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Chili pepper pods are a worldwide used and cultivated spice with a high economic and cultural importance. Bolivia is a center of origin and diversification of important crops including wild pepper varieties, but little is known about the chemical composition, antioxidant activity and the Scoville and bioactive compounds of these chili peppers. The purpose of this study was to contribute with new data about the chemical composition and the Scoville scale of Bolivian Chili peppers.

A total of 26 samples of Bolivian Chili peppers were extracted using distilled water and methanol. Total antioxidant capacity was determined by the ferric-reducing antioxidant power (FRAP) and by the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) methods. The total phenolic content was determined by Folin and Ciocalteu. Carotene, protein and ashes were determined by Bolivian analytical norms. Capsaicin was determined by high-performance liquid chromatography. All determinations were expressed as mean values± standard deviation of six replicates measured over three days of one extract. All values were less than 5% of coefficient of variation. Principal component analysis was applied to reveal patterns in the data. PC1 and PC2 together explained 78% of the total variance.

The protein content (3.8–6.3 g/100 g of dw) and the antioxidant activity (88.2 to 374 by FRAP and 87.0 to 172 evaluated by ABTS) studied in these species revealed that the values were above the average reported in the literature. The amount of pungency or capsaicin content expressed as Scoville Heat Units were reported moderately highly and very highly pungent (5,696–148,800 g dw) in several of the Bolivian peppers which demonstrates a good potential for capsaicin extraction and different applications for industry and as food ingredients.

This manuscript presents an important and novel contribution to the knowledge of the chili peppers in the region.

The etiology of fruit anthracnose in hot pepper (Capsicum frutescens) was investigated at Ibadan, Osogbo, and Ikenne in the lowland forest zone of western Nigeria. Collectotrichum capsici (Synd) Butler & Bisby was found associated with the fruit anthracnose of hot pepper in all locations. Out of 300 plants examined in all the locations, over 70 per cent had fruit anthracnose, while in some pepper fields all the fruits produced had the disease symptom. The pathogen overseasoned in pepper plant debris. A high inoculum population of 4.9×10⁶ g⁻¹ colony forming units/g was estimated in the soil of pepper fields. The seed from the infected hot pepper fruits also carried propergules of the pathogen. The fungus was also found on Lycopersicon esculentus, C. annum and Vigna unguiculata growing in and around the pepper fields. Pepper fruits infection by the disease occurs during the peak of the rainy season beginning in patches which spread later, resulting in extensive infection of the pepper field.

This study aims to deal with the evaluation of the antioxidant capacity of lyophilized hydroalcoholic extracts of red peppers in natura. Furthermore, preference was evaluated for the taste and color of soybean oil added red pepper extracts.

The antioxidant capacity was determined by four methods. The content of phenolic compounds, carotenoids and ascorbic acid in the extracts was determined by chromatographic, spectrophotometric and titration methods, respectively.

The results showed that the highest antioxidant capacity was found in Malagueta pepper extract through reducing power (FRAP) method. In this same extract, high amount of phenolic compounds was found. However, the extracts of Bode and Dedo-de-moça peppers had higher amounts of carotenoids and ascorbic acid, respectively. Sensorially, the oil added extracts were preferred.

Red peppers are very popular and consumed worldwide, besides being constituted of important phytochemicals. Results showed high antioxidant activity in the extracts of peppers, and high content of phenolic compounds, carotenoids and ascorbic acid mainly in chili. This study highlights the importance of the extracts of red peppers, genus Capsicum, as a source of antioxidants, in addition to vegetable oils.

It is important to check the acceptance of the application of extract in vegetable oil, so it can be marketed as a natural antioxidant. This study provides valuable information about the antioxidant capacity of extracts of red peppers and its acceptance.

Chilli pepper (Capsicum annum), an extensively cultivated vegetable, is commonly used to spice many dishes prepared in several parts of the world. It contains capsaicinoids. The most active amongst these capsaicinoids is capsaicin (8-methyl-N-vanillyl-6-nonenamide), which is neurogenic and so may affect nervous function. Therefore, the purpose of this paper is to investigate the effects of long-term consumption of chilli pepper and capsaicin diets on pain and social behaviour in CD-1 Swiss white mice.

In total, 30 male mice were randomly assigned into three groups of ten mice each, namely, control, pepper-diet (20 per cent w/w) and capsaicin-diet (10 per cent w/w) groups. Tail immersion, hot plate and formalin tests were conducted to assess pain perception, while nesting behaviour test was used to evaluate the social behaviour of the mice.

The latency of tail flick of both the pepper and capsaicin groups were significantly longer (p < 0.01 and p < 0.001, respectively) compared to control. The hind paw lick frequency, duration and flinching of both the pepper and capsaicin groups were also significantly reduced compared to control. The nesting score of the capsaicin group was significantly higher (p < 0.01) compared to control. However, the nesting score for pepper group was significantly lower (p < 0.05) compared to capsaicin group.

Long-term consumption of capsaicin and pepper diets suppressed pain and enhanced organized social behaviour in mice. One of the active principles responsible for the effects obtained with pepper on pain and social behaviour in mice may be capsaicin.

The growing consumer demand for microbiologically safe and quality products with sensory properties similar to those of natural products has spurred the search for natural flavourings with an antimicrobial effect on foods. The purpose of the present study is to evaluate the in vitro antimicrobial activity of “malagueta” chili pepper and “dedo de moça” hot pepper on Minas Frescal cheese supplemented with Escherichia coli strains.

Each cheese contained 0, 10, 15 and 20 per cent concentrations of “malagueta” chilli pepper and “dedo de moça” hot pepper supplemented with 200 µL of E. coli/kg cheese. The cheeses were stored under refrigeration at 7 °C for 28 days. The E. coli, pH and titratable acidity were analysed for this cheese.

“Dedo de moça” hot pepper showed a bacteriostatic effect on E. coli strains being more efficient on day 1. However, the “malagueta” hot pepper showed bactericidal effect and was efficient during the 28 days of storage. The pH showed a gradual decrease (p = 0.000) throughout the storage period; therefore, the acidity was increased even when the CFU/g count remained constant. The peppers had an antimicrobial effect on E. coli strains, and thus might be an alternative to extend the shelf life of Minas frescal cheese.

The study of natural condiments as an antimicrobial alternative is important because they prevent infections and food infections, increase shelf life and make it possible to offer differentiated products in the market.

The interests of Public Health in its medical aspect would seem to have always received support in the Union of South Africa. In the year 1911–12, for instance, the sum of one hundred thousand pounds was expended; in the year of the influenza epidemic three times that sum. The present rate of expenditure is in the neighbourhood of a quarter of a million. There are many public bodies who concern themselves with health conditions in the Union and they are all in touch with the central authority. The officials of the Public Health Department were eagerly waiting for this new Food and Drugs Act to become operative. The growth of industry in South Africa and its bearing on the future of the nation has been fully recognised if the statute book may be taken as a reliable guide. Thus the system of weights and measures was unified by the Act of 1923; the growth of industry encouraged by such Acts as that of the Iron and Steel Industry Encouragement Act of 1922; industrial machinery has been made to run more smoothly by the Industrial Conciliation Act, 1924, and the Wages Act, 1925. Public Health has been safeguarded by the creation of the Public Health Department and by the Public Health Act, 1919, and the Medical, Dental, and Pharmacy Act, 1928; but it was only six months ago that the Act under review came into operation, and the matter with which this Act is concerned lies at the very foundations of public health. The Bill was introduced by the Minister for Public Health on the 2nd February, 1928; and read for the second time on the 27th February. It received the cordial support of both Senate and House of Assembly. Not a dissentient voice was raised. Everyone was eager to support the urgent representations that had been made by such public bodies as the Chambers of Commerce and Industry, the Board of Trade and Agriculture, the Union Council of Public Health, and all the larger municipalities. The Bill had been drawn up after a careful study of similar Australian, New Zealand, and United States legislation. The existing Acts were hopelessly out of date. The Natal and Free State Acts had been founded on the Cape Province Act, and this in its turn on the English Act of 1875, so that legislation was over fifty years old at the time of repeal. Official figures showed that harmful adulteration might be as high as ten per cent. of the samples submitted and these figures certainly did not give a true idea of the extent of such adulteration. As to adulteration with non‐injurious substances it may be sufficient to state that 27 per cent. of butter samples taken in Cape Town contained from 11 to 6 per cent. of foreign fat. Coffee is almost a universal drink among the Dutch population of South Africa, but owing to the inadequacy of the laws the country had, in the words of a witness in Committee, become “ a dumping ground ” for coffee of such an inferior kind that it is difficult to imagine anyone who could get anything better drinking it. Nevertheless it was described by the vendors in such glowing terms as to call forth protests from the Brazilian consul. Sometimes “ coffee ” was not coffee at all. It might be “ banana skins !” Sometimes it was worse than this. Such was a consignment of coffee from Hamburg. It had been in store for more than two years, and its first use was to nourish a large population of weevils—we understand that coffee must be two years old and over before this can happen. These creatures had made such good use of their opportunity that not much was left of the original coffee. As such stuff was only fit for the rubbish destructor it went to South Africa. The bits of beans plus weevils were embedded in a clay matrix, of the proper shape, to give them coherence, baked, stained, and polished—by the way it may be said that the staining and polishing of coffee had by this time assumed the character and dimensions of a skilled industry. Fortunately at this stage of the proceedings the health authorities at Cape Town intervened. It was stated by the Minister who introduced the Bill, with some reserve, that the incidence of adulteration had reached such proportions that the commercial morality of the Union in general was beginning to deteriorate. We should think so. There was certainly little to encourage the ordinary trader to put Sunday school maxims into practice. Fortunately public patience broke down before public health. On the 1st March the Bill was read for the first time in the Senate. It went into the Committee stage on the 8th. Here the usual revelations were made. Milk had, of course, received its full share of attention. So much so indeed that the Act forbids a milk vendor to carry skim milk or water in the same cart when delivering whole milk. It also appeared in evidence that a dairyman had only to keep two or three cows, which yielded inferior milk, in his herd, water the milk of the lot and plead the cow, when prosecuted, with impunity. More than that such a cow was actually hired out to another milk vendor, whom the authorities were wicked enough to prosecute, so that he might take advantage of the peculiarities of the animal and the weakness of the law. It is said that Huxley had great faith in the elasticity of the Hebrew language in the hands of Biblical commentators, it cannot surpass our belief in the almost infinite possibilities of the cow when milk prosecutions are “ going,” but this new use for old cows had not occurred to us. An important witness stated that in his opinion the 3 per cent. minimum for fat in milk is very low, very little lower indeed than the average standard for milk in Cape Town. Cape Town milk it seems is poorer in fat than up country milk. This has been attributed to the Friesian cattle as in “ short horn ” districts, the fat percentage is always higher. Nevertheless Act No. 13, 1929, Chap. II., Part C. s. 17 (3) still declares the minimum fat content for milk sold for domestic purposes to be 3 per cent. Thus, it seems to us, a good opportunity of raising the minimum legal fat content to the great benefit of everybody has been missed. Most assuredly it will not readily recur. No doubt there would have been strong opposition on the part of the trade had any attempt been made to raise this low standard. There always has been. If we had had any doubt on the subject of trade opposition that doubt would have been removed by the following. The same witnesses stated that all the best brands of herds in the Cape Peninsular, are tested for tuberculosis which is “ very prevalent. ” He agreed that milk from tuberculous cows was “ highly dangerous to infant life. ” In reply as to whether it would not be safer to have all herds compulsorily tested, he said: “ It is a question they are afraid to tackle. ” They have been at it for the last 25 years. “ Q. What is the reason? Is it because ” tuberculosis is too prevalent in the Cape A. “ No. I think it is because it affects so many people. “ Had they started it 25 years ago there would not have been this trouble to‐day. “ During the past few years manufacturers of fruit juices and the like had written asking for particulars of food standards and enclosing copies of analyses. It had to be stated in reply that there were no food standards, but that a draft Food and Drugs Bill had been prepared and would probably be before the House during the next session. The Assistant Health officer of the Union who made this statement added, “ I have had to resort to this method of excluding adulterated food for the last three or four years and cannot carry on much longer. ” To send fruit juices to the land of fruit seems rather like sending coals to Newcastle. However, the addition of pectinous matter to preparations of fruits naturally deficient in pectin is well known, necessary, and permissible. But if this be done with the object of overloading, a jam declared to be made of one kind of fruit with a cheaper undeclared pulp it is a fraud which the Act is drawn to prevent. Chap. V. s. 42 empowers the Minister to make regulations under the Act and publish them in the Gazette. In the issue of the 28th March, p. 9, “jam” is defined. No mineral acid, flavouring substance, nor any vegetable substance save that derived from the varieties of fruits named on the label are permitted, but the jam may contain “a trace” of fruit‐derived malic, citric or tartaric acid, colouring matters as scheduled (p. 4) and added pectin not exceeding 0·3 per cent. calculated as calcium pectate. In “Fruit jelly” this may be 0·6 per cent. It is evident that without this regulation a consumer in this country of South African fruit products would have had no assurance that he was not getting synthetic products of European manufacture in South African fruit tins. As a last instance of the ease with which the law might be evaded and adulteration practiced the following may suffice. An inspector in the Cape Province asked for some “ mixed coffee. ” It was supplied him labelled “ mixed coffee ” with a verbal intimation that it contained 25 per cent. of chicory. It did, and 10 per cent. of ground acorns in addition. The conviction which followed was quashed on appeal by Mr. Justice Solomon on the grounds that acorns cost as much as chicory, that they were not shown to have been added to fraudulently increase the bulk, and that there was no evidence that acorns were injurious to health. It need hardly be said that this decision, extra‐ordinary thought thought it may seem, was in strict accordance with the letter of the law in this case, presumably ss. 6 and 7 of the Cape Province Act. Readers who may have followed us so far will probably by this time have come to the conclusion that any change in the law would have been for the better in the interests of the public health and the commercial reputation of the Union. Moreover as the instances of rascally practice that we have cited do not seem to have been at all “ out of the way, ” the successful continuance of such practice under what really amounted to legal protection would induce a belief that the people who would put up with such things must, in the words of Oriental euphemism, be “ afflicted of God ”; and belief in the existence of this unhappy state of things would have been considerably strengthened by the knowledge that at the very time they were spending thousands every year in the interests of public health, the Department of Public Health itself was almost hopelessly oppressed by the incubus of sub‐fossil legislation fifty years behind the times. That while the country was being advertised as a tourist ground and health resort no one from Cape Town to Johannesburg could be sure that any food product he might buy would not be grossly and harmfully adulterated. That while they were building up an extensive overseas trade in foodstuffs they were content to eat and to drink any rubbish that might be foisted on them. While the delay of the Government in amending the law and so putting an end to a state of things that had apparently become a sort of public scandal is hard to understand. It has taken fourteen years! We recall the action of Mr. Snodgrass in the street row in Ipswich who “ in a truly Christian spirit, and in order that he might take no one unawares, announced in a very loud tone that he was going to begin, and proceeded to take off his coat with the utmost deliberation. ”

The following definitions and standards for food products have been adopted as a guide for the officials of this Department in enforcing the Food and Drugs Act. These are standards of identity and are not to be confused with standards of quality or grade; they are so framed as to exclude substances not mentioned in the definition and in each instance imply that the product is clean and sound. These definitions and standards include those published in S. R. A., F. D. 2, revision 4, and those adopted October 28, 1936.

Results of experiments by research experts in the food value of canned foods will shortly be published by the Ministry of Agriculture. According to the Ministry the point has now been reached when canned foods may be said to sell on their own merits, and not as a mere substitute for fresh foods. The most obvious attribute of canned foods was that they made available a permanent supply of foodstuffs which were otherwise limited to a season, as well as making available to consumers fruits which could not otherwise be obtained in their natural state. In view of the wide range of varieties of canned foods and vegetables now available any generalised statement as to their value was impracticable, but it might be broadly stated that their energy‐producing value, as expressed in calories, was never inferior to that of the same kinds for consumption fresh, or in some other prepared form. Recent research had shown that vitamins were not necessarily destroyed by canning, and indeed some canned foods—for instance, canned tomatoes—might be very nearly as rich in vitamins as the raw product. An outstanding example of the importance of the canned food industry was the market which had been created for British fresh picked peas. Here the farmers had profited by an expanding but controlled increase of acreage under crop, with prices remaining very stable for the last few years. It was probable that the same general tendency would be observable with plums, and with most other canning crops, as the industry developed. In this country an increased consumption of home‐canned goods, if secured at the expense of imported canned goods, or some other imported agricultural commodity, would mean that a new market had been created for British growers, while a similar benefit would be obtained if export markets were developed. This would not be true if home‐canned goods replaced other home‐grown crops, but in this case it might mean a change‐over from an unprofitable to a profitable crop.

This study aims to treat the development and application of sequence characterised amplified region (SCAR) markers for the detection of plant based adulterants (dried red beet pulp and powdered Ziziphus nummularia fruits) in traded ground chilli.

Adulterant‐specific DNA fragments (red beet pulp specific – “Beet 01” and Z. nummularia specific – “Ziz 01”) identified by random amplified polymorphic DNA polymerase chain reaction (RAPD‐PCR) analysis were cloned and sequenced for SCAR marker development. Red beet pulp specific SCAR primer pair, B1, and Z. nummularia specific SCAR primer pair, Z1, were designed from the corresponding RAPD marker sequences to amplify SCAR markers of 320 bp and 389 bp, respectively. The utility of the SCAR markers for adulterant detection was verified in model blends of chilli powder with the adulterants. Six commercial samples of ground chilli powder were analysed using the SCAR markers.

SCAR markers could detect the adulterants at a concentration as low as 10 g adulterant kg⁻¹ of blended sample. The Z. nummularia SCAR marker could detect the presence of Z. nummularia fruit adulteration in one of the commercial samples. All the market samples tested were free from red beet pulp adulteration.

The PCR‐based method developed in the study is simple, rapid, and sensitive and has the potential to be developed into a quantitative analytical method and for commercial PCR kits for the large‐scale screening of ground chilli to detect and prevent plant‐based adulterants. The work has public health significance too, as ground chilli is one of the major spices consumed worldwide.

The study is the first report on the development of SCAR markers for adulterant detection in ground chilli. This work has relevance, as adulteration is a major concern of the sanitary and phytosanitary issues of the World Trade Organization (WTO) agreement.

Literature has consistently reported that coloured plants are rich in dietary bioactive compounds. Therefore, this study aims to compare the total phenolic content and antioxidant activities in selected coloured plants (blue butterfly pea flower, roselle calyx, yellow bell pepper and purple sweet potato).

Total Phenolic Content (TPC) was determined using Folin–Ciocalteu assay, while antioxidant activities were evaluated using 2,2-dophenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging, Ferric Reducing Antioxidant Power (FRAP) and Trolox Equivalent Antioxidant Capacity (TEAC) assays.

TPC was reported from 273.15 ± 19.57 µg GAE/g DW (blue butterfly pea flower extract) to 363.10 ± 7.94 µg GAE/g DW (roselle calyx extract). Antioxidant activities as determined by DPPH assay ranged from 17.26 ± 0.06% (purple sweet potato extract) to 83.38 ± 1.04% (yellow bell pepper extract); while for FRAP assay was 4.92 ± 0.18 mg Fe (II)/g DW (purple sweet potato extract) to 128.33 ± 11.59 mg Fe (II)/g DW (roselle calyx extract). On the other hand, TEAC values were in the range of 15.26 ± 2.83 µg Trolox/g DW (roselle calyx extract) to 364.27 ± 7.14 µg Trolox/g DW (blue butterfly pea flower extract). A significant moderate positive correlation was observed between TPC and DPPH (r = 0.562) as well as TPC and FRAP (r = 0.686).

This study was the first to compare the total phenolic content and antioxidant activities in coloured plants. Findings derived from this study can be extended to the formulation of natural food colourants and nutraceuticals.