Identification of ß -carotene producing bacteria from terrestrial habitats of Jaipur, Rajasthan
ISSN: 0369-9420
Article publication date: 25 June 2019
Issue publication date: 5 July 2019
Abstract
Purpose
Carotenoids are pigments that have significant value as colorants and antioxidants in pharmaceutical, food, textile and cosmetic industries. Owing to its high demand, this study aims to identify ß-carotene producing bacteria from different terrestrial habitats of Jaipur region.
Design/methodology/approach
In this paper, standard isolation and purification process was used, followed by colony morphology and biochemical characterization of ß-carotene producing bacteria. ß-carotene concentration was determined quantitatively using spectrophotometric method.
Findings
Out of 43 isolates, 21 isolates showed peak range between 400 and 500 nm confirming the presence of carotenoids. Only one bacteria SAN-A has capacity to produce ß-carotene confirmed by the thin layer chromatography and high performance liquid chromatography (HPLC) with a yield of 1.68 mg/l. The 2, 2-Diphenyl-2-picrylhydrazil (DPPH) assay showed an IC50 value of 4.0 mg/ml.
Originality/value
The present study revealed the presence of ß-carotene producing bacteria in the soil of different terrestrial habitat of Jaipur region which can be exploited as an economical source for ß-carotene production.
Keywords
Acknowledgements
Funding of this research program was provided by ICMR File no. 3/1/2/53/2014-2015 (Nut.) New Delhi and the facility for this work was supported by the Deparment of Biosciences, Manipal University Jaipur.
Conflict of interest: The authors report no conflicts of interest.
Erratum: It has been brought to the attention of the publisher that in the article Ankit Chokriwal, Bjorn John Stephen and Abhijeet Singh “Identification of ß-Carotene producing bacteria from terrestrial habitats of Jaipur, Rajasthan”, published in Pigment & Resin Technology, incorrectly listed the authors affiliation as “Department of Biosciences, Manipal University, Jaipur, India”. This has now been corrected, the publisher apologises for any inconvenience caused.
Citation
Chokriwal, A., Stephen, B.J. and Singh, A. (2019), "Identification of
Publisher
:Emerald Publishing Limited
Copyright © 2019, Emerald Publishing Limited