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Article
Publication date: 4 October 2019

Kholowd AlKhaldi, Manal Daghestani and Thanaa Al-Haddad

The purpose of this paper is to evaluate the inhibition activity of Tribulus terrestris L. (T. terrestris) fruits extracts with solvents of increasing polarity against…

Abstract

Purpose

The purpose of this paper is to evaluate the inhibition activity of Tribulus terrestris L. (T. terrestris) fruits extracts with solvents of increasing polarity against α-glucosidase and α-amylase, and to determine the inhibition mode of the most effective extract against both enzymes.

Design/methodology/approach

Hexane, acetone, ethanol and aqueous extracts of T. terrestris fruits were prepared using ultrasonic sequential extraction and analyzed for their α-amylase and α-glucosidase inhibitory activities by specific assay for each enzyme. The modes of inhibitions were detected using Lineweaver–Burk plots.

Findings

T. terrestris fruits extracts showed inhibition activity against α-glucosidase and α-amylase which was in the dose-dependent manner. Hexane extract had the highest α-glucosidase inhibition activity (IC50 = 27.28 μg/ml, p =0.003), followed by acetone and ethanol extracts (IC50 = 60.58 μg/ml and IC50 = 84.21 μg/ml, respectively). The inhibition mode of hexane extract was noncompetitive. While acetone extract showed the highest inhibition activity against α-amylase (IC50 = 6.18 mg/ml, p =0.002), hexane and ethanol extracts showed no significant difference (IC50 = 13.04 mg/ml and IC50 = 14.20 mg/ml, respectively, p =0.09). The inhibition mode of acetone extract was competitive.

Originality/value

T. terrestris fruits extracts had strong inhibition activity against α-glucosidase and α-amylase, and they can be used as a promising anti-diabetic agent.

Details

Nutrition & Food Science , vol. 50 no. 4
Type: Research Article
ISSN: 0034-6659

Keywords

Article
Publication date: 26 October 2012

Sukriti Das and Bratati De

The purpose of this paper is to determine the α‐amylase and α‐glucosidase inhibitory properties of grains of some red and white varieties of rice from India.

Abstract

Purpose

The purpose of this paper is to determine the α‐amylase and α‐glucosidase inhibitory properties of grains of some red and white varieties of rice from India.

Design/methodology/approach

Methanolic extracts of different rice varieties were analyzed for their α‐amylase and α‐glucosidase inhibitory properties. Total phenol and total anthocyanin contents were measured.

Findings

The white varieties of rice grains showed weak α‐glucosidase inhibitory activity and no α‐amylase inhibitory activity. The red varieties of rice grains showed good α‐glucosidase and α‐amylase inhibitory activities. The activities in these red varieties of rice, as determined by the IC50 values, were found to be correlated to total anthocyanin content. α‐glucosidase inhibitory activity of cyanidin‐3‐glucoside, the anthocyanin reported from rice grains was studied. But the IC50 values of different red varieties of rice were much less than that of cyanidin‐3‐glucoside indicating higher activity of red rice grain extracts than the purified pigment. This might be due to synergistic activity with other components present in rice extract.

Originality/value

The report shows hypoglycaemic potential of red varieties of rice grains.

Details

Nutrition & Food Science, vol. 42 no. 6
Type: Research Article
ISSN: 0034-6659

Keywords

Open Access
Article
Publication date: 3 June 2019

Lutendo Patricia Mathivha, Vuyisile Samuel Thibane and Fhatuwani Nixwell Mudau

The purpose of this paper is to investigate the health and medicinal importance of bush tea (Athrixia phylicoides DC) and special tea (Monsonia burkeana Planch. ex Harv), two of…

1983

Abstract

Purpose

The purpose of this paper is to investigate the health and medicinal importance of bush tea (Athrixia phylicoides DC) and special tea (Monsonia burkeana Planch. ex Harv), two of Southern African indigenous herbal teas.

Design/methodology/approach

The two herbal teas, A. phylicoides and M. burkeana were extracted individually and in combined ratios for analysis. The phenolic content was determined and the different phenolic compounds were identified using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The anti-diabetic activity of the teas was determined by evaluating the inhibition of both α-amylase and α-glucosidase in vitro. The anti-proliferative activity was measured on human cervical cancer (HeLa) cell line using the MTT (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium) assay.

Findings

Gallic acid, chlorogenic acid and quercetin were identified to be present in significant quantities by TLC. The HPLC quantified the presence of catechin (1.567 mg/g) and chlorogenic acid (1.862 mg/g) in special tea while chlorogenic acid (1.288 mg/g) was present in bush tea. Bush tea and special tea expressed significant levels of phenolic content and high antioxidant activities. Special tea (S100) expressed high inhibition of α-amylase, α-glucosidase and HeLa cell line proliferation when compared to bush tea (B100).

Originality/value

Both bush tea and special tea could provide an alternative for treatment and management of both diabetes and cervical cancer. However, future studies are needed to investigate their synergistic effect with a wide range of other commercial herbal teas.

Details

British Food Journal, vol. 121 no. 4
Type: Research Article
ISSN: 0007-070X

Keywords

Article
Publication date: 28 June 2013

Vellingiri Vadivel and Hans Konrad Biesalski

The purpose of this paper is to evaluate the antioxidant and type II diabetes related enzyme inhibition properties of phenolic extract from raw and traditionally processed Indian…

Abstract

Purpose

The purpose of this paper is to evaluate the antioxidant and type II diabetes related enzyme inhibition properties of phenolic extract from raw and traditionally processed Indian under‐utilized food legume grains, Bauhinia purpurea L. (purple camel's foot seeds).

Design/methodology/approach

The methanolic extract was prepared from the raw and traditionally processed seed samples and analyzed for total phenolic content. The antioxidant activity and type II diabetes related enzyme inhibition properties of methanolic extract and their relationship with phenolic content was demonstrated.

Findings

The methanolic extract of raw seed materials contained total free phenolic content of 14.45±1.62 g catechin equivalent/100 g extract DM. Encouraging levels of ferric reducing/antioxidant power (FRAP, 1203 mmol Fe[II]/mg extract), inhibition of ß‐carotene degradation (45.37 percent) and scavenging activity against DPPH (63.60 percent) and superoxide (42.14 percent) radicals were exhibited by the raw samples. Further, it also recorded 80.69 percent of α‐amylase and 63.74 percent of α‐glucosidase enzyme inhibition characteristics under in vitro starch digestion bioassay. Sprouting+oil‐frying caused an apparent increase on the total free phenolic content, antioxidant and free radical scavenging capacity, while soaking+cooking as well as open‐pan roasting treatments show diminishing effects.

Originality/value

Identification of suitable processing technique offered a good strategy to improve the phenolic content and health relevant functionality of B. purpurea seeds, which could be envisaged as a dietary ingredient in the formulation of supplementary foods with therapeutic value to manage type II diabetic patients.

Article
Publication date: 20 December 2023

Prapti Behera, Kannan N., Priyodip Paul, Sanjukta Aravind and Balaji S.

The textile sector struggles with cotton stickiness from honeydew contamination. It hurts agriculture and marketability. This study aims to examine how bacterial enzymes could…

Abstract

Purpose

The textile sector struggles with cotton stickiness from honeydew contamination. It hurts agriculture and marketability. This study aims to examine how bacterial enzymes could reduce honeydew-contaminated cotton adherence in textile businesses sustainably.

Design/methodology/approach

Enzyme was extracted from bacteria isolated from the fermented bamboo shoots “Lung siej”. The enzyme was tested for α-glucosidase using p-nitrophenyl-α-D-glucopyranoside as a substrate. Design of experiments determined enzyme activity temperature and reaction time. Laboratory-prepared artificial honeydew was added to ginning mill cotton to show honeydew contamination. After enzyme treatment, sticky cotton was tested for microscopic examination, ultraviolet (UV), Benedict’s, Elsner colorimetric, high volume instrument (HVI) and viscosity tests.

Findings

The bacterial isolate is characterized as Lysinibacillus sp. as confirmed by 16S rRNA gene sequencing. The enzyme extracted was identified as α-glucosidase. The ideal temperature and reaction time for enzymatic activity were 32 °C and 35 min, respectively, using central composite design. The microscopic examination, UV test, Benedict’s test, Elsner colorimetric test, HVI test and viscosity test showed that bacterial enzyme treatment reduced cotton fiber adherence.

Originality/value

Although few patents have examined the effect of yeast enzymes, to the best of the authors’ knowledge, a bacterial enzyme is investigated for the first time to reduce the adhesion of honeydew-contaminated cotton.

Details

Research Journal of Textile and Apparel, vol. ahead-of-print no. ahead-of-print
Type: Research Article
ISSN: 1560-6074

Keywords

Article
Publication date: 11 November 2019

Seok-Tyug Tan, Amin Ismail, Muhajir Hamid, Pei-Pei Chong, Jian Sun and Seok-Shin Tan

Literature has shown that phenolic acids and flavonoids are bearing with hypoglycemic and anti-adipogenic properties. Therefore, this study aims to evaluate the possibility of…

Abstract

Purpose

Literature has shown that phenolic acids and flavonoids are bearing with hypoglycemic and anti-adipogenic properties. Therefore, this study aims to evaluate the possibility of phenolic-rich soya bean husk powder extract (SHPE) in combating diabetes and obesity using in vitro models.

Design/methodology/approach

The hypoglycemic properties were evaluated by determining the ability of SHPE (25-100 µg/mL) in inhibiting a-amylase and a-glucosidase enzymes and in triggering insulin secretion in BRIN-BD11 cells. Murine 3T3-L1 adipocytes were used for evaluating the anti-adipogenic properties of SHPE through the determination of relative lipid accumulation, triglyceride content and glycerol-3-phosphate dehydrogenase (GPDH) activity.

Findings

The hypoglycemic properties of SHPE was in the dose-dependent manner, where 100 µg SHPE/mL exhibited a significant higher (p < 0.05) a-amylase inhibitory activity (56.8 ± 0.11 per cent) and insulin secretion activity (0.73 ± 0.02 µg/l) against other concentrations. In contrast to the aforementioned findings, a significant lower a-glucosidase inhibitory activity (52.0 ± 0.44 per cent) was also observed in 100 µg SHPE/mL. Nevertheless, findings revealed that all the SHPE were able to inhibit the activity of a-amylase and a-glucosidase and stimulated the insulin secretion in BRIN-BD11 cells. On the other hand, the anti-adipogenic properties of SHPE were in the reverse dose-dependent manner, where 100 µg SHPE/mL demonstrated a significant lower (p < 0.05) relative lipid accumulation (48.5 ± 0.03 per cent), intracellular triglyceride content (5.7 ± 0.07 mg/dL) and GPDH activity (1.0 ± 0.01 mU/mL). These findings reflected that 100 µg SHPE/mL was a potent anti-adipogenic agent when compared with other concentrations. In conclusion, soya husk could emerge as a potential hypoglycemic and anti-adipogenic agents in in vitro models.

Originality/value

This was the first study to explore the effectiveness of phytochemicals derived from soya bean husk in ameliorating hyperglycemia and adipogenesis. Promising findings that derived from the present study could enable the scientists to re-evaluate the potential use of agricultural wastes, especially in the formulation of nutraceuticals.

Details

Nutrition & Food Science , vol. 49 no. 6
Type: Research Article
ISSN: 0034-6659

Keywords

Article
Publication date: 9 July 2019

Mehdi Fasihi, Mohammad Yousefi, Abdolrasoul Safaiyan, Mahdi Mousavi Mele, Mohammadreza Rostami and Ali Barzegar

The purpose of this paper is to examine the effect of green coffee extract on anthropometric index and lipid profile, fasting blood sugar, chemerin and malondialdehyde on subjects…

Abstract

Purpose

The purpose of this paper is to examine the effect of green coffee extract on anthropometric index and lipid profile, fasting blood sugar, chemerin and malondialdehyde on subjects with metabolic syndrome.

Design/methodology/approach

A randomized, double-blind, placebo-controlled clinical trial was conducted in Sheikh Al Raise Clinic from September 2016 to March 2017. The participants were randomly divided into green coffee group and placebo group. Green coffee group (n = 24) received green coffee extract (GCE), while placebo group (n = 24) took cellulose as a placebo, two capsules (400 mg) two times each day for eight weeks. The anthropometric index and lipid profile, fasting blood sugar, chemerin and malondialdehyde were measured at the beginning of the study and after eight weeks of treatment with GCE. Blood samples were collected before and after eight weeks of supplementation.

Findings

Significant weight loss, from 84.80 ± 2.12 kg to 80.94 ± 2.10 kg (ptime = 0.030, pGC = 0.007), as well as decreases in body mass index (ptime = 0.034, pGC = 0.006) were detected in the green coffee group after eight weeks. Also, the green coffee group has significant lower (pgroup = 0.029, ptime = 0.013) malondialdehyde (MDA) compared to the placebo group, and there was a significant difference between two groups at the insulin level and homeostatic model assessment of insulin resistance (HOMA-IR) (ptime = 0.001, pgroup = 0.048), (ptime = 0.012, pgroup = 0.007). However, there was no significant difference in lipid profile, fasting blood sugar and serum chemerin between two groups after eight weeks of supplementation.

Originality/value

This paper showed the statistical difference in body weight, malondialdehyde, insulin and insulin resistance after eight weeks of treatment. GCE might be associated to reduction in the carbohydrate absorption and the enhancement of lipid metabolism.

Details

Nutrition & Food Science , vol. 50 no. 1
Type: Research Article
ISSN: 0034-6659

Keywords

Article
Publication date: 14 July 2020

Shonima Venugopal, Uma Iyer and Richa Sanghvi

Glycemic index (GI) is a physiological basis for ranking carbohydrate foods based on the blood glucose responses they produce after ingestion. Emblica officinalis (E. officinalis

Abstract

Purpose

Glycemic index (GI) is a physiological basis for ranking carbohydrate foods based on the blood glucose responses they produce after ingestion. Emblica officinalis (E. officinalis) is a medicinal plant that purportedly has hypoglycaemic and hypolipidemic properties. This study aims to determine the glycemic and lipemic responses of freeze-dried E. officinalis powder-incorporated recipes.

Design/methodology/approach

Two sets of four equicarbohydrate (50 g) recipes (vegetable cutlet, handvo, muthiya and methi thepla) were developed, one without E. officinalis powder incorporation (standard) and one with E. officinalis powder incorporation at the 2 g level (test). After overnight fasting, 50 g glucose, standard and test recipes were administered to healthy adult volunteers at different instances (each 3–4 days apart) and blood glucose levels were measured using capillary sampling every 15 min for 2 h. The glycemic response and GI values were then calculated.

Findings

Among the standard recipes, lowest glycemic response was obtained by methi thepla (60.90 ± 15.54) and highest glycemic response by handvo (90.57 ± 33.88). Incorporation of E. officinalis powder brought about a non-significant reduction in the GI of methi thepla (p = 0.94), vegetable cutlet (p = 0.54), muthiya (p = 0.69) and handvo (p = 0.09). Maximum per cent reduction was for handvo, which shifted from the high to medium GI category. The lipemic response was lowest with muthiya, showing a fall in triacylglycerol (TG) levels (3.9%). E. officinalis powder incorporation in muthiya led to a further fall (7.8%) in TG levels.

Originality/value

Incorporation of freeze-dried E. officinalis powder in Indian recipes can bring about a reduction in the postprandial glycemic and lipemic responses.

Details

Nutrition & Food Science , vol. 51 no. 3
Type: Research Article
ISSN: 0034-6659

Keywords

Article
Publication date: 7 September 2018

S. Sarkar

Yoghurt is most popular and more acceptable throughout the world because of its general positive image among consumers because of its diverse nutritional and therapeutic…

3389

Abstract

Purpose

Yoghurt is most popular and more acceptable throughout the world because of its general positive image among consumers because of its diverse nutritional and therapeutic properties and can be the most suitable probiotic carrier. Key factors for consumer’s inclination towards functional foods are increased awareness for healthy foods because of health deterioration resulting from busy lifestyles, growing healthcare cost and the aspiration for an improved quality life in later years. Yoghurt is still not consumed in certain parts of the world because of a lack of a cultural tradition of consuming yogurt and further people are not aware of the health benefits associated with yogurt consumption. In this study an attempt has been to project probiotic yoghurt as a functional food in the current era of self-care and complementary medicine.

Design/methodology/approach

Attempt has been made to review the literature on the biochemical activities of yoghurt cultures and their behavior in association with diverse probiotic cultures. Both review and research papers related to biochemical activities and functional properties of yoghurt cultures in association with probiotics and their health benefits published in diverse journals under Pub Med and Science Direct have been considered. Keywords used for data search included functional foods, yoghurt, probiotic, health benefits, etc.

Findings

Functional properties of yoghurt can be further enhanced with fortification of minerals and vitamins or inclusion of probiotic cultures. Diversity in biochemical behavior yoghurt cultures in association with different probiotic cultures has been reported. Conjugated application of probiotics with yoghurt cultures would result in a product with enhanced functional properties to extend health benefits.

Originality/value

Inclusion of probiotic cultures in yoghurt is suggested to extend the functional properties of normal yoghurt, thus providing necessary nutrients, improving health and preventing or reducing nutrition-related diseases. Regular intake of probiotic yoghurt is suggested for healthy lifestyles, as it will help in retaining their health and reduce the potentially long-term risk of disease. Food industries can have profit-driven business by projecting the probiotic yoghurt as a functional food.

Details

Nutrition & Food Science, vol. 49 no. 2
Type: Research Article
ISSN: 0034-6659

Keywords

Article
Publication date: 28 December 2021

Modupeola Oguntoye, Olufunke Ezekiel and Olayinka Oridupa

This study aims to evaluate the effect of probiotic provitamin A cassava hydrolysate with Lactobacillus rhamnosus GG (hLGG) on weight and lipid profile of Wistar rats and its…

Abstract

Purpose

This study aims to evaluate the effect of probiotic provitamin A cassava hydrolysate with Lactobacillus rhamnosus GG (hLGG) on weight and lipid profile of Wistar rats and its glycemic index using Wistar rats and human subjects.

Design/methodology/approach

Adult male Wistar rats (n = 40, 120–150 g) were orally administered provitamin A cassava hydrolysate with 1 × 1010, 2 × 1010 and 4 × 1010 CFU/g encapsulated or CFU/mL free Lactobacillus rhamnosus GG for 30 days, during which weight and lipid profile of rats were monitored. Blood glucose levels of rats and human subjects were also measured in Oral Glucose Tolerance Test to determine the Glycemic indices of hLGG.

Findings

Rats administered the highest doses of free or encapsulated hLGG [(4 × 1010 CFU) (PHE4 and PHF4, respectively)] had the lowest (18.2 ± 0.7 and 8.0 ± 0.6%, respectively, p < 0.001) percentage body weight gain compared to control (40 ± 0.6%). Lowest cholesterol and triglyceride (42.4 ± 0.5 and 44.4 ± 0.7 g/dL, p < 0.001, respectively) were observed in rats administered PHE4, with the lowest plasma glucose concentrations in PHE4 and PHF4 groups (43 ± 1 and 49 ± 0.7 g/dL, p < 0.001, respectively). Oral Glucose Tolerance Test for rats and human subjects showed lower peak blood glucose levels and glycemic indices in hLGG groups compared to controls in a dose-dependent manner.

Originality/value

Consumption of soft drinks, which supply non-nutritive energy, may lead to degenerative metabolic disorders such as obesity and diabetes. Beverages with probiotics such as Lactobacillus rhamnosus GG, on the other hand, offer a positive weight management approach. Development of non-dairy beverages such as provitamin A cassava hLGG is ongoing. Provitamin A cassava hLGG showed its ability to control weight gain, blood glucose levels and serum lipids. Thus, the beverage can be consumed as a healthy alternative to soft drinks and for weight management.

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