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Mixed fermentation with Saccharomyces cerevisiae and non-Saccharomyces yeasts has become an oenlogical tool to improve wines’ organoleptic properties. However, the maximum utilization of this method is dependent upon understanding the influence of mixed cultures on the physiology of S.cerevisiae and non-Saccharomyces yeasts.

In this study, the supernatants from 48 h mixed-culture fermentation were added to the pure cultures of Issatchenkia orientalis and Saccharomyces, respectively. And the authors used RNA sequencing to determine the transcriptome change of I.orientalis and S.cerevisiae in a mixed culture.

The results showed that multiple genes associated with cell growth and death were differentially expressed. Genes related to biosynthesis of amino acids were enriched among those upregulated in the mixed-fermentation supernatant. Meanwhile, the differential expression level of genes encoding enzymes essential for formation of aroma compounds was found in the single and in the mixed fermentation. The high expression level of molecular chaperones Hsp70, Hsp90 and Hsp110 suggests that metabolites of mixed-culture fermentation may lead to aggregation of misfolded proteins. Moreover, upregulation of ethanol dehydrogenase I ADH1 in the mixed-culture fermentations was highlighted.

This is the first time that RNA-seq was used to analyze changes in the transcriptome of mixed cultures. According to the results the authors’ manuscript provided, an integrated view into the adaptive responses of S.cerevisiae and non-Saccharomyces yeasts to the mixed-culture fermentation is benefit for the potential application of S.cerevisiae and non-Saccharomyces yeasts in fruit wine brewing.

This paper considers the role of biosensors for monitoring the environment. A selection of existing products and competitive techniques such as biochemical assays and laboratory analysis which presently dominate the business are discussed. A number of research activities and themes are considered. Conclusion are drawn on the prospects for environmental biosensors.