The purpose of this paper is to improve the generation efficiency of singlet oxygen of methylene blue molecules through finely controlling their aggregation states in drug carriers.
As a photosensitiser in photodynamic therapy, methylene blue (MB) was loaded on citrate-modified hydroxyapatite (HAp) through an electrostatic interaction and followed by encapsulation of coordination complexes of tannic acid (TA) and Fe(III) ions. Ultraviolet-visible absorption spectrum of the supernatant after incubation of samples was recorded at certain time interval to investigate the release behaviour of MB. Photodynamic activity of MB was determined by the oxidation reaction of uric acid by singlet oxygen generated by MB under illumination.
Almost all MB molecules were immediately released from HAp-MB, whilst an initial burst release of MB from HAp-MB@TA was followed by a sustainable and pH-sensitised release. In comparison with HAp-MB, photocatalystic reduction of HAp-MB@TA by titanium dioxide hardly occurred under illumination, indicating the stability against reduction to leukomethylene blue in vitro. Generation efficiency of singlet oxygen by MB released from HAp-MB@TA was significantly higher than that from HAp-MB because of the control of TA and Fe(III) ions complexes on molecular structures of released MB.
A facile method was herein demonstrated to optimise the generation efficiency of singlet oxygen by controlling aggregation states of PS molecules and improve PDT efficiency to damage tumour tissues.
The study was supported by the Natural Science Foundation, China (Grant No. 51702189 and 11704226), and the Shandong Provincial Natural Science Foundation, China (Grant No. ZR2017BEM033 and ZR2017MA051).
Wang, W., Shi, R., Zhang, W., Sun, H., Ge, X. and Li, C. (2019), "Enhanced generation efficiency of singlet oxygen for methylene blue released from hydroxyapatite-MB@tannic acid-Fe(III) ions", Pigment & Resin Technology, Vol. 48 No. 2, pp. 185-196. https://doi.org/10.1108/PRT-02-2018-0011Download as .RIS
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