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The aim of this paper is to present risk analysis procedures which have been successfully applied by Czech small and medium enterprises (SMEs). The methodology, which is based on the modification and combination of two standard methods, aims to accelerate (and make more affordable) the risk analysis process, as compared to other risk analysis methods used for public organizations and major corporations in the Czech Republic.

The paper presents in detail the individual steps the authors used in risk analysis of SMEs in the Czech Republic. The method is based on the facilitated risk analysis process (FRAP) methodology and the BITS recommendation. Modifications of both methodologies are described in detail.

To perform risk analysis in the SME sector in the Czech Republic, it is necessary to have a broad portfolio of instruments. Besides using the CRAMM methodology, the authors have created a new method based on combining the BITS and FRAP methods. The advantage of this method is its ability to accelerate the risk analysis, especially the identification and asset evaluation phases. Another advantage is that the method produces simple spreadsheet tables, providing the consumer with a tool that is easily editable and may be used for follow‐up procedures.

The risk analysis method produces benefits for SMEs by speeding up the risk analysis and lowering its cost. Another benefit is that the method is open‐source and can potentially be further modified.

The paper presents in detail an approach to risk analysis based on the modification of the FRAP methodology and the BITS recommendation.

Literature has consistently reported that coloured plants are rich in dietary bioactive compounds. Therefore, this study aims to compare the total phenolic content and antioxidant activities in selected coloured plants (blue butterfly pea flower, roselle calyx, yellow bell pepper and purple sweet potato).

Total Phenolic Content (TPC) was determined using Folin–Ciocalteu assay, while antioxidant activities were evaluated using 2,2-dophenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging, Ferric Reducing Antioxidant Power (FRAP) and Trolox Equivalent Antioxidant Capacity (TEAC) assays.

TPC was reported from 273.15 ± 19.57 µg GAE/g DW (blue butterfly pea flower extract) to 363.10 ± 7.94 µg GAE/g DW (roselle calyx extract). Antioxidant activities as determined by DPPH assay ranged from 17.26 ± 0.06% (purple sweet potato extract) to 83.38 ± 1.04% (yellow bell pepper extract); while for FRAP assay was 4.92 ± 0.18 mg Fe (II)/g DW (purple sweet potato extract) to 128.33 ± 11.59 mg Fe (II)/g DW (roselle calyx extract). On the other hand, TEAC values were in the range of 15.26 ± 2.83 µg Trolox/g DW (roselle calyx extract) to 364.27 ± 7.14 µg Trolox/g DW (blue butterfly pea flower extract). A significant moderate positive correlation was observed between TPC and DPPH (r = 0.562) as well as TPC and FRAP (r = 0.686).

This study was the first to compare the total phenolic content and antioxidant activities in coloured plants. Findings derived from this study can be extended to the formulation of natural food colourants and nutraceuticals.

This paper aims to study the effect of cooking and food additives, such as lemon juice and vinegar, on phenols and flavonoids contents and antioxidant activity of purslane.

The Folin–Ciocalteu method was used to determine total phenols content (TP), while total flavonoid content (TF) was determined by the aluminum chloride method. Two methods were used for determination of antioxidant activity: DPPH (1, 1-diphenyl-2-picrylhydrazyl) assay to determine radical scavenging activity, and ferric-reducing antioxidant power (FRAP) to measure the reducing power.

According to the results, leafs had higher values of TP, TF and antioxidant activity than aerial parts. Both lemon juice and vinegar retracted antioxidant properties of leafs. TP and TF of leaves showed deterioration after treatment with lemon by 58% and 21.8%, respectively, and FRAP and radical scavenging activity decreased by 75.8% and 74.5%, respectively (p < 0.001). Also, TP, TF, FRAP and DPPH radical scavenging activity decreased in leaves by 82.2%, 30.5%, 87.8% and 90.9%, respectively, after treatment of leaves with vinegar. TF increased after cooking in studied parts, where no significant statistical difference was observed in TP and antioxidant activity (DPPH assay and FRAP) of cooked aerial parts. Adding lemon juice after cooking increased antioxidant properties of purslane (p < 0.001).

Purslane has antioxidant activity because it is rich in polyphenols and flavonoids. Effects of food additives and cooking were studied using different measurements. According to the authors’ knowledge, this is the first work that studied the effect of food additives on antioxidant properties of purslane.

The purpose of this study was to evaluate in vivo the bioavailability of the Ilex paraguariensis polyphenols due to total polyphenols and changes in antioxidant capacity (AOC) in human plasma after an acute intake of 300 ml of an infusion of yerba maté (YM) for 120 minutes. Also, we evaluated the variation of plasma protein or plasma uric acid after the acute intake of YM infusion.

Seventeen healthy young volunteers participated in the determining plasma of total polyphenols concentration (TPC, Folin-Ciocalteu method), plasma AOC for ferric reducing antioxidant power (FRAP) and 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid diammonium salt [ABTS] methods), plasmatic uric acid and plasma total protein over the 120 minutes test.

It was found that the bioavailability of YM polyphenols during 120 minutes was 49.3 ± 11.9 per cent, the TPC was increased to 6.0 ± 1.5 per cent, the plasma AOC was increased by FRAP 8.3 ± 3.3 per cent and by ABTS 6.0 ± 2.0 per cent and no significant variation of plasma protein or plasma uric acid was found.

Maté polyphenols has a thrifty effect on the natural antioxidant defenses of the body, which are beneficial to human health.

There was no information on the bioavailability of polyphenols in YM infusions prepared in its traditional form as “hot mate”.

The purpose of this paper is to study the structure‐activity correlation of four medicinal plants – Ocimum basilicum L., Piper betle L., Grewia asiatica L., Lantana camara L. in crude methanolic extract. These plants have been used in Ayurvedic preparation as alternative medicine. The contents of phenolic compounds (flavonoids, anthocyanin) and antioxidant capacities were evaluated.

Folin‐Ciocalteu method was used for the analysis of the phenolic compounds. Total flavonoids were also evaluated. DPPH, ABTS+, FRAP and H₂O₂ assays were used to evaluate antioxidant activity. Most of the assays were determined spectrometrically.

O. basilicum L. had the highest of total phenolics content (305.11 mg GAE/g), highest content of anthocyanins (15.427 mg/Kg) and total flavonoids (9.692 QE mg/g). The DPPH, ABTS+, FRAP and H₂O₂ assay indicated that these plants possessed considerable antioxidant activities.

The results also showed that O. basilicum and L. camara from red colored cultivars possessed high contents of phenolic compounds. FRAP assay showed AOA in the following sequence – Lantana camara>Ocimum basilicum>Piper betle>Grewia asiatica. Since L. camara showed highest antioxidant capacity thus it can be a potential resource for commercial antioxidant.

The objective of this study was to evaluate the technical-economic process efficiency of obtaining simultaneous lipo-soluble (LSF) and water-soluble (WSF) fractions from annatto seeds.

The batches of annatto seeds were submitted to the refrigerated solid-liquid extraction process in four stages: pre-extraction, aqueous extraction, separation by decantation and filtration. After that, LSF and WSF from annatto seeds were obtained. The process efficiency and the quality of LSF and WSF were analyzed in terms of average yield and bioactive compounds (bixin, norbixin, phenolics and flavonoids) and their antioxidant and antimicrobial activities. Furthermore, they were economically evaluated in terms of costs of manufacturing and profitability parameters.

The process was efficient in terms of overall average yield (LSF = 8.68% and WSF = 2.76%) (w/w) and in terms of quality, mainly with higher average yields of bixin (82.34% in LSF) and norbixin (29.59% in WSF) (w/w). The concentration of bioactive compounds in the fractions promoted an increase in inhibiting free radicals (DPPH* and ABTS*+) and in the ferric-reducing power (FRAP). LSF showed a minimum inhibitory concentration of 0.06 mg mL-1 for S. aureus and 0.13 mg mL-1 for S. Typhimurium and S. Enteritidis. The lowest manufacturing costs were obtained for the LSF due to its higher extraction yield compared to the WSF. Plants on an industrial scale of 100 and 1000 L were considered economically viable, with a return on investment of 5 and 2 years.

Thus, fractions (WSF and LSF) can be applied as natural additives, as sources of bioactive compounds for nutraceutical and/or pharmaceutical, and in the development of other innovative processes. These results have practical applicability for pharmaceutical and food industry.

1. Green processing of annatto seeds obtains fractions rich in antioxidant compounds.

2. Efficiently presents a high yield of bixin and other bioactive compounds.

3. Effective in concentrating compounds that inhibit microbial growth.

4. Fractions are more accessible sources of bioactive compounds for isolation.

5. Cost of manufacturing (COM) and profitability are studied.

Green processing of annatto seeds obtains fractions rich in antioxidant compounds.

Efficiently presents a high yield of bixin and other bioactive compounds.

Effective in concentrating compounds that inhibit microbial growth.

Fractions are more accessible sources of bioactive compounds for isolation.

Cost of manufacturing (COM) and profitability are studied.

Chili pepper pods are a worldwide used and cultivated spice with a high economic and cultural importance. Bolivia is a center of origin and diversification of important crops including wild pepper varieties, but little is known about the chemical composition, antioxidant activity and the Scoville and bioactive compounds of these chili peppers. The purpose of this study was to contribute with new data about the chemical composition and the Scoville scale of Bolivian Chili peppers.

A total of 26 samples of Bolivian Chili peppers were extracted using distilled water and methanol. Total antioxidant capacity was determined by the ferric-reducing antioxidant power (FRAP) and by the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) methods. The total phenolic content was determined by Folin and Ciocalteu. Carotene, protein and ashes were determined by Bolivian analytical norms. Capsaicin was determined by high-performance liquid chromatography. All determinations were expressed as mean values± standard deviation of six replicates measured over three days of one extract. All values were less than 5% of coefficient of variation. Principal component analysis was applied to reveal patterns in the data. PC1 and PC2 together explained 78% of the total variance.

The protein content (3.8–6.3 g/100 g of dw) and the antioxidant activity (88.2 to 374 by FRAP and 87.0 to 172 evaluated by ABTS) studied in these species revealed that the values were above the average reported in the literature. The amount of pungency or capsaicin content expressed as Scoville Heat Units were reported moderately highly and very highly pungent (5,696–148,800 g dw) in several of the Bolivian peppers which demonstrates a good potential for capsaicin extraction and different applications for industry and as food ingredients.

This manuscript presents an important and novel contribution to the knowledge of the chili peppers in the region.

The paper aims to investigate the iron, zinc, carotenoid, vitamin E, anthocyanin, and phenolic contents, antioxidant activity and the effect of the boiling of rice on such parameters.

Iron and zinc content were investigated using atomic absorption spectrometry. Meanwhile, simultaneous extraction and detection methods using high performance liquid chromatography were applied for carotenoids, tocopherol, and tocotrienol analyses. For anthocyanin and phenolic determination, pH differential method and Folin Ciocalteu method were used, respectively. Antioxidant activity of samples was examined using ferric reducing antioxidant power (FRAP) and superoxide radical scavenging methods.

The n790 cultivar has a higher iron, zinc γ tocopherol (1,869.64 mg/kg), α tocopherol (1,883.97 mg/kg), anthocyanin (205.54 mg cyanidin 3 glucoside equivalent/100 g dry matter), and phenolic (728.9 mg catechin equivalent/100 g dry matter) contents. Jowo cultivar has higher lutein (1,314.21 mg/kg), α carotene (823.29 mg/kg), and β carotene (71.73 mg/kg) contents. Meanwhile, γ tocotrienol is the most abundant tocotrienol found in all samples. N790 cultivar had the highest antioxidative potential in FRAP, and superoxide radical methods. Boiling did not significantly affect the zinc content, however, different response was found in carotenoid, tocopherol, and tocotrienol content. Moreover, boiling decreased the antioxidant activity of extract.

Black- and red-colored rice could substitute white rice in Indonesian diet to achieve better nutrient intake.

Providing information on nutrient and bioactive compound of Indonesian underutilized colored rice can help to change the dietary habit and to alleviate nutrient deficiency in Indonesia.

Salak (Salacca zalacca) is a species of palm tree native to Malaysia and Indonesia. The fruit is also known as “snake fruit” due to its reddish‐brown scaly skin. Four different varieties of the fruits (i.e. SS1, SS2, SS3 and SS4) have been established by Sabah State Agriculture Department, Malaysia. The purpose of this paper is to investigate and compare the antioxidant properties and phytochemicals content in the edible portion of the fruits.

Four different varieties of the fruits were collected and analysed for the antioxidant properties (2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) assay), total phenolic and total flavonoid contents using spectrophotometry analysis. Ascorbic acid was determined using titration method.

The results showed the total phenolic and flavonoid contents of the samples were in the range of 12.6‐15.0 mg gallic acid equivalent/g and 4.9‐7.1 mg catechin equivalent/g of dry sample, respectively. The antioxidant activities of the extracts (using DPPH assay) were highly correlated with total phenolic and moderately correlated with flavonoid content. The reducing capabilities of the extracts using FRAP assay were moderately correlated with all phytochemicals tested. The results suggested that the phytochemicals and antioxidant activity of salak is mildly affected by variety. The high phytochemicals and antioxidant properties of S. zalacca indicated that the fruit possessed potential health benefits properties.

Salak fruit is now being developed into fruit juice, pickle and other food products. The commercialization of the fruit may be enhanced if more knowledge on its potential health benefits is studied and discovered. The economic and nutraceutical values might increase and will contribute greatly to the local people.

This study aims to evaluate the effect of grape seed extract on the thermal oxidation of soybean oil.

Four treatments were used: soybean oil (SO), butylhydroxytoluene (BHT), antioxidant at concentration of 100 mg/kg in soybean oil (BHTSO), grape seed extract at a concentration of 100 mg/kg in soybean oil (Extract), and mixture at a concentration of 50 mg/kg of grape seed extract (Mixture) and 50 mg/kg of BHT in soybean oil subjected to 180°C for 20 h. The total phenolic compounds were quantified by spectrophotometry using the Folin-Ciocalteu reagent. The antioxidant activity was evaluated by the ABTS^•+, DPPH^• and FRAP acid systems. The fatty acid composition and phytosterols were determined by gas chromatography (GC) and tocopherols were determined by high-performance liquid chromatography.

Among the treatments studied, the Extract and the Mixture presented lower losses of total phenolic compounds, DPPH^• and FRAP, while BHTSO had an opposite effect. After 10 h of heating, the Mixture and the Extract were more effective in reducing the formation decomposition products, measured by the amount of total polar compounds. The Mixture resulted in higher retention of essential fatty acids at the end of heating. The Extract and Mixture showed higher retention rates of total tocopherols and the Mixture resulted in 338.71 mg/100 g (P ≤ 0.05) of total phytosterol after 20 h of heating.

Oils undergoing the heating process suffer major changes, requiring the addition of antioxidant compounds. As a result of the questioning on the use of chemical additives, research on natural antioxidants, as substitute to the synthetic ones, is increasing. Among the natural compounds with high antioxidant potential, the grape wastes such as its seeds are highlighted. Studies using grape seed extract are scarce.