Design and synthesis of thermosensitive block resin as carrier for immobilization of penicillin G acylase
ISSN: 0369-9420
Article publication date: 1 December 2021
Issue publication date: 22 July 2022
Abstract
Purpose
Using a reversible addition fragmentation chain transfer reaction, a series of resins were prepared by using N, N-diethyl acrylamide (DEA), poly (ß-hydroxyethyl methacrylate) (PHEMA) as hydrophilic blocks and poly (glycidyl methacrylate) (PGMA) as hydrophobic blocks (and as a target for immobilizing penicillin G acylase [PGA]) and the low critical solution temperature (LCST) of which could be adjusted by changing the segment length of blocks.
Design/methodology/approach
To make the catalytic conversion temperature of immobilized PGA fallen into the temperature range of the sol state of thermosensitive block resin, a type of thermosensitive block resin, i.e. PDEA-b-PHEMA-b-PGMA (DHGs) was synthesized to immobilize PGA, and the effect of segment order of block resin was investigated on the performance of PGA.
Findings
Carrier prepared with monomers molar ratio of n(DEA) : n(HEMA): n(GMA) = 100: 49: 36 presented loading capacity (L) and enzyme activity recovery ratio (Ar) of 110 mg/g and 90%, respectively, and a block resin with LCST value of 33 °C was essential for keeping higher Ar of PGA.
Originality/value
PGA has become an important biocatalyst in modern chemistry industry. However, disadvantages include difficulty in separation, poor repeatability and high cost, which limits the scope of PGA applications. The effective method is to immobilize the enzyme to the carrier, which could overcome the disadvantage of free enzyme.
Keywords
Acknowledgements
Author contributions: #These authors contributed equally to this work.
This work was supported by the National Natural Science Foundation of China (Grant No. 51563015).
Citation
Tu, H., Liu, D., Chen, Z. and Liu, C. (2022), "Design and synthesis of thermosensitive block resin as carrier for immobilization of penicillin G acylase", Pigment & Resin Technology, Vol. 51 No. 5, pp. 499-507. https://doi.org/10.1108/PRT-01-2021-0011
Publisher
:Emerald Publishing Limited
Copyright © 2021, Emerald Publishing Limited