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A highly sensitive and quantitative detection method for bisphenol A (BPA) by competitive immunoassay based on surface-enhanced Raman spectroscopy

Zhang Lei (Guangdong University of Technology, Guangzhou, China)
Yingshan Chen (Guangdong University of Technology, Guangzhou, China)
Zhiwen Liu (Guangdong University of Technology, Guangzhou, China)
Wenjin Ji (School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, China)
Suqing Zhao (Guangdong University of Technology, Guangzhou, China)

Pigment & Resin Technology

ISSN: 0369-9420

Article publication date: 2 January 2018

Abstract

Purpose

In this study, a highly sensitive and quantitative analysis method using surface-enhanced Raman scattering (SERS)-labeled immunoassay is adopted for bisphenol A bisphenol A (BPA) detection in water samples.

Design/methodology/approach

Primarily, an excellent SERS immuno-nanoprobe is prepared, which relays on Au/Ag core-shell nanoparticles tagged 4-mercaptobenzoic acid (4MBA) and labeled with specific antibody against BPA. Second, the coating antigen of 4,4-Bis(4-hydroxyphenol) valeric acid (BVA) coupling poly-L-lysine (PLL) conjugate (BVA-PLL) is fastened on the substrate. Based on competitive immunoassay, the antibody labeled on SERS immuno-nanoprobe will bind with the free BPA and BVA-PLL competitively.

Findings

A calibration curve was obtained by plotting the intensity of SERS signal of 4MBA at 1007 cm−1 versus the concentration of BPA. The results indicated that the limit of detection (LOD) for BPA is 1 ng/mL and present a great capacity for higher sensitivity. Furthermore, the method was able to quantitatively detect BPA in water samples, which was validated by high performance liquid chromatography (HPLC).

Originality/value

The method was developed based on competitive immunoassay, and the conjugate (BVA-PLL) was chosen as the coating antigen. Au/Ag core-shell nanoparticles played as the SERS active substrate and were labeled with Raman reporter. The value of this paper is supplying a wide potential for analysis of target analytes in the environmental monitoring and food safety.

Keywords

Acknowledgements

Conflict of interest: The authors declare no conflict of interest.

This research was supported by the National Natural Science Foundation of China (41271340) and Guangdong province Natural Science Fund (S2013030013338).

Citation

Lei, Z., Chen, Y., Liu, Z., Ji, W. and Zhao, S. (2018), "A highly sensitive and quantitative detection method for bisphenol A (BPA) by competitive immunoassay based on surface-enhanced Raman spectroscopy", Pigment & Resin Technology, Vol. 47 No. 1, pp. 38-46. https://doi.org/10.1108/PRT-03-2017-0022

Publisher

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Emerald Publishing Limited

Copyright © 2018, Emerald Publishing Limited