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This study/paper aims to the authors applied low “Si” ions dose over cp-Ti-2, and the potent dose level was optimized for adequate corrosion resistance and effective proliferation of stem cells.

The cp-Ti surface was modified by silicon (Si) ions beam at 0.5 MeV in a Pelletron accelerator. Three different ion doses were applied to the polished samples, and the surface was characterized by XRD and AFM analysis.

At moderate “Si” ion dose (6.54 × 10¹² ions-cm⁻²), the potential shifted to a noble value. The small “i_corr” (1.22 µA.cm⁻²) and relatively large charge transfer resistance (43.548 kΩ-cm²) in the ringer‘s lactate solution was confirmed through Potentiodynamic polarization and impedance spectroscopy analysis. Compared to cp-Ti and other doses, this dose level also provided the effective proliferation of mesenchymal stem cells.

The dosage levels used were different to previous work and provided the effective proliferation of mesenchymal stem cells.

The present paper is an attempt to explore the emerging stem cell innovation system in India. It is contended that the social capital in terms of linkages of various sorts can no longer be ignored to strengthen the innovation system and that the coevolution of technology and institutions is yet to emerge. It seems that given the nature of complex technologies involved, there is a greater need felt for R&D and training collaboration and hence linkages of various types are taking place. For shaping futures for a balanced growth of this sector, the institutions in India will have to be geared towards greater coordination, promotion of greater knowledge flows at national as well international levels. This paper also analyses the strengths and barriers in the development of rapidly growing stem cell research in India along with future challenges.

The purpose of this study is to show how selective laser sintering (SLS) manufacturing of bioresorbable scaffolds is used for applications in bone tissue engineering.

Polycaprolactone (PCL) scaffolds were computationally designed and then fabricated via SLS for applications in bone and cartilage repair.

Preliminary biocompatibility data were acquired using human mesenchymal stem cells (hMSCs) assuring a satisfactory scaffold colonization by hMSCs.

A promising procedure for producing porous scaffolds for the repair of skeletal defects, in tissue engineering applications, was developed.

The purpose of this paper is to focus on the advantages of a robotic time‐lapsed microscopic imaging system for tracking stem cells in in vitro biological assays which measure stem cell activities.

The unique aspects of the system include robotic movement of stem cell culture flasks which enables selection of a large number of regions of interest for data collection. Numerous locations of a cell culture flask can be explored and selected for time‐lapsed analysis. The system includes an environmentally controlled chamber to maintain experimental conditions including temperature, gas levels, and humidity, such that stem cells can be tracked by visible and epifluorescence imaging over extended periods of time.

This is an extremely unique system for both individual cell tracking and cell population tracking in real‐time with high‐throughput experimental capability. In comparison to a conventional manual cell culture and assay approach, this system provides stem cell biologists with the ability to quantify numerous and unique temporal changes in stem cell populations, this drastically reduces man‐hours, consumes fewer laboratory resources and provides standardization to biological assays.

Fundamental basic biology questions can be addressed using this approach.

Stem cells are often available only in small numbers – due both to their inherent low frequency in the post‐natal tissue as compared to somatic cells, and their slow growth rates. The unique capabilities of this robotic cell culture system allow for the study of cell populations which are few in number.

The robotic time‐lapsed imaging system is a novel approach to stem cell research.

Research with stem cells is a biomedical venture with great scientific impact, and whose development flows over into many other areas. This article aims to present a dual analysis of Spain's scientific output in this field during the period 1997‐2007.

The authors used bibliometric indicators of a basic nature as well as techniques for the visualization and analysis of networks of scientific information based on a study of KeyWords Plus.

The output is mainly concentrated in Cataluña and Madrid, and hospitals are the most productive centres (followed by health institutes), where the main authors are affiliated. Main categories are hematology, oncology and biophysics. The outstanding areas of study revolve around the therapeutic use of transplant of hematopoietic progenitors, the processes of generation, proliferation and differentiation of lines of cells, and the study of neurosciences.

This study provides an overview of Spanish research involving stem cells, detecting and representing the main areas of research. The article considers the potential of KeyWords Plus in combination with the proposed methodology as particularly useful for the analysis and delimitation of a scientific domain.

Marketing.

This case is suitable for MBA/MS courses for students of services marketing; courses such as sustainable development of business and integrated marketing communications.

Cordlife Limited entered the Indian market for cord blood banking in 2006 and by 2011 held third place in market share. However, the management of Cordlife had identified a major problem as a lack of awareness of the potential of cord blood banking among the Indian middle class, and the lack of a proper infrastructure for transportation of biological packages. Cordlife undertook several marketing initiatives to spread awareness. Marketing such a sophisticated service like cord blood banking called for heavy investments. The case provides an opportunity to closely examine various marketing activities in detail and understand how problems associated with intangible services can be managed. In addition to marketing of services the case highlights the existence of several gaps in designing a delivery in a service. The scope of the case can also be extended to the concept of service pricing and also integrated services marketing communications.

The case is designed for class discussions and in understanding the following concepts: the service gaps model; service pricing; and integrated service marketing communications.

Teaching notes are available. Consult your librarian for access.

Additive manufacturing (AM) or solid freeform fabrication (SFF) technique is extensively used to produce intrinsic 3D structures with high accuracy. Its significant contributions in the field of tissue engineering (TE) have significantly increased in the recent years. TE is used to regenerate or repair impaired tissues which are caused by trauma, disease and injury in human body. There are a number of novel materials such as polymers, ceramics and composites, which possess immense potential for production of scaffolds. However, the major challenge is in developing those bioactive and patient-specific scaffolds, which have a required controlled design like pore architecture with good interconnectivity, optimized porosity and microstructure. Such design not only supports cell proliferation but also promotes good adhesion and differentiation. However, the traditional techniques fail to fulfill all the required specific properties in tissue scaffold. The purpose of this study is to report the review on AM techniques for the fabrication of TE scaffolds.

The present review paper provides a detailed analysis of the widely used AM techniques to construct tissue scaffolds using stereolithography (SLA), selective laser sintering (SLS), fused deposition modeling (FDM), binder jetting (BJ) and advanced or hybrid additive manufacturing methods.

Subsequently, this study also focuses on understanding the concepts of TE scaffolds and their characteristics, working principle of scaffolds fabrication process. Besides this, mechanical properties, characteristics of microstructure, in vitro and in vivo analysis of the fabricated scaffolds have also been discussed in detail.

The review paper highlights the way forward in the area of additive manufacturing applications in TE field by following a systematic review methodology.

This study aims to investigate the effect of three-dimensional (3D)- bioplotted polycaprolactone (PCL) scaffold geometry on the biological and mechanical characteristics of human adipose-derived stem cell (hASC) seeded constructs.

Four 3D-bioplotted scaffold disc designs (Ø14.5 × 2 mm) with two levels of strand–pore feature sizes and two strand laydown patterns (0°/90° or 0°/120°/240°) were evaluated for hASC viability, proliferation and construct compressive stiffness after 14 days of in vitro cell culture.

Scaffolds with the highest porosity (smaller strand–pore size in 0°/120°/240°) yielded the highest hASC proliferation and viability. Further testing of this design in a 6-mm thick configuration showed that cells were able to penetrate and proliferate throughout the scaffold thickness. The design with the lowest porosity (larger strand–pore size in 0°/90°) had the highest compression modulus after 14 days of culture, but resulted in the lowest hASC viability. The strand laydown pattern by itself did not influence the compression modulus of scaffolds. The 14-day cell culture also did not cause significant changes in compressive properties in any of the four designs.

hASC hold great potential for musculoskeletal tissue engineering applications because of their relative ease of harvest, abundance and differentiation abilities. This study reports on the effects of 3D-bioplotted scaffold geometry on mechanical and biological characteristics of hASC-seeded PCL constructs. The results provide the basis for future studies which will use this optimal scaffold design to develop constructs for hASC-based osteochondral tissue engineering applications.

Bioprinting is a promising technology, which has gained a recent attention, for application in all aspects of human life and has specific advantages in different areas of medicines, especially in ophthalmology. The three-dimensional (3D) printing tools have been widely used in different applications, from surgical planning procedures to 3D models for certain highly delicate organs (such as: eye and heart). The purpose of this paper is to review the dedicated research efforts that so far have been made to highlight applications of 3D printing in the field of ophthalmology.

In this paper, the state-of-the-art review has been summarized for bioprinters, biomaterials and methodologies adopted to cure eye diseases. This paper starts with fundamental discussions and gradually leads toward the summary and future trends by covering almost all the research insights. For better understanding of the readers, various tables and figures have also been incorporated.

The usages of bioprinted surgical models have shown to be helpful in shortening the time of operation and decreasing the risk of donor, and hence, it could boost certain surgical effects. This demonstrates the wide use of bioprinting to design more precise biological research models for research in broader range of applications such as in generating blood vessels and cardiac tissue. Although bioprinting has not created a significant impact in ophthalmology, in recent times, these technologies could be helpful in treating several ocular disorders in the near future.

This review work emphasizes the understanding of 3D printing technologies, in the light of which these can be applied in ophthalmology to achieve successful treatment of eye diseases.

The 3 D bioprinting technology is used to prepare the tissue engineering scaffold with precise structures for the cell proliferation and differentiation.

According to the characteristics of the ideal tissue engineering scaffold, the microstructural design of the tissue engineering scaffold is carried out. The bioprinter is used to fabricate the tissue engineering scaffold with different structures and spacing sizes. Finally, the scaffold with good connectivity is achieved and used to cell PC12 culture.

The results show that the pore structure with the line spacing of 1 mm was the best for cell culture, and the survival rate of the inoculated cells PC12 is as high as 90%. The influence of the pore shape on the cell survival is not evidence.

This study shows that tissue engineering scaffolds prepared by 3 D bioprinting have graded structure for three-dimensional cell culture, which lays the foundation for the later detection of drug resistance.