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The purpose of this study is to compare the effect of olive or salmon oil on the hepatic storage and transport of fatty acids by very-low-density lipoproteins (VLDL).

In all, 24 male Wistar rats (80 ± 5 g) were fed a 0.5 per cent cholesterol-enriched diet with either 20 per cent casein (C) or chickpea (CP) proteins with 10 per cent olive (O) or salmon (S) oil for 28 days.

In VLDL-triacyglycerols fatty acids, oleic acid content was higher in CPS as compared to that in CS or CPO and lower in CS and CPO than that in CO; linoleic acid content was higher in all groups; arachidonic acid content was higher in CS and CPO as compared to that in CO. In the liver, TG fatty acids content was lower in CPO or CPS as compared to that in CO or CS; oleic and arachidonic acid contents were lower in CPS than that in CPO; linoleic acid content was lower in CS, CPS and CPO than that in CO, CPO and CO. In liver, phospholipid fatty acid, oleic and arachidonic acid contents were lower in CPS than that in CS; oleic, linoleic and arachidonic acid contents were lower in CPO compared to that in CO. In liver, cholesteryl esters fatty acids, oleic, linoleic and arachidonic acids contents were higher in CPS as compared to that in CS; oleic, linoleic and arachidonic acid contents were lower in CS as compared to that in CO; linoleic and arachidonic acid contents were lower in CPS than that in CPO.

A cholesterol-enriched diet containing casein or chickpea proteins combined with olive or salmon oil affects the hepatic storage and transport of polyunsaturated and monounsaturated fatty acids by VLDL.

The purpose of this paper is to evaluate the pathological, biochemical and redox state parameters of liver tissue in Wistar rats treated from birth to adulthood (119 days) with cafeteria diet.

During the lactation, 6 liters of Wistar rats (dam + 8 pups each) were fed one of two diets: control (CTRL; n = 3) or cafeteria (CAF; n = 3) diets and water ad libitum. After weaning, the males were placed in individual cages, receiving the same diet offered to their respective dams (CTRL or CAF; n = 18) until adulthood. The following parameters were evaluated: absolute and relative liver weight; blood, liver and feces biochemistry; liver histology; and redox state of the liver.

When assessing the relative and absolute organ weight, no significant differences were found between the groups. The Cafeteria group exhibited higher values of serum LDL-c (p = 0.008), VLDL-c (p = 0.03) and triglycerides (p = 0.01), as well as several micro and macrovacuoles of fat accumulation, higher hepatic lipid (p = 0.03) and cholesterol (p = 0.0001) levels regarding Control group. Cafeteria group showed greater expression of glutathione-s-transferase (p = 0.03) and superoxide dismutase (p = 0.005) enzymes compared to the control group. In the case of the markers of oxidative stress, there was no difference between the groups.

A simple and standardized cafeteria diet caused an accumulation of fatty acids in liver tissue, inducing a state of hepatic steatosis besides an increased expression of antioxidant enzymes.

The aim of the present study was to investigate the association of the empirical dietary index for insulin resistance (EDIR) and empirical lifestyle index for insulin resistance (ELIR) with the risk of nonalcoholic fatty liver disease (NAFLD) in Iranian adults.

In this case-control study, 120 cases of NAFLD and 240 controls aged ≥20 years were included. NAFLD was detected by a gastroenterologist using an ultrasonography test. The food frequency questionnaire was used to collect nutritional data and determine the score of EDIR in participants. ELIR was determined based on body mass index, physical activity and dietary pattern. The odds ratios (ORs) of NAFLD were reported across tertiles of EDIR and ELIR using a logistic regression test.

The mean±SD age and BMI of subjects were 41.8 ± 7.5 years and 27.4 ± 2.2 kg/m², respectively. In the age and sex-adjusted model, the odds of NAFLD were increased across tertiles of ELIR (OR = 3.00; 95% CI: 1.63–5.55, P_trend = 0.001). Also, based on the fully adjusted model, the odds of NAFLD were increased according to tertiles of ELIR (OR = 2.66; 95% CI: 1.38–5.10, P_trend = 0.006). However, no significant association was found between the higher score of EDIR and odds of NAFLD based on the age and sex-adjusted model (OR = 1.18; 95% CI: 0.68–2.05, P_trend = 0.52) and the multivariable-adjusted model (OR = 0.91; 95% CI: 0.48–1.70, P_trend = 0.87).

To the best of the authors’ knowledge, this was the first study to examine the role of the insulinemic potential of diet and lifestyle in predicting NAFLD risk. Our findings suggested that a lifestyle with a higher score of ELIR was positively associated with NAFLD risk. However, a diet with a higher score of EDIR was not related to the odds of NAFLD.

The debate on the metabolic effects of high fructose corn syrup (HFCS) continues. The deterioration of endoplasmic reticulum (ER) homeostasis is called ER stress. Glucose-regulated protein-78 (GRP-78) and X-box binding protein-1 (XBP-1) are key markers of ER stress and the therapeutic targets of diseases. Sterol regulatory element binding protein-1c (SREBP-1c) is the most important transcription factor that regulates the expression of enzymes for fatty acid synthesis. The purpose of this paper is to research the effects of L-carnitine and trans-chalcone on ER stress and oxidative stress parameters, and to explore the therapeutic potential of L-carnitine and trans-chalcone molecules.

Forty male wistar albino rats randomly selected were divided into five groups. All groups are fed with standard chow (ad libitum). While Group I was fed with drinking water, Group II, III, IV and V were fed with water containing 15% HFCS. L-carnitine was given to Group IV and trans-chalcone to Group V, and both were dissolved with DMSO and given intraperitoneally. Group III was not given anything additional.

While the amount of water consumption of HFCS-fed rats has increased, the amount of feed consumption has decreased. The weights of rats in Group II and Group III have increased significantly compared to Group I (p = 0.001, p = 0.001 respectively). In Group III, GRP78, XBP-1; malondialdehyde level (p < 0.001, p = 0.001, p = 0.041); total cholesterol, triglyceride, LDL levels (p = 0.001, p < 0.001, p = 0.009, p = 0.001, respectively) have increased significantly.

To the best of the authors’ knowledge, this study is the first report to show that excessive HFCS consumption causes oxidative stress and ER stress. The antioxidant and antiobesity properties of trans chalcone have been demonstrated. Extensive experimental and clinical studies should be conducted.

First January 1973 will not only mark the beginning of a New Year but a year which history will mark as a truly momentous one, for this is the year that Britain, after centuries of absence, re‐enters the framework of Europe as one of the Member‐States of the enlarged European Community. This in itself must make for change on both sides; Britain is so different in outlook from the others, something they too realize and see as an acquisition of strength. There have been other and more limited forms of Continental union, mainly of sovereignty and royal descent. Large regions of France were for centuries under the English Crown and long after they were finally lost, the fleur de lis stayed on the royal coat of arms, until the Treaty of Amiens 1802, when Britain retired behind her sea curtain. The other Continental union was, of course, with Hanover; from here the Germanized descendants of the Stuarts on the female line returned to the throne of their ancestors. This union lasted until 1832 when rules of descent prevented a woman from reigning in Hanover. It is interesting to speculate how different history might have been if only the British Crown and the profits of Tudor and Stuart rule had been maintained in one part of central Europe. However, Britain disentangled herself and built up overwhelming sea power against a largely hostile Europe, of which it was never conceived she could ever be a part, but the wheel of chance turns half‐circle and now, this New Year, she enters into and is bound to a European Community by the Treaty of Rome with ties far stronger, the product of new politico‐economic structures evolved from necessity; in a union which cannot fail to change the whole course of history, especially for this country.

Adipose tissue accumulation by trapping vitamin D and reducing its level may cause serious side effects. The purpose of this study is to determine the effects of vitamin D supplementation on dehydroepiandrosterone (DHEA), paraoxonase 1 (PON 1), insulin, free fatty acid (FFA), apolipoprotein-AI (Apo-AI) and apolipoprotein B (Apo-B) concentration in obese and overweight participants under low-calorie diet (LCD) program.

Healthy overweight and obese individuals (n = 70) with vitamin D deficiency were randomly assigned into 2 groups to receive either vitamin D supplements (an oral 2,000 IU vitamin D supplement) or placebo for 8 weeks.

All the participants were given an LCD program during the intervention. Vitamin D supplementation led to a significant increase in the levels of 25(OH)D (vitamin D vs placebo groups: 36.6 ± 9.8 vs 19.9 ± 3.5 ng/mL, p < 0.001), PON 1 levels (vitamin D vs placebo groups: 80 ± 25 vs 58 ± 23.2 ng/mL, p = 0.001), DHEA concentration (vitamin D vs placebo groups: 2.3 ± 0.7 vs 1.5 ± 0.6 ng/mL, p < 0.001) and Apo-AI levels (vitamin D vs placebo groups 3.7 ± 0.5 vs 3 ± 0.5 mg/dL, p < 0.001). Besides, intake of vitamin D supplements led to a significant decrease in FFA (vitamin D vs placebo groups: 3.1 ± 0.75 vs 3.5 ± 0.5 ng/mL, p = 0.001). After adjusting the analyses based on baseline levels, age and baseline body mass index measures, significant changes were observed in the insulin levels (0.03 ± 0.06 vs −1.7 ± 0.6 µIU/ml, p = 0.04). But the authors did not find any significant difference in the concentration of Apo-B between groups (vitamin D vs placebo groups: 71.5 ± 35.5 vs 66.6 ± 28.5 mg/dL, p = 0.05).

Overall vitamin D supplementation for eight weeks among vitamin D-deficient obese and overweight participants had beneficial effects on serum DHEA PON 1 FFA insulin and Apo- AI while it did not affect the Apo-B concentration.

This paper aims to investigate the effect of Citrus maxima juice on fasting blood glucose, lipid profile, liver enzyme and body weight of alloxan-induced diabetic adult wistar rats.

The fruits were thoroughly washed with potable water. They were peeled; the epicarp and seeds were removed. Fruit juice was extracted from the pulp. Animal experiment was carried out using 21 adult male wistar rats weighing about 110-130 g. The rats were categorized into three groups (A-C), with each group consisting of seven rats of similar body weights. Diabetes was induced using alloxan. Rats in Groups A and B were administered 300 mg and 600 mg of C. maxima fruit juice/kg body weight/day, respectively, for 14 days, while Group C rats acted as control and received placebo. Biochemical analysis was performed using standard procedures. Data were analyzed using SPSS, version 21.

Blood glucose of rats in the treated groups decreased significantly (p < 0.05) from 454.7 and 569.7 mg/dl to 149.3 and 297.3 mg/dl, respectively, while the control increased from 257.0 to 46.57 mg/dl. Total cholesterol and triglyceride levels of Group B rats reduced significantly (p < 0.05), while the high-density lipoprotein cholesterol level increased (p < 0.05). Body weight of rats in the treated groups increased significantly (p < 0.05) by 30.1 and 20.2 per cent in Groups A and B, respectively, compared to the control group, which reduced by 0.21 per cent. There was no significant effect on markers of liver damage.

The rate at which diabetes and other non-communicable diseases increase recently calls for affordable and sustainable management.

Diabetes is one of the major health and development challenges of the 21st century, its complications are disabling and life threatening.

The hypoglycemic and hypocholesterolemic properties demonstrated by C. maxima fruit juice suggest its potential contribution in reducing/stabilizing blood glucose level and managing complications of diabetes.

The purpose of this study is to investigate the effect of olive cake (OC) on oxidant/antioxidant biomarkers, lipase activity and on the histological analysis of epididymal fat, in high-fat diet (HFD)-induced obese rats.

Male obese rats were divided into two groups and were fed an HFD supplemented (HFD-OC) or not (HFD) with OC for 28 days. A control group was fed a standard diet for the same experimental period.

HFD significantly increased body weight, which was reduced by OC in the HFD-OC compared to HFD (p = 0.038). Lipase activity was higher (52%; p = 0.009) in the HFD group than the control group. Administration of OC to the obese rats decreased significantly this activity (38%; p = 0.025) compared to the HFD group. Serum thiobarbituric acid-reactive substances, lipid hydroperoxide and advanced oxidation protein products levels were significantly increased in the HFD group than the control group (p = 0.032, p = 0.023 and p = 0.017, respectively). These levels were significantly reduced in HFD-OC compared to the HFD group (p = 0.030, p = 0.021 and p = 0.010, respectively). Superoxide dismutase, glutathione peroxidase and catalase activities were decreased (53%; p = 0.04), (61%; p = 0.03) and (32%; p = 0.002), in the HFD group than the control group. OC restored these activities (46%; p = 0.01), (58%; p = 0.003) and (30%; p = 0.0003) in the HFD-OC rats than the HFD rats. Consumption of the HFD resulted in adipocyte hypertrophy. Indeed, epididymal adipocyte size was significantly larger in the HFD group than the control group (p = 0.0001), whereas it was reduced in the HFD-OC compared to the HFD group (p = 0.012).

OC possesses an anti-obesity effect. This effect might be mediated by lipase inhibition, reduced oxidative stress and increased antioxidant activities. In addition, the reduction of fat accumulation in adipose tissue by OC consumption is reflected by reducing adipocyte size.

In this second part of the review the roles that copper and dietary carbohydrate play in the cardiovascular system, pregnancy and lactation, haemopoiesis, and in male and female animals, are summarised. The mechanisms that may contribute to the exacerbation of copper deficiency when simple sugars are consumed are discussed.