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The primary objective was to determine the prevalence of indicator microorganisms [Staphylococcus aureus, non-S. aureus staphylococci (NSAS), coliforms and aerobic bacteria] for contamination of chicken carcasses, carcass drip and rinse water from the informal chicken market in Gauteng, South Africa.

Chicken swabs, chicken drips and rinse waters were collected from 151 chickens from 47 random outlets. Pre-tested questionnaires were administered to capture the risk factors for bacterial contamination. Standard microbiological procedures were conducted for isolation and enumeration of target bacteria.

NSAS (64% and 41%) and S. aureus (12% and 31%) were prevalent on carcasses and in carcass drip respectively. Coliforms (62%) and aerobic bacteria (85%) were detected in rinse water. Significant risk factors for contamination of carcasses with NSAS, S. aureus and coliform organisms were: evisceration of chickens on the same location used for sale, cleaning of display counter with dirty clothes/wipes, holding of differently sourced chickens in the same cage prior to slaughter, not cleaning the display table/counter and hands at all, washing knives in rinse water, high turnover of daily slaughter and length of time to display chickens.

The limitations of this research were the limited geographical coverage and small sample size.

The isolation of these indicator microorganisms suggests the potential presence of other chicken-borne pathogens not tested for in the study.

The findings serve to inform policy on public health and street-vended food and can guide control on good sanitary practices.

This is the first comprehensive report on ready to eat chickens from the informal markets in Gauteng, South Africa.

The aim of the paper is to evaluate the microbiological and physico‐chemical characteristics of fermented pounded yam, a unique and popular food of the Igbominas of Nigeria.

Fresh pounded yam was prepared in the laboratory by a modification of the traditional method, and then fermented for varying periods of time (0‐96hrs), also essentially by the traditional method. Analysis was carried out on the products following documented and established procedures.

Data obtained indicated noticeable increases in the populations of all the groups of microorganisms investigated with increases in fermentation time, except coliforms which declined in population after 24 hours. Heating of the samples after each fermentation interval resulted in significant reductions in microbial populations such that coliforms could not even be detected after 24 hours of fermentation. Lactic acid production, an indication of flavour development, and pH drop were highest at 48 hours of fermentation. The samples also became softer with increases in fermentation period. The heated samples were considered safe microbiologically, since they contained neither coliforms nor faecal coliforms.

The consumption of fermented pounded yam is encouraged since it is safe microbiologically and develops a desired flavour, while further research should be carried out on the nutritional benefits.

The findings here suggested that adequate heating after fermentation is necessary to eliminate any potential microbial contamination, and for full flavour development.

The results of this research contribute to the knowledge of fermented foods, especially those that are indigenous to Nigeria and West Africa.

This paper aims to evaluate the microbial quality of some traditional cheese samples (sheep, cow and koopeh cheeses) consumed in northwest of Iran, and to detect Shiga-like-toxin-producing Escherichia coli (STEC) and methicillin-resistant Staphylococcus aureus (MRSA) in cheese samples by polymerase chain reaction (PCR) method.

Almost half of the project was based on counting the population of Staphylococcus aureus, total coliforms, Escherichia coli, and total aerobic mesophilic bacteria, also the other section was related to the isolation and the detection of the STEC and MRSA in cheese samples. The findings were compared with standard maximum and threshold values.

The results revealed that 36.99, 30.14 and 100% of cheeses exceeded the standard threshold value of E. coli (102), total coliforms (104) and S. aureus (102). However, total coliforms, in any of the cheese samples examined, did not reach the maximum value and only 24.66% of samples exceeded the maximum value of E. coli. Also, no significant difference (p > 0.05) in counts of each bacterial group examined in sheep, cow and koopeh cheeses was observed. The colony PCR method demonstrated the existence of 19 MRSA and 2 STEC isolates.

This research showed a general overview of the bacterial quality of cheeses in northwest of Iran.

Samples of sandwiches were taken from all petrol filling stations in the Bolton area selling such foodstuffs. These were bacteriologically examined and, when the results were obtained, all the petrol stations were visited and advice was given where improvements could be made. Resampling was carried out and the results were compared with the initial samples. The second set of results indicated a significant improvement in microbial flora. Further recommendations were made where necessary and it was possible to produce a Code of Practice from the results obtained.

The Public Health (Meat) Regulations, 1924 have at last been replaced. At the time of their making, they were hailed as a great advance towards an adequate meat inspection service; that they have lasted for almost forty years is not an indication, however, of their success in meeting the needs of the situation. They were too much of a compromise with vested interests; the great obstacle was the private slaughterhouse and complete freedom of action by the butcher. Notices and hours of slaughter and removal of carcases were all designed to help the butcher and not the inspector. In districts with many private slaughterhouses, widely scattered, they made the work of an inspector honestly trying to inspect all animals slaughtered very hard indeed. These difficulties made certain that inspection at the time of slaughter or immediately after of all animals slaughtered for food could only be practicable in the larger centres.

The purpose of this paper is to examine the efficacy of microbial elimination using different sanitizers in raw vegetables (cherry tomatoes, spring onions, Chinese chives, and chicory) and to analyze the efficacy of Escherichia coli O157:H7 reduction by type of sanitizer and vegetable.

To assess the sanitizing effects of microbial elimination by variety of vegetable, the samples were grouped into four different washing methods: control (no sanitizer), 100 ppm chlorine, 100 ppm electrolyzed water, and 200 ppm electrolyzed water after prewashing. Subsequently, quantitative microbiological experiments were conducted to assess aerobic mesophilic plate count (APC), coliform, E. coli, and Bacillus cereus, and sensory changes of the sanitized vegetables were tested. Thereafter, measurement of the sanitizing effects on bacterial reduction after inoculation with E. coli O157:H7 was conducted.

The microbial levels of four types of vegetables ranged from 3.37 to 5.24 log CFU/g for APC, 2.41 to 5.57 log CFU/g for E. coli, 0.25 to 5.40 log CFU/g for coliform, and 0.83 to 5.44 log CFU/g for B. cereus. After three types of sanitizing treatments, microbial reduction effects showed 0.94-1.84 log CFU/g for APC, 0.56-1.00 log CFU/g for E. coli, 0.18-1.26 log CFU/g for coliform, and 0.56-1.23 log CFU/g for B. cereus (p<0.05). In sensory evaluation, there were no significant differences in taste and flavor between with and without sanitizing treatments. Regarding bacterial reduction after inoculation with E. coli O157:H7, the microbial reduction on vegetables was shown to be in the range of 0.27-1.57 log CFU/g with 100 ppm sodium hypochlorite, 0.66-3.07 log CFU/g with 100 ppm electrolyzed water, and 0.79-2.55 log CFU/g with 200 ppm electrolyzed water. Chicory, cherry tomato, and spring onion showed significant reduction levels of E. coli O157:H7 after sanitation (p<0.05).

This study revealed that different sanitization methods are required for different types of vegetables. Electrolyzed water treatment (100 ppm) is a more effective and safe method of washing raw vegetables. Given that the main purpose of sanitizing fresh-cut produce is to maximally reduce microorganism levels, different methods of sanitizing fresh-cut produce with an adequate washing method should be used according to vegetable type.

The purpose of this paper is to examine the environmental and health impacts of abattoir wastes due to unhygienic disposal methods and inadequate slaughtering facilities as it affects some physicochemical and bacteriological parameters of Woji Creek and its environs.

The study involved the choice of five sampling stations for two different seasons: wet and dry. Standard methods were adopted for field and laboratory studies.

Water samples from station 2 (discharge point station) showed higher concentrations of turbidity, phosphate, biochemical oxygen demand, faecal coliform count for both dry and wet seasons, while dissolved oxygen values were lower at this station than in the other study stations. Water samples were slightly acidic (6.49‐6.70) for the five stations sampled during the study period. At the upstream station, the water had lower levels of turbidity, temperature, phosphate, nitrate, biochemical oxygen demand, faecal coliform count, but the values increased downstream after the discharge point station showing the impact of the abattoir effluents. The results further revealed seasonal influence on the parameters with higher values recorded during the dry season for conductivity, salinity, turbidity, phosphate, dissolved oxygen, biochemical oxygen demand, faecal coliform counts, temperature, total dissolved solids. This is because during the wet season the increased volume of water apparently diluted the concentration of abattoir wastes.

The work was carried out in order to establish the existing levels of the measured parameters and assess the pollution profile of the Creek as a result of abattoir operations.

This is the first time a holistic research has been conducted on Woji Creek. The paper has provided data and knowledge on which further research can be carried out to monitor changes in water quality caused by the activities of the abattoir operators.

In order to improve the safety of seafood in the domestic seafood distribution chains (DSDCs) in Vietnam, a better understanding of current hygiene and practices compliance with government regulations is needed. The paper aims to discuss this issue.

Infrastructure conditions and documents related to hygiene procedures of 50 trading places were evaluated using checklists. The hygiene handling practices of 135 fish distributors were also observed by using notational analysis methods. This notational analysis method focused on actions related to microbiological contamination in terms of hand washing or glove changing and the cleaning and sanitising of tools and equipment. Additionally, microbiological quality of 135 samples of raw finfish at points along distribution chains was compared with national standards.

The results indicated that all trading places could be classified as non-compliant or seriously non-compliant with the regulations. The practices of fish distributors were also assessed to be at high risk for contamination of raw fish. The findings showed that approximately 42 and 39 per cent of samples from fishing ports and fish markets, respectively were classified as unacceptable according to the microbiological standards of Vietnam.

The present study is limited to research only from fish landing at fishing ports to the distribution to consumers at retail markets.

Recommendations for improving food safety in the DSDCs in Vietnam have been developed from the findings of this study and are provided. However, due to similarities with fish distribution chains in other countries, these recommendations may have broader application.

The purpose of this paper is to investigate the effectiveness of NaOCl and KMnO₄ at two levels in reducing the microbial load of coriander leaves.

Fresh coriander leaves procured from various markets were intensively sampled and administered to wash treatments with tap water and sanitizers at two levels. Hi media pre plated plates were used for determination of TPC (Total Plate Count), Staphylococcus aureus, Listeria, Salmonella, Shigella and coliforms.

Unwashed coriander leaves showed TPC beyond the acceptable limits of 105 CFU/g. Bulk washing (10 g sample dipped in 1 litre tap water for 5 minutes) did not significantly reduce TPC and any of the pathogens. TPC decreased significantly by 1 log cycle up on treatment with 200 ppm NaOCl and 100 ppm KMnO₄. Staphylococcus aureus could be reduced more effectively with 100 ppm of KMnO₄; Listeria monocytogenes and Salmonella showed significant reductions (P<0.05) using both sanitizers. Shigella decreased by more than 1 log cycle when treated with 100 ppm KMnO₄.

Tap water is not sufficient to reduce the microbial load of fresh coriander. Use of 100 ppm NaOCl and 50 ppm KMnO₄ may be considered to reduce the pathogenic load of coriander leaves. Since reduction of pathogenic load by 1 log cycle is insufficient, other means of disinfecting the raw produce need to be tested. Also, use of good irrigation practices and aseptic post harvest techniques cannot be ignored.

This is an original study, wherein use of sanitizers should be promoted in order to reduce the microbial load of coriander leaves.

The present study is undertaken in the region of Guelma that is located in North-Eastern Algeria. Guelma’s agricultural plain is irrigated from the Seybouse ephemeral river (wadi). The latter collects the entire domestic and industrial wastewaters of the region that are discharged without prior treatment. Hence, the organic and biological or what so ever contributions to the wadi are capable of initiating a significant degradation of its waters’ quality and challenging their use for irrigation. The paper aims to discuss these issues.

The interpretation of the results of three sampling campaigns for chemical and bacteriological analyzes was carried out in terms of the indices’ method (organic pollution index (OPI) and microbiological quality index (MQI)). The results were statistically assessed through the use of the principal component analysis (PCA). The OPI was calculated according to the method of Leclercq and Maquet (1987) whose principle is to classify the contents for the polluting elements into 5 classes each class corresponding to a given range for the considered parameter, and then to calculate the overall average class number for each sample. The MQI depends on the water concentrations in: the total coliforms, the fecal coliforms, and fecal streptococci. One defines five classes of concentrations for each of these parameters. The MQI is again the overall mean of the numbers for the classes for every parameter.

The OPI reflects a moderate pollution for most sampling stations. The PCA indicates that the variables controlling salinity are mainly Cl⁻, Na⁺ and SO₄²⁻. In August 2016, El-Fedjoudj pumping station shows a very strong fecal contamination. According to the recommendations of World Health Organization (1989), waters from wadi Seybouse cannot be used for the irrigation of vegetable crops if they were to be consumed raw.

This study has an environment impact as it reveals the need to protect surface waters used for irrigation and therefore protect consumers of raw vegetables that are to be consumed uncooked.