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The purpose of this study is to design and characterize the dielectrophoretic (DEP) microelectrodes with various array structure arrangements in order to produce optimum non-uniform electric field for particle capture. The DEP-electrodes with 2D electrode structure was fabricated and characterized to see the effect of electrode structure configuration on the capture capability of the cells suspending in the solution.

The presented microelectrode array structures are made of planar conductive metal structure having same size and geometry. Dielectrophoretic force (F_DEP) generated in the fluidic medium is initially simulated using COMSOL Multi-physics performed on two microelectrodes poles, which is then continued on three-pole microelectrodes. The proposed design is fabricated using standard MEMS fabrication process. Furthermore, the effect of different sinusoidal signals of 5, 10 and 15 volt peak to peak voltage (V_pp) at fixed frequency of 1.5 MHz on capturing efficiency of microelectrodes were also investigated using graphite metalloids particles as the suspended particles in the medium. The graphite particles that are captured at the microelectrode edges are characterized over a given time period.

Based on analysis, the capturing efficiency of microelectrodes at the microelectrode edges is increased as voltage input increases, confirming its dependency to the F_DEP strength and direction of non-uniform electric field. This dependency to field consequently increases the surface area of the accumulated graphite. It is also showed that the minimum ratio of the surface accumulated area of captured graphite is 1, 2.75 and 9 μm2 for 5, 10 and 15 V_pp, respectively. The simulation result also indicates a significant improvement on the performance of microelectrodes by implementing third pole in the design. The third pole effect the particles in the medium by creating stronger non-uniform electric field as well as more selective force toward the microelectrodes’ edges.

The microelectrode array arrangement is found as a reliable method to increase the strength and selectivity of non-uniform electric field distribution that affect F_DEP. The presented findings are verified through experimental test and simulation results.

This paper aims to present the dielectrophoresis (DEP) force (FDEP), defined as microelectrofluidics mechanism capabilities in performing selective detection and rapid manipulation of blood components such as red blood cells (RBC) and platelets. The purpose of this investigation is to understand FDEP correlation to the variation of dynamic dielectric properties of cells under an applied voltage bias.

In this paper, tapered design DEP microelectrodes are used and explained. To perform the characterization and optimization by analysing the DEP polarization factor, the change in dynamic dielectric properties of blood components are observed according to the crossover frequency (f_xo) and adjustment frequency (f_adj) variation for selective detection and rapid manipulation.

Experimental observation of dynamic dielectric properties change shows clear correlation to DEP polarization factor when performing selective detection and rapid manipulation. These tapered DEP microelectrodes demonstrate an in situ DEP patterning efficiency more than 95%.

The capabilities of tapered DEP microelectrode devices are introduced in this paper. However, they are not yet mature in medical research studies for various purposes such as identifying cells and bio-molecules for detection, isolation and manipulation application. This is because of biological property variations that require further DEP characterization and optimization.

The introduction of microelectrofluidics using DEP microelectrodes operate by selective detecting and rapid manipulating via lateral and vertical forces. This can be implemented on precision health-care development for lab-on-a-chip application in microfluidic diagnostic and prognostic devices.

This study introduces a new concept to understand the dynamic dielectric properties change. This is useful for rapid, label free and precise methods to conduct selective detection and rapid manipulation of mixtures of RBC and platelets. Further, potential applications that can be considered are for protein, toxin, cancer cell and bacteria detections and manipulation. Implementation of tapered DEP microelectrodes can be used based on the understanding of dynamic dielectric properties of polarization factor analysis.

The purpose of this paper is to highlight a novel manufacturing process for a biochip with a multi‐electrode array (MEA) that is specifically designed for use in characterising cardio‐active substances and to demonstrate a novel proposed solution prototype that has been constructed to meet the needs of end‐users.

Practical problems encountered with conventional MEA biochips are described and a novel biochip design to tackle these problems is presented. The manufacturing approach used to produce the prototypes of that design is described and depicted.

The novel prototype MEA biochips were successfully manufactured using conventional electronics manufacturing approaches. Prototypes demonstrated limited successes in the early stages of testing. Further revisions of the feature geometry are required to implement an alternative MEA biochip that is suitably reliable.

Basic photolithography techniques have been used to construct a base substrate for proof‐of‐principle studies. Increased sophistication in manufacturing stages is required in future iterations of the proposed concept.

This paper introduces a problem encountered by MEA system adopters that requires a suitable solution. The scale up of an electronics manufacturing process‐based solution to the problems described holds much promise for the screening of new chemical entities.

Olfaction plays a very important role in daily life. The olfactory system has the ability to recognize, discriminate and identify thousands of odorant compounds with extremely high sensitivity and specificity. The research on olfactory system has very important values in exploring the mechanisms of information processing in the other sensory nervous systems and brain. Recently, with the development of molecular biological and microelectronics technology research, the study of olfactory cell-based sensors has made great progress. The purpose of this paper is to provide details of recent developments in olfactory cell-based sensors.

Following an introduction, this paper first discusses some olfactory cell-based biosensors, which focus on the light-addressable potentiometric sensors and the microelectrode arrays. Second, surface modification, microfabrication and microfluidic technology which can improve the efficiency of cell immobilization will be summarized. The research trends of olfactory cell-based sensor in future will be proposed.

This paper shows that the biosensors’ performance is expected to be greatly improved due to the fast development of nanotechnology, optical technology and microelectronics. More and more emerging intelligent olfactory sensors will have a promising prospect in many application fields, including food quality and safety assessment, environmental monitor and human diseases detection.

This paper provides a detailed and timely review of the rapidly growing research in the olfactory cell-based sensors.

The purpose of this paper is to visualize protein manipulation using dielectrophoresis (DEP) as a substantial perspective on being an effective protein analysis and biosensor method as DEP is able to be used as a means for manipulation, fractionation, pre-concentration and separation. This research aims to quantify DEP using an electrochemical technique known as cyclic voltammetry (CV), as albumin is non-visible without any fluorescent probe or dye.

The principles of DEP were generated by an electric field on tapered DEP microelectrodes. The principle of CV was analysed using different concentrations of albumin on a screen-printed carbon electrode. Using preliminary data from both DEP and CV methods as a future prospect for the integration of both techniques to do electrical quantification of DEP forces.

The size of the albumin is known to be 0.027 µm. Engineered polystyrene particle of size 0.05 µm was selected to mimic the DEP actuation of albumin. Positive DEP of the sample engineered polystyrene particle was able to be visualized clearly at 10 MHz supplied with 20 Vpp. However, negative DEP was not able to be visualized because of the limitation of the apparatus. However, albumin was not able to be visualized under the fluorescent microscope because of its translucent properties. Thus, a method of electrical quantification known as the CV technique is used. The detection of bovine serum albumin (BSA) using the CV method is successful. As the concentration of BSA increases, the peak current obtained from the voltammogram decreases. The peak current can be an indicator of DEP response as it correlates to the adsorption of the protein onto the electrodes. The importance of the results from both CV and DEP shows that the integration of both techniques is possible.

The integration of both methods could give rise to a new technique with precision to be implemented into the dialyzers used in renal haemodialysis treatment for manipulation and sensing of protein albumin.

This paper aims to provide a fabrication and measurement of a highly stretchable pressure sensor with a “V-type” array microelectrode on a grating PDMS substrate.

First, the “V-type” array structure on the silicon wafer was fabricated by the MEMS technology, and the fabrication process included ultra-violet lithography and silicon etching. The “V-type” array structure on the master mold was then replicated into polycarbonate, which served as an intermediate, negative mold, using a conventional nanoimprint lithography technique. The negative mold was subsequently used in the PDMS molding process to produce PDMS “V-type” array structures with the same structures as the master mold. An Ag film was coated on the PDMS “V-type” array structure surface by the magnetron sputtering process to obtain PDMS “V-type” array microelectrodes. Finally, a PDMS prepolymer was prepared using a Sylgard184 curing agent with a weight ratio of a 20:1 and applied to the cavity at the middle of the two-layer PDMS “V-type” array microelectrode template to complete hot-press bonding, and a pressure sensor was realized.

The experimental results showed that the PDMS “V-type” array microelectrode has high stretchability of 65 per cent, temperature stability of 0.0248, humidity stability of 0.000204, bending stability and cycle stability. Capacitive pressure sensors with a “V-type” array microelectrode exhibit ideal initial capacitance (111.45 pF), good pressure sensitivity of 0.1143 MPa-1 (0-0.35 Mpa), fast response and relaxation times (<200 ms), high bending stability, high temperature/humidity stability and high cycle stability.

The PDMS “V-type” array structure microelectrode can be used to fabricate pressure sensors and is highly flexible, crack-free and durable.

This paper aims to present the capacitance characterization of tapered dielectrophoresis (DEP) microelectrodes as micro-electro-mechanical system sensor and actuator device. The application of DEP-on-a-chip (DOC) can be used to evaluate and correlate the capacitive sensing measurement at an actual position and end station of liquid suspended targeted particles by DEP force actuator manipulation.

The capability of both, sensing and manipulation was analysed based on capacitance changes corresponding to the particle positioning and stationing of the targeted particles at regions of interest. The mechanisms of DEP sensor and actuator, designed in DOC applications were energized by electric field of tapered DEP microelectrodes. The actual DEP forces behaviour has been also studied via quantitative analysis of capacitance measurement value and its correlation with qualitative analysis of positioning and stationing of targeted particles.

The significance of the present work is the ability of using tapered DEP microelectrodes in a closed mode system to simultaneously sense and vary the magnitude of manipulation.

The integration of DOC platform for contactless electrical-driven with selective detection and rapid manipulation can provide better efficiency in in situ selective biosensors or bio-detection and rapid bio-manipulation for DOC diagnostic and prognostic devices.

This review aims to focus on recent reported research work on the construction and function of different electrochemical DNA biosensors. It also describes different sensing materials, chemistries of immobilization probes, conditions of hybridization and principles of transducing and amplification strategies.

The human disease-related mutated genes or DNA sequence detection at low cost can be verified by the electrochemical-based biosensor. A range of different chemistries is used by the DNA-based electrochemical biosensors, out of which the interactions of nanoscale material with recognition layer and a solid electrode surface are most interesting. A diversity of advancements has been made in the field of electrochemical detection.

Some important aspects are also highlighted in this review, which can contribute in the creation of successful biosensing devices in the future.

This paper provides an updated review of construction and sensing technologies in the field of biosensing.

The purpose of this paper is to use a particle velocity measurement technique on a tapered microelectrode device via changes of an applied voltage, which is an enhancement of the electric field density in influencing the dipole moment particles. Polystyrene microbeads (PM) have used to determine the responses of the dielectrophoresis (DEP) voltage based on the particle velocity technique.

Analytical modelling was used to simulate the particles’ polarization and their velocity based on the Clausius–Mossotti Factor (CMF) equation. The electric field intensity and DEP forces were simulated through the COMSOL numerical study of the variation of applied voltages such as 5 V p-p, 7 V p-p and 10 V p-p. Experimentally, the particle velocity on a tapered DEP response was quantified via the particle travelling distance over a time interval through a high-speed camera adapted to a high-precision non-contact depth measuring microscope.

The result of the particle velocity was found to increase, and the applied voltage has enhanced the particle trajectory on the tapered microelectrode, which confirmed its dependency on the electric field intensity at the top and bottom edges of the electrode. A higher magnitude of particle levitation was recorded with the highest particle velocity of 11.19 ± 4.43 µm/s at 1 MHz on 10 V p-p, compared to the lowest particle velocity with 0.62 ± 0.11 µm/s at 10 kHz on 7 V p-p.

This research can be applied for high throughout sensitivity and selectivity of particle manipulation in isolating and concentrating biological fluid for biomedical implications.

The comprehensive manipulation method based on the changes of the electrical potential of the tapered electrode was able to quantify the magnitude of the particle trajectory in accordance with the strong electric field density.

The purpose of this paper is to provide a technical insight into recent developments in brain‐computer interface (BCI) technology and its applications.

Following an introduction to BCI, this paper considers the different means of detecting brain waves and then discusses a number of BCI development programmes and applications.

Invasive, partially invasive and non‐invasive BCI techniques are the topic of extensive study and aim to allow the control of external devices by human thought. Invasive BCIs are being applied to patients suffering paralysis and requiring cranial surgery and aim to restore movement or impart other functions such as the operation of robotic arms or communication. Wireless invasive microelectrode arrays are under development. Of the non‐invasive techniques, electroencephalography is attracting the greatest interest and is being applied to a range of military and healthcare uses.

This paper provides an introduction to BCI technology and a review of recent research and a number of key applications.