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Antioxidant activity of polyphenols from larch wood: an amperometric study

Alexander Y. Yashin (International Analytical Center, N.D. Zelinsky Institute of Organic Chemistry, Moscow, Russia)
Dmitry V. Yashunsky (Laboratory of Glycoconjugate Chemistry, N.D. Zelensky Institute of Organic Chemistry, Moscow, Russia)
Aleksander N. Vedenin (International Analytical Center, N.D. Zelensky Institute of Organic Chemistry, Moscow, Russia)
Nikolay E. Nifantiev (Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Moscow, Russia)
Yakov I. Yashin (International Analytical Center, N.D. Zelinsky Institute of Organic Chemistry, Moscow, Russia)
Boris V. Nemzer (Research and Development, FutureCeuticals, Inc., Momence, Illinois, USA, and Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA)

Nutrition & Food Science

ISSN: 0034-6659

Article publication date: 13 March 2017

127

Abstract

Purpose

The purpose of this paper is to determine the antioxidant activity of natural polyphenols from larch wood by the amperometric method.

Design/methodology/approach

Direct measurements of antioxidant activity were carried out by the amperometric method in an oxidizing mode with glassy carbon as a working electrode, set potential +1.3V and using a flow-injection system with 2.2 mM phosphoric acid as the mobile phase with a flow rate of 1.2 ml/min.

Findings

The reported results show the following values of antioxidant activity for the tested compounds: (−)-secoisolariciresinol – 0.199 ± 0.002 mg/L (p < 0.05); isolariciresinol – 0.196 ± 0.002 mg/L (p < 0.05); lariciresinol - 0.222 ± 0.001 mg/L (p < 0.05); O-isopropylidene derivative of (−)-secoisolariciresinol - 0.143 ± 0.002 mg/L (p < 0.05); (+)-dihydroquercetin – 0.153 ± 0.002 mg/L (p < 0.05); and quercetin – 0.521 ± 0.001 mg/L (p < 0.05). The last product was tested as the reference of a widely used current antioxidant. General tendencies of determined values of antioxidant activity for studied compounds are in good correlation with published data as determined by the t-BuOOH-initiated lipid peroxidation method.

Practical implications

Described results show practical applicability of the amperometric method as being faster and cheaper in comparison to other methods, including oxygen radical absorbance capacity (ORAC) assay or 2,2′diphenyl-1-picrylhydrazyl (DPPH) reagent based assay.

Originality/value

The described results show the first-time application of the amperometric method for the evaluation of the antioxidant activity of phenolic compounds from larch wood.

Keywords

Acknowledgements

This work was supported by the Russian Science Foundation (grant no. 14-50-00126).

Citation

Yashin, A.Y., Yashunsky, D.V., Vedenin, A.N., Nifantiev, N.E., Yashin, Y.I. and Nemzer, B.V. (2017), "Antioxidant activity of polyphenols from larch wood: an amperometric study", Nutrition & Food Science, Vol. 47 No. 2, pp. 297-303. https://doi.org/10.1108/NFS-05-2016-0064

Publisher

:

Emerald Publishing Limited

Copyright © 2017, Emerald Publishing Limited

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