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Detection and quantification of natural pigments extracted from callus of Echinocereus cinerascens

Hashimah Elias (Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia and Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia)
Rosna Mat Taha (Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia)
Nor Azlina Hasbullah (Department of Agricultural Sciences, Faculty of Technical and Vocational, Tanjong Malim, Perak, Malaysia)
Rashidi Othman (Department of Landscape Architecture, International Islamic University Malaysia, Kuala Lumpur, Malaysia)
Noraini Mahmad (Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia)
Azani Saleh (Faculty of Applied Sciences, MARA University of Technology, Shah Alam, Selangor, Malaysia)
Sakinah Abdullah (Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia)

Pigment & Resin Technology

ISSN: 0369-9420

Article publication date: 18 October 2018

Issue publication date: 7 November 2018

201

Abstract

Purpose

This paper aims to study the effect of different organic solvents on the extraction of pigments present in callus cultures of E. cinerascens.

Design/methodology/approach

Attempts have been made to extract pigments from callus cultures through tissue culture system as an alternative replacement for conventional plant cultivation as tissue culture provides unlimited supplies of plant samples. Callus of E. cinerascens was induced from stem explant cultured in Murashige and Skoog medium supplemented with combination of 0.5 mg/L 6-benzylaminopurine and 0.5 mg/L α-naphthaleneacetic acid maintained under photoperiod of 16 h light and 8 h dark. Fresh samples of the callus were harvested and dissolved in various types and concentrations of solvents such as 100 per cent acetone, 80 per cent acetone, 95 per cent ethanol, 100 per cent methanol and 90 per cent methanol. Each of the mixtures was directly centrifuged to get clear supernatant containing pigments of interest. The pigments were detected and subsequently quantified via two simple techniques, ultraviolet-visible (UV-Vis) spectrophotometer and thin layer chromatography (TLC).

Findings

UV-Vis spectrophotometer detected two families of pigments present in the callus cultures, namely, carotenoids (carotene and xanthophyll) and tetrapyrroles (chlorophyll a and b). Pigment contents in various solvent extractions were estimated using spectroscopic quantification equations established. Through TLC, spots were seen on the plates, and Rf values of each spots were assessed to indicate the possible existence of carotenoids and tetrapyrroles.

Originality/value

This preliminary study offers significant finding for further advance research related on natural pigments extracted from E. cinerascens that would provide profits in the future applications, especially in food industry, medicine, agriculture, etc.

Keywords

Acknowledgements

The authors would like to thank University of Malaya for the financial support (PV25/2011B) and the facilities provided.

Citation

Elias, H., Mat Taha, R., Hasbullah, N.A., Othman, R., Mahmad, N., Saleh, A. and Abdullah, S. (2018), "Detection and quantification of natural pigments extracted from callus of Echinocereus cinerascens", Pigment & Resin Technology, Vol. 47 No. 6, pp. 464-469. https://doi.org/10.1108/PRT-11-2016-0103

Publisher

:

Emerald Publishing Limited

Copyright © 2018, Emerald Publishing Limited

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