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Identification of crocin, crocetin and zeaxanthin in Crocus sativus grown under controlled environment in Malaysia

Nordiyanah Anuar (Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia)
Rosna Mat Taha (Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia)
Noraini Mahmad (Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia)
Rashidi Othman (Department of Landscape Architecture, Kulliyyah of Architecture and Environment Design, International Islamic University, Kuala Lumpur, Malaysia)

Pigment & Resin Technology

ISSN: 0369-9420

Article publication date: 21 August 2018

Issue publication date: 7 November 2018

94

Abstract

Purpose

The purpose of the study is to identify the high valuable compounds which are crocin, crocetin and zeaxanthin in the stigmas and stamens of Crocus sativus grown under controlled environment in Malaysia.

Design/methodology/approach

Spectrophotometry and high-performance liquid chromatography (HPLC) analysis were used to identify and measure crocin, crocetin and zeaxanthin content qualitatively and quantitatively in the stigmas and stamens of C. sativus grown under controlled environment in Malaysia.

Findings

The results of this study showed that crocin, crocetin and zeaxanthin were detected in the stigmas. However, among those three compounds, only crocetin was detected in the stamens. In the stigmas, the detectable level of crocin was high compared to crocetin and zeaxanthin. It was also found that crocetin was higher in the stamens compared to in the stigmas.

Originality/value

This is the first attempt in Malaysia that the stigmas and stamens were directly purified from the natural sources by means of no addition of preservatives as C. sativus has never been grown here before. Furthermore, limited reports are available regarding the identification of compounds in saffron stamens.

Keywords

Acknowledgements

The authors would like to thank the University of Malaya for the facilities and financial supported by the Institute of Research Management and Monitoring, IPPP (Postgraduate Grant – PG175-2016A) and University of Malaya Research Grant, UMRG (RP024A-14AFR).

Citation

Anuar, N., Taha, R.M., Mahmad, N. and Othman, R. (2018), "Identification of crocin, crocetin and zeaxanthin in Crocus sativus grown under controlled environment in Malaysia", Pigment & Resin Technology, Vol. 47 No. 6, pp. 502-506. https://doi.org/10.1108/PRT-11-2016-0107

Publisher

:

Emerald Publishing Limited

Copyright © 2018, Emerald Publishing Limited

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