TY - JOUR AB - The production of biohydrogen from rice mill wastes, including rice bran and rice mill effluent by Clostridium acetobutylicum NCIM 2877 was investigated in a batch culture system and optimized the temperature and pH conditions. At 35 °C with initial pH of 5.2 a yield of 55.7±0.8 ml with 88.6 ± 0.3% substrate utilization and at pH 6 the production was 68.7 ± 0.9 ml with 85 ± 1.0 % substrate utilization. Addition of metal ions resulted in better yield and substrate utilization efficiency of the bacteria. FeSO4.7H2O at a concentration of 50 mg/l gave 79.7 ± 1.5 ml with 96% of substrate utilization. Cobalt effected the production greatly by giving 96.3 ± 0.9 ml with 95.3 ± 0.7% of substrate utilization whereas Nickel didn’t showed much effective results by giving only a maximum of 74.7 ± 1.5 ml production at 25 mg/l concentration. Conclusively, it can be stated that rice mill wastes can be used as a substrate and use of metal ions will play a key role in the enhancement of biohydrogen production. VL - 12 IS - 4 SN - 1708-5284 DO - 10.1260/1708-5284.12.4.383 UR - https://doi.org/10.1260/1708-5284.12.4.383 AU - Shukla Garima AU - Thakur Veena AU - S.K. Jadhav PY - 2015 Y1 - 2015/01/01 TI - Biohydrogen production from rice mill wastes by Clostridium acetobutylicum NCIM 2877 T2 - World Journal of Engineering PB - Emerald Group Publishing Limited SP - 383 EP - 390 Y2 - 2024/04/25 ER -