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11 – 20 of 694Melis Asal, Özlem Özen, Mert Şahinler, Hasan Tahsin Baysal and İlker Polatoğlu
Traditional analytical methods are often time-consuming and require bulky instruments, making their widespread implementation challenging. This paper aims to represent the…
Abstract
Purpose
Traditional analytical methods are often time-consuming and require bulky instruments, making their widespread implementation challenging. This paper aims to represent the principal concepts of biosensors as an introduction of this technology to readers and offers a comprehensive understanding of its functions.
Design/methodology/approach
The authors provide descriptions of the components, characteristics and advantages of biosensors along with the immobilization methods, followed by a brief discussion.
Findings
A biosensor is an analytical device comprising a specific biomolecule and a transducer in conjunction with an output system. The biomolecule recognizes a specific target which leads to a change in physicochemical properties of a system. This biorecognition phenomenon is later converted into a detectable signal by the transducer. Biosensors can essentially serve as rapid and cost-effective devices with excellent sensitivity and specificity for critical purposes in innumerable fields, ranging from scientific research to day-to-day applications.
Originality/value
Here, the authors explain and discuss the approaches and challenges with the aim of leading to an interest in biosensor development and improving their applications.
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Bushra Rafique, Mudassir Iqbal, Tahir Mehmood and Muhammad Ashraf Shaheen
This review aims to focus on recent reported research work on the construction and function of different electrochemical DNA biosensors. It also describes different sensing…
Abstract
Purpose
This review aims to focus on recent reported research work on the construction and function of different electrochemical DNA biosensors. It also describes different sensing materials, chemistries of immobilization probes, conditions of hybridization and principles of transducing and amplification strategies.
Design/methodology/approach
The human disease-related mutated genes or DNA sequence detection at low cost can be verified by the electrochemical-based biosensor. A range of different chemistries is used by the DNA-based electrochemical biosensors, out of which the interactions of nanoscale material with recognition layer and a solid electrode surface are most interesting. A diversity of advancements has been made in the field of electrochemical detection.
Findings
Some important aspects are also highlighted in this review, which can contribute in the creation of successful biosensing devices in the future.
Originality/value
This paper provides an updated review of construction and sensing technologies in the field of biosensing.
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Amin Eidi, Mousa Shamsi and Habib Badri Ghavifekr
In this work, the sensing and actuating elements are designed with interdigitated capacitors away from the sensitive element on which the droplet is placed. This pattern helps to…
Abstract
Purpose
In this work, the sensing and actuating elements are designed with interdigitated capacitors away from the sensitive element on which the droplet is placed. This pattern helps to prevent interference of electrical elements with the droplet. Choosing shear resonance mode at this proposed structure minimizes the damping effect of droplet touch by the resonator structure. The glass-based standard fabrication method of the proposed biosensor is presented exactly.
Design/methodology/approach
Mechanical resonator sensors are extremely limited because of the high damping factor and the high electrical conductivity in the aqueous environment. In this work, a molecule detector biosensor is proposed for droplet analysis, which is possible to fabricate using micro-electro-mechanical systems (MEMS) technology. By electromechanical coupling of resonators as a mechanical resonator structure, a standing mechanical wave is formed at this structure by electrostatic actuating elements.
Findings
In this paper, a mechanical resonator structure as a biosensor is proposed for micro-droplet analysis that can be fabricated by MEMS technology. It is designed at a lower cost fabrication method using electrostatic technology and interdigitated capacitors. The response of the biosensor displacement frequency at the resonance frequency of the desired mode is reasonable for measuring the capacitive changes of its output. The mass sensitivity of the proposed biosensor is in the range of 1 ng, and it has a large sensitive area for capturing target molecules.
Originality/value
To evaluate the quality of the proposed design, the stimulated analysis is conducted by COMSOL and results are presented.
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This paper considers the role of biosensors for monitoring the environment. A selection of existing products and competitive techniques such as biochemical assays and laboratory…
Abstract
This paper considers the role of biosensors for monitoring the environment. A selection of existing products and competitive techniques such as biochemical assays and laboratory analysis which presently dominate the business are discussed. A number of research activities and themes are considered. Conclusion are drawn on the prospects for environmental biosensors.
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Biosensors have been described as a synergistic combination of biochemistry and microelectronics.
Mulayam Singh Gaur, Rajni Yadav, Mamta Kushwah and Anna Nikolaevna Berlina
This information will be useful in the selection of materials and technology for the detection and removal of mercury ions at a low cost and with high sensitivity and selectivity…
Abstract
Purpose
This information will be useful in the selection of materials and technology for the detection and removal of mercury ions at a low cost and with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity.
Design/methodology/approach
Different nano- and bio-materials allowed for the development of a variety of biosensors – colorimetric, chemiluminescent, electrochemical, whole-cell and aptasensors – are described. The materials used for their development also make it possible to use them in removing heavy metals, which are toxic contaminants, from environmental water samples.
Findings
This review focuses on different technologies, tools and materials for mercury (heavy metals) detection and remediation to environmental samples.
Originality/value
This review gives up-to-date and systemic information on modern nanotechnology methods for heavy metal detection. Different recognition molecules and nanomaterials have been discussed for remediation to water samples. The present review may provide valuable information to researchers regarding novel mercury ions detection sensors and encourage them for further research/development.
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Hamza Usman, Muhammad Hafiz Abu Bakar, Amir Syahir Hamzah and Abu bakar Salleh
This paper aims to estimate the level of histamine in fish and fish products, as it is very important because of their implication in fish poisoning in humans; hence, ascertaining…
Abstract
Purpose
This paper aims to estimate the level of histamine in fish and fish products, as it is very important because of their implication in fish poisoning in humans; hence, ascertaining histamine levels in the aforementioned serves as a chemical index for spoilage.
Design/methodology/approach
A technique was developed to immobilize an ordered multilayer of diamine oxidase (DAO) by means of chemical cross-linking on the biconical taper surface stepwisely alternating between chitosan, glutaraldehyde and the enzyme. A spectrophotometric signal results from horseradish peroxidase catalyzed reduction of H2O2, a secondary product of the oxidative deamination of histamine monitored at 450 nm.
Findings
The biosensor showed a linear response range up to 1.5 mM, a good sensitivity of 0.64 mM-1 with detection and quantification limits towards histamine of 0.086 mM (15.8 ppm) and 0.204 mM (37.7 ppm) and a linear response range of 0-1.5 mM. It showed a response and recovery time of 14 sec and operational stability up to 40 repeated analyses without significant loss of sensitivity.
Practical implications
The developed biosensor has a good potential for use in the quantitative determination of histamine in seafood.
Originality/value
The paper described an outcome of an experimental work on tapered fibre optics (taper)-based biosensor coated with DAO embedded into a chitosan membrane to measure histamine.
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J.D. Newman, L.J. Tigwell, P.J. Warner and A.P.F. Turner
Describes the basic principles of biosensors and the history of their development. Assesses their current impact and state of the biosensor market, and looks ahead to future…
Abstract
Describes the basic principles of biosensors and the history of their development. Assesses their current impact and state of the biosensor market, and looks ahead to future developments.
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Nurul Hazwani Daud, Siti Nurbaya Oslan, Thean Chor Leow and Abu Bakar Salleh
The main purpose of this study is to demonstrate the development of an enzyme-based sensor for haloalkane detection. Haloalkane is a toxic compound that is found as genotoxic…
Abstract
Purpose
The main purpose of this study is to demonstrate the development of an enzyme-based sensor for haloalkane detection. Haloalkane is a toxic compound that is found as genotoxic impurities in pharmaceutical products and contaminants in waste. The need to investigate the genotoxic level in pharmaceutical manufacturing is very crucial because of its toxicity effects on human health. The potential of mini protein as an alternative bioreceptor was explored with the aim to be more effective and stable under extreme conditions.
Design/methodology/approach
Mini proteins of haloalkane dehalogenase (HLD) were computationally designed and experimentally validated. Tapered multimode fiber (TMMF) was bio-functionalized with a bioreceptor either native (positive control) or mini protein. The absorbance-based sensor resulting from the binding interaction of mini protein with haloalkane was monitored through a wavelength range of 200-1,300 nm.
Findings
An increment of the UV absorption is observed at 325 nm when haloalkane interacted with the immobilized bioreceptors, native or mini protein. Both biosensors displayed a continuous response over the range of 5-250 µM haloalkane. They also had the capability to detect haloalkanes below 1 min and with an operational stability of up to seven days without significant loss of sensitivity.
Practical implications
The results indicate the potential viability of the enzyme HLD-based sensor to monitor the existence of haloalkane in both pharmaceutical and environmental products.
Originality/value
The paper describes an outcome of experimental work on TMMF-based biosensor coated with HLD for label-free haloalkane detection. Mini protein can be used as an effective bioreceptor with some structural modification to improve functionality and stability.
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Biosensors are reaching the stage of serious evaluation and exploitation, as well as unusual applications, as Stephen McClelland explains.