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Crops are increasingly affected by drought; hence, the current study explored the potential role of three desert endophytic fungi, Aspergillus fumigatus, Aspergillus terreus and Talaromyces variabilis, in conferring drought tolerance in tomato plants.

Preserved endophytic fungi from a Rhazya stricta desert plant were adopted to obtain the required fungal treatment; tomatoes received fungal treatments directly in plastic trays and subsequently in pots. Drought was applied using 15% of PEG-6000 at two stages: flowering and fruiting. The following parameters were measured: pollen sterility, growth characteristics, morphological analysis and biochemical analysis, including proline, gibberellic acid (GA3) and chlorophyll measurements; thus, the data were analyzed statistically using SPSS software.

All applied endophytes significantly promoted pollen viability and tomato yield under stressed and nonstressed conditions. Interestingly, these endophytes significantly enhanced the number of trichomes under drought stress and promoted tomato fruit quality. The colonized tomato plants accumulated a high proline level under drought stress but lower than un-inoculated stressed plants. Also, a significant rise in growth characteristics was observed by A. fumigatus and A. terreus under normal conditions. Moreover, both raised GA3 levels under drought-stressed and nonstressed conditions. Also these two endophytes enhanced chlorophyll and carotenoid contents under drought stress. Fruit characteristics were enhanced by nonstressed T. variabilis and stressed A. fumigatus.

The present endophytic fungi provide impressive benefits to their host in normal and drought-stressed conditions. Consequently, they represent valuable sources as sustainable and environmentally friendly alternatives to mitigate drought stress.

Acrylamide is a compound found in several food products. Due to the toxicity of this compound, research also seeks strategies to modify industrial and homemade processes, impacting on the reduction of the compound. This paper aims to discuss the aspects surrounding the presence of acrylamide in foods.

Published literature on the presence of acrylamide in foods and on its effects has been reviewed. This paper explores the importance of this compound, summarizes the knowledge of its formation and gathers data on its incidence in food and the possibilities of mitigation. Special attention is given to an evaluation of the toxicological tests applied, to analyze whether acrylamide can be considered as a food safety problem.

Human exposure to food with high levels of acrylamide varies in their levels regarding the consumption of food in the diet and not only by the level of the compound present in them. Although the compound is well defined as toxic to humans, the association between its intake and most common cancers may not be directly related.

Depending on the approach of the researchers, contradictory results are obtained, showing the importance of this topic to the development of healthy food products. Further research is still needed to validate the potential effects of acrylamide on human health.

This paper aims to evaluate the bioremediation [chemical oxygen demand (COD) and color removal] of the effluent from the cardboard recycling industry in Yazd, central province of Iran, using mixed fungal culture.

First, the effluent samples from the cardboard recycling industry were cultured on potato dextrose agar medium to isolate native fungal colonies. The grown colonies were then identified using morphological macroscopic and microscopic characteristics to choose the dominant fungi for bioremediations. The mixed cultures of Aspergillus niger, Aspergillus flavus and Penicillium digitatum were finally used for bioremediation experiments of the cardboard recycling industry. A suspension containing 1 × 10⁶ CFU/ml of fungal spores was prepared from each fungus, separately and their homogenous mixture. Sewage samples were prepared and sterilized and used at 25%, 50% and 90% dilutions and pH levels of 5, 7 and 8 for bioremediation tests using mixed fungal spores. Following that, 10 ml of the mixed fungal spores were inoculated into the samples for decolorization and COD removal and incubated for 10 days at 30°C. The amount of COD removal and decolorization were measured before incubation and after 3, 6 and 10 days of inoculation. In this research, the color was measured by American Dye Manufacturer Institute and COD by the closed reflux method. The results of the present study were analyzed using SPSS 21 statistical software and one-way ANOVA tests at p-value < 0.05.

The results of this research showed that the mean decolorization by mixed fungal culture over 10 days at pH levels of 5, 7 and 8 were 44.40%, 45.00% and 36.84%, respectively, and the mean COD removal efficiency was 71.59%, 73.54% and 16.55%, respectively. Moreover, the mean decolorization at dilutions of 25%, 50% and 90% were 45.00%, 31.93% and 30.53%, respectively, and the mean COD removal efficiency was 73.54%, 62.38% and 34.93%, respectively. Therefore, the maximal COD removal and decolorization efficiency was obtained at dilution of 25% and pH 7.

Given that limited studies have been conducted on bioremediation of the effluent from the cardboard recycling industry using fungal species, this research could provide useful information on the physicochemical properties of the effluent in this industry.

The purpose of this paper is to optimize production of cellulase enzyme from agricultural waste by using Aspergillus fumigatus JCF. The study also aims at the production of bioethanol using cellulase and yeast.

Cellulase production was carried out using modified Mandel’s medium. The optimization of the cellulase production was carried out using Plackett-Burman and Response surface methodology. Bioethanol production was carried out using simultaneous saccharification and fermentation.

Maximum cellulase production at optimized conditions was found to be 2.08 IU/ml. Cellulase was used for the saccharification of three different feed stocks, i.e. sugar cane leaves, corn cob and water hyacinth. Highest amount of reducing sugar was released was 29.1 gm/l from sugarcane leaves. Sugarcane leaves produced maximum bioethanol concentration of 9.43 g/l out of the three substrates studied for bioethanol production.

The present study reveals that by using the agricultural wastes, cellulase production can be economically increased thereby bioethanol production.

The purpose of this paper is to speak about the production of biodiesel from waste cooking oil which serves as an alternate fuel in the absence of conventional fuels such as diesel and petrol. Though much research work was carried out using non-edible crops such as Jatropha and Pongamia, cooking oil utilized in bulk quantity is discarded as a waste. This is reused again as it contains more of esters that when combined with an alcohol in presence of an enzyme as a catalyst yields triglycerides (biodiesel).

The lipase producing strain Rhizopus oryzae and pure enzyme lipase is immobilized and treated with waste cooking oil for the production of FAME. Reaction parameters such as temperature, time, oil to acyl acceptor ratio and enzyme concentration were considered for purified lipase and in the case of Rhizopus oryzae, pH, olive oil concentration and rpm were considered for optimization studies. The response generated through each run were evaluated and analyzed through the central composited design of response surface methodology and thus the optimized reaction conditions were determined.

A high conversion (94.01 percent) was obtained for methanol when compared to methyl acetate (91.11 percent) and ethyl acetate (90.06 percent) through lipase catalyzed reaction at oil to solvent ratio of 1:3, enzyme concentration of 10 percent at 30°C after 24 h. Similarly, for methanol a high conversion (83.76 percent) was obtained at an optimum pH of 5.5, olive oil concentration 25 g/L and 150 rpm using Rhizopus oryzae when compared to methyl acetate (81.09 percent) and ethyl acetate (80.49 percent).

This research work implies that the acyl acceptors methyl acetate and ethyl acetate which are novel solvents for biodiesel production can also be used to obtain high yields as compared with methanol under optimized conditions.

The pursuit of manufacturing new inks with low financial cost is an urgent economic demand. Thus, the purpose of this paper is to synthesize some new pigments derived from Lithol Rubine (LR) via a successful simple route and to investigate their physicochemical properties for usage in the inks industry.

Two novel pigments were generated during the reaction of LR with Mn(II) and Co(II) salts in ethanolic solutions. The obtained pigments were isolated as solid compounds and characterized through elemental analysis, UV–vis, Fourier transform infrared, 1H NMR spectra, oil absorption, specific gravity, melting point, molar conductivity and magnetic moment measurements. Their dyeing and durability characteristics were examined using American Standard Testing Methods. The synthesized pigments were then applied in inks formulation.

The printing inks containing the two new pigments (LR–Mn and LR–Co) were compared to (GF 59-606 and GF 59-616), respectively. The results of this study showed that the performance of newly prepared pigments was comparable to that of commercial pigments currently in use in the inks industry.

LR and its new derivative pigments can be used in other different applications such as paper coating, crayon, rubber and paint industries.

The authors designed an efficient synthesis for some novel pigments. The synthesis technique is featured by a short reaction time, high yields and ease of use. The pigments developed would be good and cost-effective substitute for the original commercially available and expensive pigments used in the inks industry.

This paper is a systemic review on enset plant’s role in Ethiopian people’s life as the source of food. This paper aims to summarize the traditional processing and preparation methods of enset-based foods and their nutritional composition.

Available scientific articles were collected and reviewed for enset plant evaluation, description, enset plant’s role in Ethiopian people’s food security, post harvesting and traditional processing of enset plants, microbiology of the fermented enset foods, different foods reported from enset, nutritional profile of the three food from enset base (kocho, bulla and amicho) and other non-food applications of enset plant.

Enset plant has a predominant role in people living in the southern part of Ethiopia. This plant is drought-tolerant and provides many non-food applications. Harvesting of the enset plant, preparing for fermentation and food preparations follow the traditional route by using the indigenous knowledge and practices. Limited studies have been reported on the microbiology of the enset fermentation, but various types of microbes have been reported. In case of nutritional composition, foods from enset are reported to contain high carbohydrate and minerals content, such as calcium, potassium and zinc, but limited protein content; they are also the best source of the essential amino acids such as lysine and leucine. Limited data are available on vitamins, anti-oxidant and fatty acids profiles of enset-based foods. The existing data indicate variations, and the reasons for variability are discussed in this paper.

Scientific reviews on enset food nutrition profile and related issues are scarce; this paper will compile information about enset plant-based foods for researchers for their future research.