin using individual sera from Moroccan population.

A cross-sectional study was carried out in Hospitals of Fez-Meknes region, to evaluate specific IgE to raw cow milk, as well as to heated and pepsin-hydrolyzed β-lactoglobulin.

Results showed that 6.6 per cent of studied patients presented high values of serial IgE. The effect of treatments on the allergenicity of ß-lactoglobulin showed that heating at 90°C and pepsin hydrolysis, for 1 h each, showed an important decrease in the recognition of human IgE with a maximum of reduction of 81 and 91 per cent, respectively.

This reduction of immunoreactivity of human IgE to treated ß-lactoglobulin suggested that this Moroccan population recognized mostly conformational epitopes.

This paper aims to examine the differences in the self-reported allergies to food, especially fish and shellfish, between children and adults, and to study the association between fish consumption and self-reported fish and shellfish allergy (FSA) according to age.

This study is based on a survey conducted between April 2014 and December 2016 where children and adults were recruited from the general population of the Fez-Meknes region. The total studied population was 4,046 people.

The results show that the self-reported food allergy (FA) of the general population was more frequent among children (28.2 per cent) than adults (16.8 per cent), in which eggs, fish/shellfish, milk and cereals were the most common food cited. With regard to the self-reported FSA, the authors found a prevalence of 9.5 per cent in both children and adults, whereas fish species allergies were more frequent among adults than children. The most common clinical manifestations observed in this population were cutaneous reactions. The study of the association between fish consumption and FSA shows that people who consumed fish had a lower rate of FSA, especially in adults (p < 0.001).

The self-reported FA of the general population was more pronounced in children than adults. Regarding the self-reported FSA, the authors have shown a prevalence of 9.5 per cent in both children and adults, indicating an important sensitivity of their population to fish and shellfish. The authors have shown that consumption of fish was associated significantly with a lower rate of FSA, especially in adults.