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Disinfecting laundry processes are essential to avoid contamination of laundering machines and linen during commercial laundry reprocessing in the health care sector. Recently a bacteriophage‐charged bioindicator has been developed using MS2 as surrogate virus for testing of low‐temperature disinfecting laundry processing on efficacy against viruses related to practice. This paper therefore aims to investigate application of MS2‐bioindicators in chemothermal processes under practical conditions (phase 2/step 2) and in practice (phase 3).

The experimental design was developed and modified according to the German Society for Hygiene and Microbiology (DGHM) Standard Methods for Testing Chemical Disinfection Processes. Tests under practical conditions were performed at 60°C and 70°C. Additional tests in tunnel washers were carried out at 60°C and 70°C. In all experiments validated disinfecting laundry processes, recommended for bactericidal and virucidal performance (categories A and B), were applied.

The results show a temperature‐dependent gradual efficacy against the test virus MS2 up to reduction values of more than 8 log₁₀‐steps. Therefore MS2‐bioindicators prove to be suitable as a tool to determine the performance of disinfection procedures against viruses in practice.

Phage‐charged bioindicators may be a tool to provide further insights into the reliability of antiviral laundry processes for health care quality management and for infection control.

Determining minimum water flows that will continuously run through a natural channel downstream of a water intake (Q0) is a multidisciplinary problem. It is, however, not approached in a multidisciplinary way in most countries. This paper offers an overview of the main methods and proposes a practical and environmentally more acceptable way of determining the retained flow, namely the environmentally acceptable flow (EAF). It is proposed that until the conditions are created for use of more complex methods and/or biological response techniques, the EAF be defined by determining whether the retained flow is sufficient for sustaining and developing indigenous wildlife in the streams by ensuring of the essential living conditions for the bioindicator species. The proposed bioindicators are characteristic fish populations of a specific type of stream and stream reaches. Being the final link in the ecological food chain, fish species are reliable indicators of bioecological balance in a stream.

Purpose – The purpose of this research is to examine the diversity of macrozoobenthos as well as its relationship with water quality and substrate in the pond culture area.

Design/Methodology/Approach – The method of sampling area is on five observation stations by purposive sampling. The research was done indirectly (ex situ) for macrozoobenthic identification at the Ecology Laboratory, Faculty of Mathematics and Natural Sciences.

Findings – The fairness/uniformity index obtained from the five research stations ranging from 0.483 to 0.923 indicates a high degree of uniformity. This indicates that the macrozoobenthos biological index at the study site can be used as an indicator that water quality is in good condition and has the potential to be developed into an aquaculture area as well as supporting the success of the shrimp farming as one of the sub-systems of the shrimp agribusiness.

Research Limitations/Implications – This research can be a source of information for the management and utilization of environment in the research area, so that shrimp harvest can be optimized in the pond farming area.

Originality/Value – This research has found that macrozoobenthos included 61 species.

In this paper, we discuss methods to integrate ecological resources, ecosystem services, risk, and the transition to long-term stewardship on Department of Energy lands. Three types of information are required about ecological resources before decisions can be made about remediation, site transitions, and long-term stewardship: (1) the ecological resources and ecosystem functions (such as productivity) present on site and their spatial pattern, (2) the ecosystem services these resources provide to people, and (3) the risks from the interactions between people and these ecosystems. Once the ecological resources and ecosystem services are evaluated, then decisions about future land use, preservation, conservation, or protection of ecological resources within a designated land use can be implemented. Long-term stewardship requires both ecosystem protection in terms of biological resources and ecosystem function as well as biomonitoring to ensure minimal radiological or chemical risk and to inform future management. In some cases, protection of ecological resources may be preferable to cleanup that is physically disruptive, provided land use designation is consistent with ecological protection. In such instances, less site cleanup can prove preferable to more.

The goal of this paper is to detect helminth eggs in treated wastewater for sanitary control, using them as biological indicators of wastewater quality.

Samples were taken from the Municipal Wastewater Treatment Plant of the Metropolitan Area. To concentrate the samples, centrifugation‐flotation on 33 percent zinc sulphate was applied.

The study revealed the constant presence of helminth eggs as Ascaris sp., Enterobius sp., Hymenolepis sp., and Strongyloides sp.

The results indicate that helminth eggs can be used as bioindicators for sanitary control of treated wastewater to be reused in urban areas.

Monitoring air, water and soil contamination by means of animals, plants, fungi and bacteria is increasingly in demand for assessing environmental quality, environmental impacts, and health and safety. It is essential that environmental monitoring by bioassays is credibly based on standardized procedures and on substantial scientific evidence which should include background measurements to establish baseline data. Cites case studies involving the use of organisms for monitoring heavy metals and radionuclides in order to demonstrate such procedural pre‐requisites in environmental surveillance.

The purpose of this paper is to describe the case of the Valorbio research project, in which students of different high-level programs were involved in the experimental work and in the dissemination of results in collaboration with the research team.

The inclusion in higher education curricula of content related to the sustainable development should be a preferred mechanism for the dissemination of good practices of sustainability. Another equally important way to achieve this is to involve students in research projects that seek solutions to the societal challenges related to sustainable growth. The Valorbio project aims to meet the needs for treating and reusing wastewater and solid waste. Its main goal was the development of modular systems for wastewater treatment based on constructed wetlands, exploring the possibility of the treatment systems being composed of solid waste and by-products from significant industrial sectors.

The students’ contribution to the research work was relevant and simultaneously allowed them to acquire skills on sustainable development. Additionally, the students contributed to the dissemination of the results. The Valorbio project can thus be considered a successful application of the concept of project-based learning (PBL), as a way to include sustainability issues content in the higher education curricula.

The applied experimental work had an original approach regarding the equipment design, the waste materials valuation, as well as the integration of waste treatment processes in the circular economy paradigm. This paper is the first reported PBL experience involving students of short-cycle technical–professional programs in partnership with first and second-level students and a research team.

Nature-based solutions (NBS) generate different impacts at the urban scale, such as the ability to regulate water or store carbon, comparable to traditional, gray infrastructures in a more cost-efficient way. On the other hand, by their intrinsic nature, NBS do deliver a series of other services that are commonly defined as social, economic, and environmental cobenefits. These benefits are not always valued in a consistent and complete way, so there is the need to compile a more comprehensive evidence base on the social, economic, and environmental effectiveness of NBS. The chapter attempts to identify a categorization of the existing NBS and define the ecosystem services (ES) provided by them. Furthermore, starting from the results achieved through the definition of the existing NBS frameworks assessment, the chapter will identify a set of key performance indicators KPIs, based on the ES produced by NBS, to measure the economic, social, and environmental benefits generated in by NBS at the urban level taking into account their multifunctional character. In total, 66 key performance indicators have been individuated: 3 for provisioning services, 38 for regulating services, 17 for cultural services, and 8 for supporting services. Each indicator has been associated to a category of ES in order to measure and evaluate the performances of NBS implemented in cities.

Systems theory and open systems principles trace their origins to the life sciences. Our observations of living systems also inform the design and management of sustainable communities and organizations. Grounded in the patterns of living systems and social ecologies, the stewardship design principles (SDP) – balance, interdependence, regeneration, diversity, and succession (BIRDS) – can increase the agility of sustainable design practitioners in ramping up from small-scale experiments to large-scale systems change. The urgency of addressing global challenges such as climate change calls upon social change practitioners – be they business leaders, social entrepreneurs, or both – to create and/or adapt tools to increase the velocity and range of positive social change. Case vignettes in the design of small-scale experiments illustrate how the application of stewardship design principles can help expedite larger systemic change at the regional, statewide, and national levels.